AIBZIP Antibody

Images

 
Western Blot: AIBZIP Antibody [NB100-1015] - Staining (0.5ug/ml) of Human Placenta lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry-Paraffin: AIBZIP Antibody [NB100-1015] - IHC analysis of AIBZIP in paraffin embedded Human Prostate using this antibody at 2.5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

AIBZIP Antibody Summary

Immunogen
Peptide with sequence C-PSGRIRSVLHADEM corresponding to C-Terminus according to NP_570968.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CREB3L4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 2 ug/ml
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 50 kDa band observed in human placenta lysate (predicted MW of 45 kDa band according to NP_570968). IHC-P: Human prostate shows strong staining of cytoplasm in the secretory cells of the gland.
Positive Control
Placenta Lysate (NB820-59248)
Publications
Read Publication using NB100-1015.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AIBZIP Antibody

  • AIbZIP
  • AIBZIPcAMP-responsive element-binding protein 4
  • Androgen-induced basic leucine zipper protein
  • ATCE1JAL
  • Attaching to CRE-like 1
  • cAMP responsive element binding protein 1
  • cAMP responsive element binding protein 3-like 4
  • CREB3
  • CREB4CREB-4
  • cyclic AMP-responsive element-binding protein 3-like protein 4
  • Cyclic AMP-responsive element-binding protein 4
  • hJALcAMP-responsive element-binding protein 3-like protein 4
  • tisp40
  • Transcript induced in spermiogenesis protein 40

Background

Androgen-induced basic leucine zipper (AIBZIP)is a transcription factor. AIbZIP is a 395 aa protein with homology to cyclic AMP-responsive element binding protein/activating transcription factor. It contains an NH(2)-terminal activation domain, a central bZIP domain, and a COOH-terminal transmembrane domain. Immunoreactive AIbZIP protein has been detected in the cytoplasm of prostatic luminal epithelial cells; full-length AIbZIP-GFP fusion proteins are localized in the cytoplasm of LNCaP cells, while a truncated form of AIbZIP lacking the putative transmembrane domain was exclusively nuclear. AIbZIP is expressed at higher levels in cancerous prostate cells compared with noncancerous prostate cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AIBZIP Antibody (NB100-1015)(1)

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CREB3L4
Entrez
Uniprot