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SLC4A8 Antibody (6E11)

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Immunohistochemistry-Paraffin: SLC4A8 Antibody (6E11) [H00009498-M04] - Analysis of monoclonal antibody to SLC4A8 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml
Immunohistochemistry-Paraffin: SLC4A8 Antibody (6E11) [H00009498-M04] - Analysis of monoclonal antibody to SLC4A8 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml
ELISA: SLC4A8 Antibody (6E11) [H00009498-M04] - Detection limit for recombinant GST tagged SLC4A8 is 1 ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
6E11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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SLC4A8 Antibody (6E11) Summary

Immunogen
SLC4A8 (NP_004849, 187 a.a. ~ 286 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. IEEISDLILDQQELSSDLNDSMRVKVREALLKKHHHQNEKKRNNLIPIVRSFAEVGKKQSDPHLMDKHGQTVSPQSVPTTNLEVKNGVNCEHSPVDLSKV
Specificity
SLC4A8 - solute carrier family 4, sodium bicarbonate cotransporter, member 8
Isotype
IgG3 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SLC4A8
Purity
IgG purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Reviewed Applications
Read 1 Review rated 3
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H00009498-M04 in the following applications:
Publications
Read Publication using H00009498-M04.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SLC4A8 Antibody (6E11)

  • DKFZp761B2318
  • Electroneutral Na(+)-driven Cl-HCO3 exchanger
  • FLJ46462
  • KIAA0739
  • k-NBC3
  • Na+-driven Cl-HCO3 exchanger
  • NBC
  • NBC3electroneutral sodium bicarbonate exchanger 1
  • NDCBE
  • NDCBE1
  • Solute carrier family 4 member 8
  • solute carrier family 4, sodium bicarbonate cotransporter, member 8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: SLC4A4 Antibody [NBP2-32020] - Analysis in human kidney and lymph node tissues. Corresponding SLC4A4 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SLC4A4 Antibody [NBP2-32020] - Staining of human kidney shows strong cytoplasmic/ membranous positivity in cells in tubules.
Immunohistochemistry-Paraffin: SLC4A4 Antibody [NBP2-32020] - Staining of human lymph node shows no positivity as expected.
Species: Hu
Applications: IHC, IHC-P
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Immunohistochemistry-Paraffin: SLC4A7/Sodium bicarbonate cotransporter 3 Antibody [NBP1-87850] - Analysis in human duodenum and liver tissues. Corresponding SLC4A7 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SLC4A7/Sodium bicarbonate cotransporter 3 Antibody [NBP1-87850] - Staining of human tonsil shows moderate to strong membranous positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: SLC4A7/Sodium bicarbonate cotransporter 3 Antibody [NBP1-87850] - Staining of human liver shows no positivity in hepatocytes.
Species: Hu
Applications: IHC, IHC-P
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Immunocytochemistry/Immunofluorescence: NHE1/SLC9A1 Antibody [NBP2-38683] - Staining of human cell line A-431 shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NHE1/SLC9A1 Antibody [NBP2-38683] - Staining of human kidney shows moderate membranous/cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: NHE1/SLC9A1 Antibody [NBP2-38683] - Staining of human liver shows no positivity in hepatocytes as expected.
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Immunohistochemistry-Paraffin: SLC4A5 Antibody [NBP2-93493] - Paraffin-embedded human liver using SLC4A5 .
Immunohistochemistry-Paraffin: SLC4A5 Antibody [NBP2-93493] - Paraffin-embedded rat kidney using SLC4A5 .
Western Blot: SLC4A5 Antibody [NBP2-93493] - Analysis of extracts of various cell lines, using SLC4A5 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 15s.
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CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using 8 µg/mL Mouse Anti-Human CFTR C‑Terminus Monoclonal Antibody (Catalog # MAB25031) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(13 Reviews)
(45 Publications)
Species: Hu
Applications: IHC, IP, WB
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Immunohistochemistry-Paraffin: SLC4A10 Antibody [NBP1-82594] - Analysis in human cerebral cortex and lymph node tissues using NBP1-82594 antibody. Corresponding SLC4A10 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SLC4A10 Antibody [NBP1-82594] - Staining of human cerebral cortex shows moderate membranous positivity in neurons.
Immunohistochemistry-Paraffin: SLC4A10 Antibody [NBP1-82594] - Staining of human cerebellum shows strong membranous positivity in Purkinje cells.
Species: Hu
Applications: IHC, IHC-P
Carbonic Anhydrase
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Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Detection of Carbonic Anhydrase II in mouse embryonic whole cell lysate. Image provided via product review by verified customer.
Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - analysis of Carbonic Anhydrase II protein (loading amount: 50 ng) using rabbit anti-Carbonic Anhydrase II antibody (NB600-919) at a dilution of 1:1000, incubation - overnight at 4C.
Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Localization of CAII in mouse peritoneum. Image from verified customer review.
(3 Reviews)
(10 Publications)
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Immunohistochemistry-Paraffin: SLC26A4 Antibody [NBP1-85237] - Staining of human thyroid gland shows weak to moderate cytoplasmic and membranous positivity in follicle cells.
Immunohistochemistry-Frozen: SLC26A4 Antibody [NBP1-85237] - Human thyroid tissue was incubated with anti-SLC26A4 antibody at dilution 1:100 overnight at 4C and detected with Alexa Fluor 488 Donkey anti-rabbit at dilution 1:1000. Nuclei stained with DAPI. Image from verified customer review.
Immunohistochemistry-Paraffin: SLC26A4 Antibody [NBP1-85237] - Staining of human cervix shows no positivity in squamous epithelial cells as expected.
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A8/Angiotensinogen An ...
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(9 Publications)
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Immunohistochemistry: Band 3 Antibody [NBP2-49409] - Staining of human spleen shows strong positivity in erythrocytes.
Immunohistochemistry-Paraffin: Band 3 Antibody [NBP2-49409] - Staining of human bone marrow shows high expression.
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Applications: IHC, IHC-P
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Immunohistochemistry-Paraffin: SLCO6A1 Antibody [NBP2-49118] - Staining of human testis shows high expression.
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(182410) [Unconjugate ...
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Publications for SLC4A8 Antibody (H00009498-M04)(1)


Review for SLC4A8 Antibody (H00009498-M04) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Bovine.

Reviews using H00009498-M04:
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Western Blot SLC4A8 H00009498-M04
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reviewed by:
Benjamin Hung
 Western Blot Bovine 02/15/2017
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Summary

ApplicationWestern Blot
Sample TestedBCMEC: Bovine Cerebral Microvascular Endothelial Cells
SpeciesBovine
Lot08365-6E11

Comments

CommentsGel: 8% SDS-PAGE Gel

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Western Blot SLC4A8 H00009498-M04
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 Benjamin Hung
02/15/2017
Application: Western Blot
Species: Bovine
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Bioinformatics

Gene Symbol SLC4A8
Entrez
OMIM