AIBZIP Antibody

Western Blot: AIBZIP Antibody [NB100-1015] - Staining (0.5ug/ml) of Human Placenta lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Immunohistochemistry-Paraffin: AIBZIP Antibody [NB100-1015] - IHC analysis of AIBZIP in paraffin embedded Human Prostate using this antibody at 2.5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

AIBZIP Antibody Summary

• Immunogen: Peptide with sequence C-PSGRIRSVLHADEM corresponding to C-Terminus according to NP_570968.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CREB3L4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5 - 2 ug/ml
• Application Notes: WB: Approx. 50 kDa band observed in human placenta lysate (predicted MW of 45 kDa band according to NP_570968). IHC-P: Human prostate shows strong staining of cytoplasm in the secretory cells of the gland.
• Control:

  Human Placenta Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AIBZIP Antibody

• AIbZIP
• AIBZIPcAMP-responsive element-binding protein 4
• Androgen-induced basic leucine zipper protein
• ATCE1JAL
• Attaching to CRE-like 1
• cAMP responsive element binding protein 1
• cAMP responsive element binding protein 3-like 4
• CREB3
• CREB4CREB-4
• cyclic AMP-responsive element-binding protein 3-like protein 4
• Cyclic AMP-responsive element-binding protein 4
• hJALcAMP-responsive element-binding protein 3-like protein 4
• tisp40
• Transcript induced in spermiogenesis protein 40

Background

Androgen-induced basic leucine zipper (AIBZIP)is a transcription factor. AIbZIP is a 395 aa protein with homology to cyclic AMP-responsive element binding protein/activating transcription factor. It contains an NH(2)-terminal activation domain, a central bZIP domain, and a COOH-terminal transmembrane domain. Immunoreactive AIbZIP protein has been detected in the cytoplasm of prostatic luminal epithelial cells; full-length AIbZIP-GFP fusion proteins are localized in the cytoplasm of LNCaP cells, while a truncated form of AIbZIP lacking the putative transmembrane domain was exclusively nuclear. AIbZIP is expressed at higher levels in cancerous prostate cells compared with noncancerous prostate cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AIBZIP Antibody (NB100-1015)(1)

Publication using NB100-1015

• Qi H, Fillion C, Labrie Y et al. AIbZIP, a novel bZIP gene located on chromosome 1q21.3 that is highly expressed in prostate tumors and of which the expression is up-regulated by androgens in LNCaP human prostate cancer cells. Cancer Res 2002-02-01 [PMID: 11830526]

Bioinformatics

• Gene Symbol: CREB3L4
• Entrez:
  • 148327(Human)
• Uniprot:
  • Q8TEY5(Human)