Ago2/eIF2C2 Antibody

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Western Blot: Ago2/eIF2C2 Antibody [H00027161-A01] - Detection against Immunogen (68.4 KDa).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Ago2/eIF2C2 Antibody Summary

Immunogen
EIF2C2 (AAH07633, 1 a.a. ~ 377 a.a) full-length human protein with GST tag.MPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVD
Specificity
EIF2C2 - eukaryotic translation initiation factor 2C, 2
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
EIF2C2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Reviewed Applications
Read 1 Review rated 4
using
H00027161-A01 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
50% Glycerol
Preservative
No Preservative
Purity
Unpurified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Ago2/eIF2C2 Antibody

  • AGO2argonaute 2
  • argonaute2
  • EC 3.1.26.n1
  • EC 3.1.26.n2
  • eIF2C 2
  • eIF-2C 2
  • Eukaryotic translation initiation factor 2C 2
  • eukaryotic translation initiation factor 2C, 2
  • hAgo2
  • MGC3183
  • PAZ Piwi domain protein
  • PPD
  • protein argonaute-2
  • Protein slicer
  • Q10

Background

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ago2/eIF2C2 Antibody (H00027161-A01) (0)

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Review for Ago2/eIF2C2 Antibody (H00027161-A01) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using H00027161-A01:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot Ago2/eIF2C2 H00027161-A01
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reviewed by:
Anonymous
WB Human 12/15/2015
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Summary

ApplicationWestern Blot
Sample Testedrecombinant protein (sold by Novus) and total exosome lysate
SpeciesHuman
Lot09351

Blocking

Blocking Details5% blocking power in PBS + 0.05% Tween 20; 1h RT

Primary Anitbody

Dilution Ratio1:500 dil of antibody in blocking buffer (see above); 4C, overnight

Secondary Antibody

Secondary Descriptionanti-mouse goat HRP
Secondary Manufacturer Cat#Invitrogen M30107
Secondary Concentration1/1000 dil in blocking buffer, 1h RT

Details

Detection NotesSuperSignal West Dura Extended Duration Substrate (Thermo, ref# 34075) for developing blot; ECL film and developer

Comments

CommentsThere was good recognition of the recombinant protein (previous antibody NB100-56198 did not detect it) for 10ng of loaded protein; 50ng of loaded protein showed more non-specific signal, especially at higher molecular wight than the expected size of 64kDa.The antibody was also tested in 4 different samples (1.25 x 10^9 exosomes/lane) showing a clean protein profile. At this point, it is unclear if the protein detected >50kDa is a non-specific band or a related protein of lower molecular weight.

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FAQs for Ago2/eIF2C2 Antibody (H00027161-A01) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for Ago2/eIF2C2 Antibody (H00027161-A01)

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Diseases for Ago2/eIF2C2 Antibody (H00027161-A01)

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Blogs on Ago2/eIF2C2.

Ago2 antibodies and dicer-independent biogenesis of miRNA
Ago2, also called eIF2C2, antibody is one of 37 reagents targeted to the Argonaute protein family that we at Novus Biologicals have in our antibody catalogue. Argonaute proteins are encoded by genes which play an important role in regulating the contr...  Read full blog post.

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Recent Reviews

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Anonymous
12/15/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol EIF2C2
Entrez
Uniprot