Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Tissue culture supernatant
Western Blot 1:100
This Aggrecan antibody is useful in Immunohistochemistry, ELISA and Western blot. With IHC the antibody works in formalin- or paraformaldehyde-fixed paraffin embedded sections as well as either alcohol-fixed frozen sections or un-fixed snap-frozen sections. In Western blot the antibody detects a variety of epitopes between 50 and 250 kDa. Samples must be deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01 Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10ug S-GAG of non-deglycosylated aggrecan for optimal epitope recognition. See Little et al. and Caterson et al. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27185069).
250 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Cross-reacts with Human, Cow, Cat, Dog, Guinea pig, Horse, Pig, Rabbit, Rat and Sheep. Does not react with Mouse. Not yet tested in other species.
Alternate Names for Aggrecan Antibody (BC-3)
aggrecan core protein
Cartilage-specific proteoglycan core protein
Chondroitin sulfate proteoglycan 1
Chondroitin sulfate proteoglycan core protein 1
large aggregating proteoglycan
The Aggrecan degradation product usually remains within the tissue still complexed to hyaluronan and link protein. Its release from the cartilage usually signals that there has been extensive catabolism of aggrecan, which allows large complexes containing this metabolite to be released from the tissue. Aggrecan degradation products can be found in the synovial fluids of patients with degenerative joint disease.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Aggrecan Antibody (NB600-504). (Showing 1 - 3 of 3 FAQs).
Our customer would like to confirm the predicted MW by using NB600-504 to detect Aggrecan. According to info from http://www.uniprot.org/uniprot/P16112, the MW of the target protein may be between 239 to 250 kDa. But this antibody can also be used with ELISA and Western blot, where it detects a variety of epitopes between 50 and 250 kDa is described. May we have the explanation?
There are two isoforms of aggrecan, and this antibody can detect either one or both of them. It depends on which isoforms a particular cell type/tissue/species expresses.
Do you know if this antibody has been used with immunofluorescence?
We have not yet validated this antibody for use in immunofluorescence. In instances such as this, we recommend our Innovators Reward Program. Under the terms and conditions of this program, we would be happy to provide you with a credit of equal or lesser value, in exchange for your online review of this product including an image of either positive or negative staining for testing in a new species and/or application.
On behalf of a customer I have a question about antibody NB600-504 anti-ACAN. The note on the datasheet says Samples must be deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01 Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10ug S-GAG of non-deglycosylated aggrecan for optimal epitope recognition. Do you really mean to say deglycosylated? Are not those reagents actually digesting the aggrecan? Do they also deglycosylate the protein?
Aggrecan is a highly glycosylated protein and this can interfere with the antibody binding to and recognizing the epitope. These enzymes will deglycosylate the protein and expose the epitope for recognition by the antibody