Afadin/AF-6 Antibody (3)

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA, IHC, IP
• Clone: 3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

Afadin/AF-6 Antibody (3) Summary

• Description: Novus Biologicals Mouse Afadin/AF-6 Antibody (3) (NBP3-11187) is a monoclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Monoclonal antibody 3 has been raised against peptide 1814-1829 aa of rat l-afadin.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: AFDN
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunoassay
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
  • Western Blot
• Application Notes: For Western blot and Immunohistochemistry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Fixation with PFA or acetone/methanol is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 0.2 um filtered solution in PBS, 0.1% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for Afadin/AF-6 Antibody (3)

• AF6
• AF-6
• AF64
• AFAD
• Afadin
• FLJ34371
• MLLT4
• myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 4

Background

Afadin is an actin filament-binding protein that is involved in cell-cell adherens junctions (AJs) which directly interacts with the alpha herpus virus receptor, a member of the immunoglobulin supergene family. Afadin is colocalized with nectin at cadherin-based cell-cell adherence junctions in various tissues and cell lines. L-Afadin has been isolated as an F-actin-binding protein from rat brain. L-afadin is ubiquitously expressed and specifically localized at zonula adherens (ZA) in epithelial cells and at cell-cell adherens junctions (AJs) in non-epithelial cells. L-afadin has a splice variant, named small intestine absorptive afadin (s-afadin) which is abundantly expressed in neural tissue. Monoclonal antibody 3 has been raised against peptide 1814-1829 aa of rat l-afadin. Monoclonal antibody 3 cross reacts with human, mouse and dog l-afadin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AFDN