| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 405 | NBP2-70135AFP405 | |||
| Alexa Fluor Plus 488 | NBP2-70135AFP488 | |||
| Alexa Fluor Plus 555 | NBP2-70135AFP555 | |||
| Alexa Fluor Plus 594 | NBP2-70135AFP594 | |||
| Alexa Fluor Plus 647 | NBP2-70135AFP647 | |||
| Alexa Fluor Plus 680 | NBP2-70135AFP680 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70135 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7419-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP2-21678 | |||
| DyLight 488 | NBP1-77701 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89159-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB17751-SP | |||
| Carrier Free | IC17751V-100UG | |||
| Carrier Free | IC17751U-100UG | |||
| Carrier Free | IC17751T-100UG | |||
| Carrier Free | IC17751S-100UG | |||
| Carrier Free | IC17751R-100UG | |||
| Carrier Free | IC17751N-100UG | |||
| Carrier Free | IC17751G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6815-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44500 | |||
| BSA Free | NBP2-52956-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21562-50ul | |||
![Western Blot: AKT1 Antibody (OTI4D6) [NBP2-01724] - WB analysis of extracts (35 ug) from 9 different cell lines using AKT1 antibody.](http://images.novusbio.com/fullsize/AKT1-Antibody-4D6-Western-Blot-NBP2-01724-img0009.jpg "Western Blot: AKT1 Antibody (OTI4D6) [NBP2-01724] - WB analysis of extracts (35 ug) from 9 different cell lines using AKT1 antibody.")
![Western Blot: AKT1 Antibody (OTI4D6) [NBP2-01724] - WB detection of AKT1 in human MD-MB-231 cells using AKT1 antibody NBP2-01724 at a dilution of 1:2000. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/AKT1-Antibody-4D6-Western-Blot-NBP2-01724-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 Antibody (OTI4D6) [NBP2-01724] - ICC/IF of COS7 cells transiently transfected by pCMV6-ENTRY AKT1.](http://images.novusbio.com/fullsize/AKT1-Antibody-4D6-Immunocytochemistry-Immunofluorescence-NBP2-01724-img0008.jpg)
![Western Blot: AKT1 Antibody (OTI4D6) [NBP2-01724] - WB analysis of extracts (10 ug) from a mouse cell line and 3 different mouse tissues using AKT1 antibody at a 1:200 dilution.](http://images.novusbio.com/fullsize/Akt1-Antibody-4D6-Western-Blot-NBP2-01724-img0012.jpg)
![Immunohistochemistry-Paraffin: AKT1 Antibody (OTI4D6) [NBP2-01724] - IHC-P staining of paraffin-embedded human prostate tissue using anti-AKT1 antibody. Heat-induced epitope retrieval in 10 mM citric buffer, pH 6.0, 100C for 10 min.](http://images.novusbio.com/fullsize/AKT1-Antibody-4D6-Immunohistochemistry-Paraffin-NBP2-01724-img0007.jpg)
![Flow Cytometry: AKT1 Antibody (OTI4D6) [NBP2-01724] - Flow cytometric analysis of Jurkat cells using anti-AKT1 antibody (red), compared to a nonspecific negative control antibody (blue).](http://images.novusbio.com/fullsize/AKT1-Antibody-4D6-Flow-Cytometry-NBP2-01724-img0001.jpg)
![Western Blot: AKT1 Antibody (OTI4D6) [NBP2-01724] - HEK293T cells were transfected with the pCMV6-ENTRY control (left lane) or pCMV6-ENTRY AKT1 (right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT1.](http://images.novusbio.com/fullsize/AKT1-Antibody-4D6-Western-Blot-NBP2-01724-img0002.jpg)
![Immunohistochemistry-Paraffin: AKT1 Antibody (OTI4D6) [NBP2-01724] - IHC-P staining of paraffin-embedded adenocarcinoma of human endometrium tissue using anti-AKT1 antibody. Heat-induced epitope retrieval in10 mM citric buffer, pH 6.0, 100C for 10 min.](http://images.novusbio.com/fullsize/AKT1-Antibody-4D6-Immunohistochemistry-Paraffin-NBP2-01724-img0003.jpg)
![Immunohistochemistry-Paraffin: AKT1 Antibody (OTI4D6) [NBP2-01724] - IHC-P staining of paraffin-embedded carcinoma of human bladder tissue using anti-AKT1 antibody. Heat-induced epitope retrieval in 10 mM citric buffer, pH 6.0, 100C for 10 min.](http://images.novusbio.com/fullsize/AKT1-Antibody-4D6-Immunohistochemistry-Paraffin-NBP2-01724-img0004.jpg)
![Immunohistochemistry-Paraffin: AKT1 Antibody (OTI4D6) [NBP2-01724] - IHC-P staining of paraffin-embedded carcinoma of human kidney tissue using AKT1 antibody. Heat-induced epitope retrieval in 10 mM citric buffer, pH 6.0, 100C for 10 min.](http://images.novusbio.com/fullsize/AKT1-Antibody-4D6-Immunohistochemistry-Paraffin-NBP2-01724-img0005.jpg)
![Immunohistochemistry-Paraffin: AKT1 Antibody (OTI4D6) [NBP2-01724] - IHC-P staining of paraffin-embedded human kidney tissue using anti-AKT1 antibody. Heat-induced epitope retrieval in 10 mM citric buffer, pH 6.0, 100C for 10 min.](http://images.novusbio.com/fullsize/AKT1-Antibody-4D6-Immunohistochemistry-Paraffin-NBP2-01724-img0006.jpg)
![AKT1 Antibody (OTI4D6)[NBP2-01724]Simple Western� analysis of endogenous protein BCAT1 from HepG2 lysates (0.5 mg/mL) using BCAT1 Mouse Monoclonal Antibody . The virtual lane view (left) shows the target (as indicated) at 1:20 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:20 dilutios of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.](http://images.novusbio.com/fullsize/nbp2-01724_mouse-akt1-mab-oti4d6-simple-western-131120251236921.jpg%253fstore=america&image-type=image)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - A431 cells. Panel A: serum starved, unstimulated cells. Panel B: serum starved, EGF stimulated for 15 mins. A massive increase in AKT-pS473 activation, as measured by intensity signal, peaked at 15 minutes and was associated with depolymerized tubulin. Panel A shows STED data (AKT-pS473, red channel) collected simultaneously with confocal signal (alpha-tubulin, green channel). Upon stimulation of cells with EGF, a rapid activation of AKT is observed (Panel B) along with a coincident change in the tubulin organization (yellow signal), as well as an extensive cell shape-change (cell membrane folding) and accumulation of AKT pS473 at the cell periphery.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0011.jpg "Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - A431 cells. Panel A: serum starved, unstimulated cells. Panel B: serum starved, EGF stimulated for 15 mins. A massive increase in AKT-pS473 activation, as measured by intensity signal, peaked at 15 minutes and was associated with depolymerized tubulin. Panel A shows STED data (AKT-pS473, red channel) collected simultaneously with confocal signal (alpha-tubulin, green channel). Upon stimulation of cells with EGF, a rapid activation of AKT is observed (Panel B) along with a coincident change in the tubulin organization (yellow signal), as well as an extensive cell shape-change (cell membrane folding) and accumulation of AKT pS473 at the cell periphery.")
![Flow Cytometry: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis using the DyLight 488 conjugate of AKT1 phospho Ser473 antibody. Image shows anti-histone detection using a DyLight 488 conjugate (green). Anti-Tubulin was detected using a DyLight 549 conjugate (red). Nuclei were counter-stained using DAPI (blue).](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Flow-Cytometry-NBP1-69923-img0021.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT1 [p Ser473] antibody (17F6.B11). A: Lane 1) PDGF stimulated NIH 3T3 cells [10ul]. Lane 2) NIH 3T3 cells [10ul]. Lane 3) Hela whole cell lysate [10ul] (weak signal). B: Lane 4) GST negative control protein [100ng]. Lane 5) GST negative control protein [25ng]. Lane 6) AKT 1 recombinant protein [100ng]. Lane 7) AKT 1 recombinant protein [25ng].Block: 5% BSA overnight at 4C.Primary antibody: Monoclonal anti-AKT antibody used at 1:1000 for overnight at 4C.Secondary antibody: HRP Conjugated goat anti-mouse 1:25K for 45 min at RT.Detection: TMB for 20 minutes, rinsed with deionized water, dried and scanned on conventional flatbed scanner.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0045.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - High resolution STED immunofluorescence nanoscopy of AKT11 [p Ser473] antibody (17F6.B11). Tissue: A431 cells. The merge images (A) and at high magnification (B) show phosphorylated AKT1 colocalized with the distal microtubules. Fixation: 4% paraformaldehyde for 5 min and after washes blocked with 10% NGS/0.2% Triton X-100 for 30 min.Antigen retrieval: serum deprivation for 12 h.Primary antibody: AKT1 pS473 antibody at 10 ug/mL and alpha-tubulin (cyan) at 1.4 ug/mL for 1 h at RT.Secondary antibody: Atto 647N anti-Mouse IgG (ATTO TEC GmbH), and DyLight(TM)488 anti-Rabbit IgG were used at 1.0 ug/mL for 1h at RT for indirect detection.Localization: AKT1 pS473 is in the cytoplasm and also organized at the periphery of the cell.Staining: AKT1 pS473 as red signal with bis-benzimide (blue) nuclear counterstain.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0040.jpg)
![Immunohistochemistry: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Immunohistochemistry of AKT11 [p Ser473] antibody (17F6.B11). Tissue: human prostate tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: AKT1 pS473 antibody at 20 ug/mL for 1 h at RT.Secondary antibody: Dako's Techmate streptavidin-biotin reagents at 1:10,000 for 45 min at RT.Localization: AKT1 pS473 is nuclear and occasionally cytoplasmic.Staining: AKT1 pS473 as precipitated red signal with hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunohistochemistry-NBP1-69923-img0046.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Immunoblotting analysis. Representative images of the immunoblot analysis for (A) Akt, (B) p53 (C) AMPK, and (D) LC3 at 24 h of incubation with or without 20 mM dG are shown. beta-actin was used as loading control. Densitometry analysis was performed and the Akt-P/Akt ratio was calculated. The results are the mean +/- SEM of three independent experiments. * p < 0.05, ** p < 0.01. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/19/7/2115) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0037.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT11 [p Ser473] antibody (17F6.B11). Lane 1: untreated NIH/3T3 cell lysates. Lane 2: detects phosphorylated AKT1 (indicated by arrowhead at ~56 kDa) on PDGF stimulated NIH/3T3 cell lysates. Load: 10 ug per lane.Primary antibody: AKT1 pS473 antibody at 1:10,000 in TBS with 0.05% Tween-20 with 1% BSA, for 1 h at 4 C.Secondary antibody: HRP conjugated Gt-a-Mouse IgG was used at a 1:20,000 dilution for 1 h at 4 C with FemtoMax(TM) enhanced chemiluminescent reagent.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0042.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT11 [p Ser473] antibody (17F6.B11). Lane 1: A431 cells. Lane 2: A431 cells stimulated for 15 min with EGF. Load: 35 ug per lane.Primary antibody: AKT1pS473 antibody at 1:400 for overnight at 4C.Secondary antibody: DyLight(TM)649 Conjugated Anti-AKT1 pS473 Monoclonal Antibody at 1:10,000 for 45 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting overnight at 4C.Predicted/Observed size: 56kDa. Other band(s): none.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0043.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT1 [p Ser473] antibody (17F6.B11). Lane 1: unstimulated NIH/3T3 lysates contain inactive unphosphorylated AKT11, green band. Lane 2: PDGF stimulated NIH/3T3 lysate contains both inactive (green band) and activated phosphorylated AKT11 (red band). Load: 10 ug per lane.Primary antibody: rabbit anti-AKT1 (pan) and mouse anti-AKT1 pS473 specific antibodies at 1:400 for overnight at 4C.Secondary antibody: DyLight(TM) 549 conjugated anti-rabbit IgG (green) and DyLight(TM) 649 conjugated anti-mouse IgG (red) secondary antibodies at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0044.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis of DyLight 488 conjugate of NBP1-69923. This image shows anti-histone detection using a DyLight 488 conjugate (green). Anti-Tubulin was detected using a DyLight 549 conjugate (red). Nuclei were counter-stained using DAPI (blue).](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0035.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923]](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0038.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Immunofluorescence Microscopy of AKT11 [p Ser473] antibody (17F6.B11) using STED nanoscopy to evaluate AKT1 activation and migration. Tissue: A431 cells. Antigen retrieval: Panel A: serum starved, unstimulated cells. Panel B: serum starved, EGF stimulated for 15 mins.A massive increase in AKT1-pS473 activation, as measured by intensity signal, peaked at 15 minutes and was associated with depolymerized tubulin.Staining: Panel A shows STED data (AKT1-pS473, red channel) collected simultaneously with confocal signal (a-tubulin, green channel). Upon stimulation of cells with EGF, a rapid activation of AKT1 is observed (Panel B) along with a coincident change in the tubulin organization (yellow signal), as well as an extensive cell shape-change (cell membrane folding) and accumulation of AKT1pS473 at the cell periphery.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0039.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis of Biotin conjugate of NBP1-69923. 20 ug/mL for 1 h at RT Secondary antibody: Streptavidin Conj. HRP 10 ug/ml Localization: nuclear and occasionally cytoplasmic Staining: antibody as precipitated red signal with a hematoxylin purple nuclear count](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunohistochemistry-Paraffin-NBP1-69923-img0034.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis of FFPE prostate tissue using AKT1 phospho Ser473 antibody (left panel). Negative control (right panel). Antigen retrieval with heat and pressure in citrate buffer pH 6.2. AKT1 phospho Ser473 antibody at 20 ug/mL. Secondary antibody Streptavidin-HRP at 10 ug/mL. Hematoxylin nuclear counterstain (purple). Image using the Biotin format of this antibody.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunohistochemistry-Paraffin-NBP1-69923-img0036.jpg)
![Western Blot of Mouse Anti-AKTpS473 antibody. Lane 1: A431 cells. Lane 2: A431 cells stimulated for 15 min with EGF. Load: 35 ug per lane. Primary antibody: AKTpS473 antibody at 1:400 for overnight at 4C. Secondary antibody: DyLight(TM)649 Conjugated Anti-AKT1 [p Ser473] Antibody (17F6.B11) at 1:10,000 for 45 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting overnight at 4C. Predicted/Observed size: 56kDa. Other band(s): none.](http://images.novusbio.com/fullsize/nbp1-69923_mouse-monoclonal-akt1-p-ser473-antibody-17f6-b11-255202313136.jpg)
![ELISA AKT1 [Biotin]](http://images.novusbio.com/fullsize2/dyc1775-2_human-mouse-rat-total-akt1-duoset-ic-elisa-2-plate-elisa-15920201506.jpg "ELISA AKT1 [Biotin]")
![Immunohistochemistry-Paraffin: AKT1 Antibody [NBP1-89159] - IHC analysis of human seminal vesicle and skeletal muscle tissues using anti-AKT1 antibody. Corresponding AKT1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/AKT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89159-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AKT1 Antibody [NBP1-89159] - IHC analysis of human seminal vesicle and skeletal muscle tissues using anti-AKT1 antibody. Corresponding AKT1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: AKT1 Antibody [NBP1-89159] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & microtubules.](http://images.novusbio.com/fullsize/AKT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89159-img0007.jpg)
![Immunohistochemistry-Paraffin: AKT1 Antibody [NBP1-89159] - IHC staining of human seminal vesicle shows high expression.](http://images.novusbio.com/fullsize/AKT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89159-img0008.jpg)
![Immunohistochemistry-Paraffin: AKT1 Antibody [NBP1-89159] - IHC staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/AKT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89159-img0009.jpg)
![Western Blot: AKT1 Antibody (PSH0-50) [NBP3-32016] - Western blot analysis of AKT1 on different lysates with Rabbit anti-AKT1 antibody (NBP3-32016) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: MCF7 cell lysate Lane 3: HeLa cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: C2C12 cell lysate Lane 6: COS-1 cell lysate Lane 7: PC-12 cell lysate Lane 8: Mouse brain tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 56 kDa Observed band size: 60 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32016) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32016_rabbit-akt1-mab-psh0-50-27820241514178.jpg "Western Blot: AKT1 Antibody (PSH0-50) [NBP3-32016] - Western blot analysis of AKT1 on different lysates with Rabbit anti-AKT1 antibody (NBP3-32016) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: MCF7 cell lysate Lane 3: HeLa cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: C2C12 cell lysate Lane 6: COS-1 cell lysate Lane 7: PC-12 cell lysate Lane 8: Mouse brain tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 56 kDa Observed band size: 60 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32016) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: AKT1 Antibody (PSH0-50) [NBP3-32016] - Immunocytochemistry analysis of Jurkat cells labeling AKT1 with Rabbit anti-AKT1 antibody (NBP3-32016) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-AKT1 antibody (NBP3-32016) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32016_rabbit-akt1-mab-psh0-50-2920241192386.jpg)
![Flow Cytometry: AKT1 Antibody (PSH0-50) [NBP3-32016] - Flow cytometric analysis of Jurkat cells labeling AKT1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32016, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32016_rabbit-akt1-mab-psh0-50-39202488468.jpg)
![Western Blot: AKT1 Antibody (CL0887) [NBP2-52956] - WB using AKT1 antibody. Lane 1: Marker [kDa], Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/AKT1-Antibody-CL0887-Western-Blot-NBP2-52956-img0001.jpg "Western Blot: AKT1 Antibody (CL0887) [NBP2-52956] - WB using AKT1 antibody. Lane 1: Marker [kDa], Lane 2: Human cell line RT-4")
![Immunocytochemistry/Immunofluorescence: AKT1 Antibody (CL0887) [NBP2-52956] - Staining of MCF7 cells using the Anti-AKT1 monoclonal antibody, showing specific staining in the nucleus, cytosol and plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/AKT1-Antibody-CL0887-Immunocytochemistry-Immunofluorescence-NBP2-52956-img0002.jpg)
![Western Blot: AKT1 [p Thr 450] Antibody (SR2048) [NBP3-21562] - Western blot analysis using NBP3-21562 in (1) 3T3 cell lysate treated with Lambda Phosphatase; (2) 3T3 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21562_rabbit-akt1-p-thr-450-mab-sr2048-57202316582143.jpg "Western Blot: AKT1 [p Thr 450] Antibody (SR2048) [NBP3-21562] - Western blot analysis using NBP3-21562 in (1) 3T3 cell lysate treated with Lambda Phosphatase; (2) 3T3 cell lysate.")