EIF2C2 (AAH07633, 1 a.a. ~ 377 a.a) full-length human protein with GST tag.MPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVD
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Ago2/eIF2C2 Antibody
Eukaryotic translation initiation factor 2C 2
eukaryotic translation initiation factor 2C, 2
PAZ Piwi domain protein
This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. [provided by RefSeq]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
recombinant protein (sold by Novus) and total exosome lysate
5% blocking power in PBS + 0.05% Tween 20; 1h RT
1:500 dil of antibody in blocking buffer (see above); 4C, overnight
anti-mouse goat HRP
Secondary Manufacturer Cat#
1/1000 dil in blocking buffer, 1h RT
SuperSignal West Dura Extended Duration Substrate (Thermo, ref# 34075) for developing blot; ECL film and developer
There was good recognition of the recombinant protein (previous antibody NB100-56198 did not detect it) for 10ng of loaded protein; 50ng of loaded protein showed more non-specific signal, especially at higher molecular wight than the expected size of 64kDa.The antibody was also tested in 4 different samples (1.25 x 10^9 exosomes/lane) showing a clean protein profile. At this point, it is unclear if the protein detected >50kDa is a non-specific band or a related protein of lower molecular weight.
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