AIF Antibody (SZ05-01)

Western Blot: AIF Antibody (SZ05-01) [NBP2-67366] - Western blot analysis of AIF on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbi

Immunocytochemistry/Immunofluorescence: AIF Antibody (SZ05-01) [NBP2-67366] - Staining AIF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: AIF Antibody (SZ05-01) [NBP2-67366] - Analysis of paraffin-embedded human liver tissue using anti-AIF antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: AIF Antibody (SZ05-01) [NBP2-67366] - Analysis of Hela cells with AIF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: AIF Antibody (SZ05-01) [NBP2-67366] - Staining AIF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: AIF Antibody (SZ05-01) [NBP2-67366] - Analysis of paraffin-embedded human kidney tissue using anti-AIF antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: AIF Antibody (SZ05-01) [NBP2-67366] - Analysis of paraffin-embedded mouse heart tissue using anti-AIF antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: AIF Antibody (SZ05-01) [NBP2-67366] - Analysis of paraffin-embedded mouse liver tissue using anti-AIF antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: AIF Antibody (SZ05-01) [NBP2-67366] - Analysis of paraffin-embedded human kidney tissue using anti-AIF antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

AIF Antibody (SZ05-01) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human AIF aa 502-551 / 613. (SwissProt: O95831 Human; SwissProt: Q9Z0X1 Mouse; SwissProt: Q9JM53 Rat)
• Localization: Mitochondrion intermembrane space, Mitochondrion inner membrane, Cytoplasm, Nucleus, Membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: AIFM1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:1000
  • Immunoprecipitation
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for AIF Antibody (SZ05-01)

• AIF
• AIFapoptosis-inducing factor 1, mitochondrial
• AIFM1
• apoptosis-inducing factor, mitochondrion-associated, 1
• COXPD6
• MGC111425
• PDCD8
• PDCD8striatal apoptosis-inducing factor
• programmed cell death 8 (apoptosis-inducing factor)
• Programmed cell death protein 8

Background

AIF, or apoptosis-inducing factor, is a flavoprotein localized to the mitochondrial membrane that plays a primary role in caspase-independent cell death, or apoptosis (1,2). AIF translocates to the nucleus when apoptosis is induced and causes mitochondria to release the apoptogenic proteins, cytochrome c and caspase-9. Apoptotic activity of AIF leads to chromatin condensation and DNA fragmentation (1). Similar to cytochrome c, AIF is a bifunctional protein with an oxidoreductase function and a second apoptogenic function. In contrast to cytochrome c, however, AIF acts in a caspase-independent fashion (2,3,4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AIFM1