AGPAT6 Antibody

Western Blot: AGPAT6 Antibody [NB100-2390] - Detection of AGPAT6 in mouse skeletal muscle using NB 100-2390.

Product Discontinued

AGPAT6 Antibody Summary

• Immunogen: A synthetic peptide made to a C-terminal portion of human AGPAT6 (between residues 400-456). [Swiss-Prot Q86UL3]
• Localization: Membrane
• Predicted Species: Rat (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AGPAT6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/ml (ECL)
• Application Notes: This AGPAT6 antibody is useful for Western blot, where a band is seen at ~58 kDa representing AGPAT6 protein. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 58 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Mouse Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Human, monkey and mouse. Predicted to react with rat, bovine, and porcine based on 100% sequence homology.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AGPAT6 Antibody

• 1-acylglycerol-3-phosphate O-acyltransferase 6 (lysophosphatidic acidacyltransferase, zeta)
• Acyl-CoA:glycerol-3-phosphate acyltransferase 4
• DKFZp586M1819,1-acyl-sn-glycerol-3-phosphate acyltransferase zeta
• EC 2.3.1.15
• glycerol-3-phosphate acyltransferase 4,1-AGP acyltransferase 6
• glycerol-3-phosphate acyltransferase 6,1-AGPAT 6
• GPAT4,1-acylglycerol-3-phosphate O-acyltransferase 6
• LPAAT-zetaLPAATZ
• Lysophosphatidic acid acyltransferase zeta
• testis spermatogenesis apoptosis-related protein 7
• TSARG7

Background

Member of 1-acylglycerol-3-phosphate-O-acyltransferase (AGPATs) family, AGPAT6 (glycerol-3-phosphate acyltransferase 4) is an enzyme essential for biosynthesis of glycerolipids and triacylglycerol in eukaryotes, as well as for catalyzing the conversion of lysophosphatidic acid to phosphatidic acid. It esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, which is a required step in glycerolipid biosynthesis. AGPAT6 is active against both saturated as well as unsaturated long-chain fatty acyl-CoAs and is inhibited by N-ethylmaleimide (NEM). Its HXXXXD motif is critical for its acyltransferase activity and constitutes the site for binding of glycerol-3-phosphate's phosphate moiety. Localized in ER membrane as multi-pass membrane protein, AGPAT6 is ubiquitously expressed with relatively high expression levels in skeletal muscle, heart, mammary epithelium and testis. AGPAT6 contributes to the production of milk fat and has been considered a candidate gene for regulating milk composition. Mouse model deficient in AGPAT6 exhibit weight loss and resistance to both diet-induced as well as genetically induced obesity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AGPAT6 Antibody (NB100-2390)(2)

Publications using NB100-2390

• Soltysik K, Ohsaki Y, Tatematsu T et al. Nuclear lipid droplets form in the inner nuclear membrane in a seipin-independent manner The Journal of cell biology 2021-01-04 [PMID: 33315072]
• Helsley RN, Varadharajan V, Brown AL et al. Obesity-linked suppression of membrane-bound O-acyltransferase 7 (MBOAT7) drives non-alcoholic fatty liver disease Elife 2019-10-17 [PMID: 31621579] (Mouse)

Review for AGPAT6 Antibody (NB100-2390) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Zachary Patinkin Simple Western Mouse 07/22/2015

Summary

• Application: Simple Western
• Sample Tested: Mouse skeletal muscle homogenate
• Species: Mouse
• Lot: A1

Bioinformatics

• Gene Symbol: AGPAT6