Ago2/eIF2C2 Antibody (11A9) - BSA Free

Western Blot: Ago2/eIF2C2 Antibody (11A9) [NBP3-23228] - Western blot analysis of human A431 cells treated with EGF (100 ng/ml for 60 min.) (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4) then probed with rat monoclonal anti-Ago2/eIF2C2 (NBP3-23228) (lanes 1 & 2) and rabbit polyclonal anti-Ago2/eIF2C2 (Tyr-393) phospho-specific antibody (lanes 3 & 4).

• Reactivity: Hu, Bv
• Applications: WB
• Clone: 11A9
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free

Ago2/eIF2C2 Antibody (11A9) - BSA Free Summary

• Description: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).
• Immunogen: Clone 11A9 was generated from an Ago2/eIF2C2 synthetic peptide (coupled to KLH) corresponding to amino acids in the N-terminus of human Ago2/eIF2C2. This sequence has low homology to other argonaute family members. [UniProt# Q9UKV8]
• Specificity: The antibody detects a 97 kDa protein corresponding to the apparent molecular mass ofAgo2/eIF2C2 on SDS-PAGE immunoblots of several human cell lines, A431, HeLa, MCF-7, and K562. The antibody does not detect rat or mouse Ago2/eIF2C2, but will detect bovine Ago2/eIF2C2.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: AGO2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:250
• Theoretical MW: 97 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for Ago2/eIF2C2 Antibody (11A9) - BSA Free

• AGO2argonaute 2
• argonaute2
• EC 3.1.26.n1
• EC 3.1.26.n2
• eIF2C 2
• eIF-2C 2
• Eukaryotic translation initiation factor 2C 2
• eukaryotic translation initiation factor 2C, 2
• hAgo2
• MGC3183
• PAZ Piwi domain protein
• PPD
• protein argonaute-2
• Protein slicer
• Q10

Background

Ago2 / eIF2C2 encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AGO2