Aggrecan Antibody (Cat-315)

Immunocytochemistry/Immunofluorescence: Aggrecan Antibody (Cat-315) [NBP2-29796] - Mouse anti-CSPG staining of primary cortical culture cells from rat.

• Reactivity: Rt, Fe
• Applications: WB, ICC/IF, IHC, IP
• Clone: Cat-315
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Aggrecan Antibody (Cat-315) Summary

• Description: Novus Biologicals Mouse Aggrecan Antibody (Cat-315) (NBP2-29796) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Feline brain proteoglycans.
• Marker: Chondrocyte Marker
• Specificity: Chondroitin sulfate proteoglycan (CSPG), protein core epitope.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACAN
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Immunohistochemistry 1:2000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:5000
• Application Notes: Immunohistochemistry: on 4% paraformaldehyde fixed frozen tissue. Immunocytochemistry/Immunofluorescence :Primary cultures of neurons. ImmunoBlot: recognizes a band at 680 kDa.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for Aggrecan Antibody (Cat-315)

• ACAN
• AGC1
• AGC1SEDK
• aggrecan core protein
• Aggrecan
• Cartilage-specific proteoglycan core protein
• Chondroitin sulfate proteoglycan 1
• Chondroitin sulfate proteoglycan core protein 1
• CSPCP
• CSPG1
• CSPG1aggrecan 1
• CSPGCP
• large aggregating proteoglycan
• MSK16
• MSK16AGCAN
• SEDK

Background

This aggrecan degradation product usually remains within the tissue still complexed to hyaluronan and link protein. Its release from the cartilage usually signals that there has been extensive catabolism of aggrecan, which allows large complexes containing this metabolite to be released from the tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACAN
• Uniprot:
  • P16112()