Aggrecan Antibody (BC-3)

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe, GP, Rb, ShSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
BC-3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Aggrecan Antibody (BC-3) Summary

Immunogen
ARGSV... synthetic peptide conjugate.
Localization
Secreted
Marker
Chondrocyte Marker
Specificity
Recognizes the aggrecanase (ADAMTS-1, -4 & -5)-generated N-terminal neoepitope ARG after cleavage between amino acids EGE and ARG within the interglobular domain of aggrecanase-catabolised aggrecan.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ACAN
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This Aggrecan antibody is useful in Immunohistochemistry, ELISA and Western blot. With IHC the antibody works in formalin- or paraformaldehyde-fixed paraffin embedded sections as well as either alcohol-fixed frozen sections or un-fixed snap-frozen sections. In Western blot the antibody detects a variety of epitopes between 50 and 250 kDa. Samples must be deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01 Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10ug S-GAG of non-deglycosylated aggrecan for optimal epitope recognition. See Little et al. and Caterson et al. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27185069).
Theoretical MW
250 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB600-504 in the following applications:

  • IHC
    3 publications
  • 1 publication
  • WB
    1 publication

Reactivity Notes

Cross-reacts with Human, Cow, Cat, Dog, Guinea pig, Horse, Pig, Rabbit, Rat and Sheep. Mouse reactivity reported in scientific literature (PMID: 27185069). Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
0.02% Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for Aggrecan Antibody (BC-3)

  • ACAN
  • AGC1
  • AGC1SEDK
  • aggrecan core protein
  • Aggrecan
  • Cartilage-specific proteoglycan core protein
  • Chondroitin sulfate proteoglycan 1
  • Chondroitin sulfate proteoglycan core protein 1
  • CSPCP
  • CSPG1
  • CSPG1aggrecan 1
  • CSPGCP
  • large aggregating proteoglycan
  • MSK16
  • MSK16AGCAN
  • SEDK

Background

The Aggrecan degradation product usually remains within the tissue still complexed to hyaluronan and link protein. Its release from the cartilage usually signals that there has been extensive catabolism of aggrecan, which allows large complexes containing this metabolite to be released from the tissue. Aggrecan degradation products can be found in the synovial fluids of patients with degenerative joint disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB600-504
Species: Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe, GP, Rb, Sh
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for Aggrecan Antibody (NB600-504)(7)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, Rabbit.

We have publications tested in 3 applications: IHC, IHC-P, WB.


Filter By Application
IHC
(3)
IHC-P
(1)
WB
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(2)
Rat
(2)
Bovine
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB600-504 Applications Species
Han M, Kang BJ, Kim S et al. Extracellular Matrix-based Cryogels for Cartilage Tissue Engineering. Int. J. Biol. Macromol. Oct 10 2016 [PMID: 27185069] (IHC, Mouse) IHC Mouse
Han M, Kang BJ, Kim S et al. Extracellular Matrix-based Cryogels for Cartilage Tissue Engineering. Int. J. Biol. Macromol. Oct 10 2016 [PMID: 27185069] (IHC, Mouse) IHC Mouse
Bouhenni R, Hart M, Al-Jastaneiah S et al. Immunohistochemical expression and distribution of proteoglycans and collagens in sclerocornea. Int Ophthalmol. 2013 Jan [PMID: 23325424] (IHC-P, Human) IHC-P Human
Yurube T, Takada T, Suzuki T, Kakutani K, Maeno K, Doita M, Kurosaka M, Nishida K. Rat tail static compression model mimics extracellular matrix metabolic imbalances of matrix metalloproteinases, aggrecanases, and tissue inhibitors of metalloproteinases in intervertebral disc degeneration. Arthritis Res Ther;14(2):R51. 2012 Mar 6. [PMID: 22394620] (IHC, Rat) IHC Rat
Swearingen CA, Chambers MG, Lin C et al. A short-term pharmacodynamic model for monitoring aggrecanase activity: injection of monosodium iodoacetate (MIA) in rats and assessment of aggrecan neoepitope release in synovial fluid using novel ELISAs. Osteoarthritis Cartilage 2010 Sep [PMID: 20633676] (WB, Bovine, Human, Rat) WB Bovine, Human, Rat
Little CB, Hughes CE, Curtis CL, Janusz MJ, Bohne R, Wang-WeigS, Taiwo YO, Mitchell PG, Otterness IG, Flannery CR, Caterson B. Matrix metalloproteinases are involved in C-terminal and interglobular domain processing of cartilage aggrecan in late stage cartilage degradation. Matrix Biol;21(3):271-88. 2002 Apr. [PMID: 12009333]
Ma S, Xie Z, Yan G et al. Effect of Acupotomy on FAK-PI3K signaling pathways in KOA rabbit articular cartilages Evidence-Based Complementary and Alternative Medicine 2017 Aug 28 (Rabbit) Rabbit

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Product General Protocols

View specific protocols for Aggrecan Antibody (NB600-504): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Aggrecan Antibody (NB600-504). (Showing 1 - 3 of 3 FAQs).

  1. Our customer would like to confirm the predicted MW by using NB600-504 to detect Aggrecan. According to info from http://www.uniprot.org/uniprot/P16112, the MW of the target protein may be between 239 to 250 kDa. But this antibody can also be used with ELISA and Western blot, where it detects a variety of epitopes between 50 and 250 kDa is described. May we have the explanation?
    • There are two isoforms of aggrecan, and this antibody can detect either one or both of them. It depends on which isoforms a particular cell type/tissue/species expresses.
  2. Do you know if this antibody has been used with immunofluorescence?
    • We have not yet validated this antibody for use in immunofluorescence. In instances such as this, we recommend our Innovators Reward Program. Under the terms and conditions of this program, we would be happy to provide you with a credit of equal or lesser value, in exchange for your online review of this product including an image of either positive or negative staining for testing in a new species and/or application.
  3. On behalf of a customer I have a question about antibody NB600-504 anti-ACAN. The note on the datasheet says Samples must be deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01 Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10ug S-GAG of non-deglycosylated aggrecan for optimal epitope recognition. Do you really mean to say deglycosylated? Are not those reagents actually digesting the aggrecan? Do they also deglycosylate the protein?
    • Aggrecan is a highly glycosylated protein and this can interfere with the antibody binding to and recognizing the epitope. These enzymes will deglycosylate the protein and expose the epitope for recognition by the antibody

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Aggrecan Antibody (NB600-504)

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Blogs on Aggrecan.

Aches & Pains: Aggrecan in Joint Disease and Osteoarthritis
Aggrecan is essential for the normal function of articular cartilage and intervertebral discs. Aggrecan provides the ability for the tissues to withstand compressive loading. This property is dependent on both the high charge density endowed by its nu...  Read full blog post.

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Bioinformatics

Gene Symbol ACAN
Entrez
Uniprot