Ago2/eIF2C2 Antibody - BSA Free

Product Discontinued

Ago2/eIF2C2 Antibody - BSA Free Summary

• Immunogen: Amino acids 361-378 (TSTMIRATARSAPDRQEE) of human Argonaut 2 protein were used as the immunogen. Genbank accession number: NP_036286.
• Specificity: The amino acid sequence used is 100% homologous in human Argonaut 2 (Gene ID: 27161, UniProt: Q9UKV8), Argonaut 3 (Gene ID: 192669, UniProt: Q9H9G7), and Argonaut 4 (Gene ID: 192670, UniProt: Q9HCK5) and 94% homologous in Argonaut 1. The immunizing sequence is highly conserved between various isoforms (isoforms 1, 2, 3 and 4, some having yet additional isoforms).
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Rabbit (100%), Zebrafish (94%), Avian (100%), Fish (94%), Canine (94%), Xenopus (100%), Chinese Hamster (100%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AGO2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Electron Microscopy
  • Immunoprecipitation reported in scientific literature (PMID 29467316)
  • Peptide ELISA 1:100-1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera with 50% Glycerol
• Preservative: 0.025% Sodium Azide
• Purity: Unpurified

Alternate Names for Ago2/eIF2C2 Antibody - BSA Free

• AGO2argonaute 2
• argonaute2
• EC 3.1.26.n1
• EC 3.1.26.n2
• eIF2C 2
• eIF-2C 2
• Eukaryotic translation initiation factor 2C 2
• eukaryotic translation initiation factor 2C, 2
• hAgo2
• MGC3183
• PAZ Piwi domain protein
• PPD
• protein argonaute-2
• Protein slicer
• Q10

Background

Small interfering RNAs (siRNAs) have gained much attention for their powerful ability to suppress gene expression. Introduction of double-stranded RNA (dsRNA), that are homologues in sequence to a gene, has proven to suppress that gene's expression through a process known as RNA interference (RNAi); this process post-transcriptionally silences a gene through mRNA inhibition or degradation. Both biochemical and genetic studies have led to the identification of two, conserved gene families that are universal components of the interference process. These are the Dicer family, which is comprised of members of the Ribonuclease III family of enzymes, and the Argonaute gene family. Argonaute proteins addition to their role in RNAi process, may also influence development, such as, stem cell fate determination.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ago2/eIF2C2 Antibody (NB100-56198)(2)

Publications using NB100-56198

• Reed JC, Westergreen N, Barajas BC, Ressler DTB. The formation of RNA granule-derived capsid assembly intermediates appears to be conserved between HIV-1 and the non-primate lentivirus FIV. J. Virol. 2018-02-21 [PMID: 29467316] (IP)
• Jaglarz MK, Kloc M, Jankowska W et al. Nuage morphogenesis becomes more complex: two translocation pathways and two forms of nuage coexist in Drosophila germline syncytia. Cell Tissue Res. 2011-04-01 [PMID: 21365220]

FAQs for Ago2/eIF2C2 Antibody (NB100-56198). (Showing 1 - 1 of 1 FAQs).

1. I am interested in the Ago2/3/4 Antibody (NB100-56198), but I am working with bobtail squid an I want to check if the antibody will work. This is the nucleotid sequence of the AGO gene in the squid: AACATGTTCGGGGCCACTAGCATATTTACTAAAAACAAGGCTGCCCACAGATGGGCATTCCTGCATCGTTAGATATCCGCTGCAGTTGTTGAGTGAAGCTCCGAAGAGCCTCTTCCTTGACGGACTTCTGAGCAGCAAAACAAGCAATGGCCCATACTCGGATCTCAATTCCATTATAAAACTGTTTGCCTCGCATATCCCAAACACCTTGATTAGGTATCGCTAAGGCTTTCGTTCGACCACCATATTGGATTTTTGGGGCATCAATGACACGCCCCTCAACCTCAACCATTTTGGGATTAACTCTGACCCCAAACTGAAATAGGTATGGATCTGTACCAAAGTTTGCTTTAGATACCAAACTGTTAATATCCCGTTCTCTGTCTGGAGCTGAACGAGCTGTTGCCTTGATCATTGTCGATGTTTGCATATCGGTTAATTTCTTGATACAACGTTGCCCTCCTACAATGTTGCATACTTCTAATGGGAGGTAGGTATGTTTCTGTTCTTGTCCAACTTGAAGACAGGGTAAATATGGATACAGTAATCTAATTTTATATCTCTCAATAAAAATATCTTGCAACTGTACACTCAACTGTTTGGCCACTTTCTAATTGGAGAGGGAAAGACTGGGTTTGAGCAGGTCGTCTGGTCACGTTACAAACTCTGTA. I have the protein sequence: KVAKQLSVQLQDIFIERYKIRLLYPYLPCLQVGQEQKHTYLPLEVCNIVGGQRCIKKLTDMQTSTMIKATARSAPDRERDINSLVSKANFGTDPYLFQFGVRVNPKMVEVEGRVIDAPKIQYGGRTKALAIPNQGVWDMRGKQFYNGIEIRVWAIACFAAQKSVKEEALRSFTQQLQRISNDAGMPICGQPCF
   • The immunogen for NB100-56198 shares 88% similarity with your sequence, so it is expected to cross-react.

Bioinformatics

• Gene Symbol: AGO2