![Immunohistochemistry-Paraffin: AE2 Antibody [NBP3-14535] - Immunohistochemistry of formalin-fixed, paraffin-embedded human kidney after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/AE2-Antibody-Immunohistochemistry-Paraffin-NBP3-14535-img0001.jpg "Immunohistochemistry-Paraffin: AE2 Antibody [NBP3-14535] - Immunohistochemistry of formalin-fixed, paraffin-embedded human kidney after heat-induced antigen retrieval.")
| Reactivity | Hu, Pm, Pm, Pm-Or, PmSpecies Glossary |
| Applications | IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 0.11 mg/ml |
| Description | Product can be stored undiluted at 4C for up to 1 month. |
| Immunogen | Synthetic 16 amino acid peptide from internal region of human AE2. |
| Epitope | Internal |
| Specificity | Human AE2. BLAST analysis of the peptide immunogen showed no homology with other human proteins. |
| Predicted Species | Primate (100%), Monkey (100%), Orangutan (100%), Chimpanzee (100%). Backed by our 100% Guarantee. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SLC4A2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 10 ug/ml.. |
| Storage | Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.1% Sodium Azide |
| Concentration | 0.11 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for AE2 Antibody (NBP3-14535)Find related products by research area.
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| Gene Symbol | SLC4A2 |
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")