![Immunohistochemistry-Paraffin: SLC4A8 Antibody (6E11) [H00009498-M04] - Analysis of monoclonal antibody to SLC4A8 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize2/SLC4A8-Antibody-(6E11)-Immunohistochemistry-Paraffin-H00009498-M04-img0002.jpg "Immunohistochemistry-Paraffin: SLC4A8 Antibody (6E11) [H00009498-M04] - Analysis of monoclonal antibody to SLC4A8 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC, IHC-P |
| Clone | 6E11 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | SLC4A8 (NP_004849, 187 a.a. ~ 287 a.a) partial recombinant protein with GST tag.IEEISDLILDQQELSSDLNDSMRVKVREALLKKHHHQNEKKRNNLIPIVRSFAEVGKKQSDPHLMDKHGQTVSPQSVPTTNLEVKNGVNCEHSPVDLSKV |
| Specificity | SLC4A8 - solute carrier family 4, sodium bicarbonate cotransporter, member 8 |
| Isotype | IgG3 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SLC4A8 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4) |
| Preservative | No Preservative |
| Purity | IgG purified |
| Publication using H00009498-M04 | Applications | Species |
|---|---|---|
| Kim Y, Trussell LO. Negative shift in the glycine reversal potential mediated by a Ca2+- and pH-dependent mechanism in interneurons. J Neurosci 29(37):11495-510. 2009 Sep 16. [PMID: 19759298] |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Anonymous |
WB | Bovine | 02/15/2017 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
Diseases for SLC4A8 Antibody (H00009498-M04)Discover more about diseases related to SLC4A8 Antibody (H00009498-M04).
| Pathways for SLC4A8 Antibody (H00009498-M04)View related products by pathway.
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PTMs for SLC4A8 Antibody (H00009498-M04)Learn more about PTMs related to SLC4A8 Antibody (H00009498-M04).
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![ELISA: SLC4A8 Antibody (6E11) [H00009498-M04] - Detection limit for recombinant GST tagged SLC4A8 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize2/SLC4A8-Antibody-(6E11)-ELISA-H00009498-M04-img0004.jpg "ELISA: SLC4A8 Antibody (6E11) [H00009498-M04] - Detection limit for recombinant GST tagged SLC4A8 is 1 ng/ml as a capture antibody.")
![Immunohistochemistry-Paraffin: SLC4A4 Antibody [NBP2-32020] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize2/SLC4A4-Antibody-Immunohistochemistry-Paraffin-NBP2-32020-img0007.jpg)
![Immunohistochemistry-Paraffin: SLC4A4 Antibody [NBP2-32020] - Staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize2/SLC4A4-Antibody-Immunohistochemistry-Paraffin-NBP2-32020-img0006.jpg)
![Immunohistochemistry-Paraffin: SLC4A4 Antibody [NBP2-32020] - Staining in human kidney and lymph node tissues using anti-SLC4A4 antibody. Corresponding SLC4A4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/SLC4A4 Antibody-Immunohistochemistry-Paraffin-NBP2-32020-img0008.jpg)
![Immunohistochemistry-Paraffin: SLC4A7/Sodium bicarbonate cotransporter 3 Antibody [NBP1-87850] - Staining of human duodenum shows moderate to strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize2/SLC4A7-Sodium-bicarbonate-cotransporter-3-Antibody-Immunohistochemistry-Paraffin-NBP1-87850-img0010.jpg)
![Immunohistochemistry-Paraffin: SLC4A7/Sodium bicarbonate cotransporter 3 Antibody [NBP1-87850] - Analysis in human duodenum and liver tissues. Corresponding SLC4A7 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/SLC4A7-Sodium-bicarbonate-cotransporter-3-Antibody-Immunohistochemistry-Paraffin-NBP1-87850-img0007.jpg)
![Immunohistochemistry-Paraffin: SLC4A7/Sodium bicarbonate cotransporter 3 Antibody [NBP1-87850] - Staining of human tonsil shows moderate to strong membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize2/SLC4A7-Sodium-bicarbonate-cotransporter-3-Antibody-Immunohistochemistry-Paraffin-NBP1-87850-img0008.jpg)
![Western Blot: NHE1/SLC9A1 Antibody [NBP1-76847] - Rat kidney tissue lysate with in with Nhe-1 antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize2/NHE1-SLC9A1-Antibody-Western-Blot-NBP1-76847-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: NHE1/SLC9A1 Antibody [NBP1-76847] - Immunofluorescence of Nhe-1 in Human Brain tissue with Nhe-1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize2/NHE1-SLC9A1 Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76847-img0003.jpg)
![Immunohistochemistry-Paraffin: NHE1/SLC9A1 Antibody [NBP1-76847] - Human brain tissue using Nhe-1 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize2/NHE1-SLC9A1-Antibody-Immunohistochemistry-Paraffin-NBP1-76847-img0002.jpg)
![Western Blot: SLC4A5 Antibody [NBP1-59528] - THP-1 cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize2/SLC4A5 Antibody-Western Blot-NBP1-59528-img0002.jpg)
![Immunohistochemistry-Paraffin: SLC4A10 Antibody [NBP1-82594] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells and neuropil.](http://images.novusbio.com/fullsize2/SLC4A10-Antibody-Immunohistochemistry-Paraffin-NBP1-82594-img0001.jpg)
![Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Lane 1: Carbonic Anhydrase II. Lane 2: None. Load: 50 ng per lane. Primary antibody: Carbonic Anhydrase II primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 29 kDa, 29 kDa for Carbonic Anhydrase II. Other band(s): None.](http://images.novusbio.com/fullsize2/Carbonic-Anhydrase-II-CA2-Antibody-Western-Blot-NB600-919-img0007.jpg)
![Immunohistochemistry: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Staining in nucleus and cytoplasm of proximal and distal tubules, bowman's capsule and glomerular podocytes of human kidney (B) and in basal cells of retina of a mouse eye (C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-carbonic anhydrase II antibody at 4.0 ug/ml for 60 minutes. The reaction was developed using either MACH 1 universal HRP polymer detection (human kidney) or MACH 4 universal AP polymer detection system (mouse eye) and visualized with 3'3-diamino-benzidine substrate (DAB) or WARP RED.](http://images.novusbio.com/fullsize2/Carbonic-Anhydrase-II-CA2-Antibody-Immunohistochemistry-NB600-919-img0005.jpg)
![Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Detection of Carbonic Anhydrase II in mouse embryonic whole cell lysate. Image provided via product review by verified customer.](http://images.novusbio.com/fullsize2/Carbonic-Anhydrase-II-CA2-Antibody-Western-Blot-NB600-919-img0001.jpg)
![Western Blot: SLC26A4 Antibody [NBP1-60106] - Titration: 0.2-1 ug/ml, Positive Control: COLO205 cell lysate.](http://images.novusbio.com/fullsize2/SLC26A4 Antibody-Western Blot-NBP1-60106-img0004.jpg)
![Immunohistochemistry-Paraffin: SLC26A4 Antibody [NBP1-60106] - Human placenta tissue at an antibody concentration of 5ug/ml.](http://images.novusbio.com/fullsize2/SLC26A4 Antibody-Immunohistochemistry-Paraffin-NBP1-60106-img0005.jpg)
![Immunohistochemistry-Paraffin: SLC4A9 Antibody [NBP2-13340] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize2/SLC4A9-Antibody-Immunohistochemistry-Paraffin-NBP2-13340-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: AE2 Antibody [NBP1-90225] - Staining of human cell line A-431 shows localization to nuclear speckles, plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/AE2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90225-img0005.jpg)
![Immunohistochemistry-Paraffin: AE2 Antibody [NBP1-90225] - Staining in human placenta and tonsil tissues using anti-SLC4A2 antibody. Corresponding SLC4A2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/AE2-Antibody-Immunohistochemistry-Paraffin-NBP1-90225-img0011.jpg)
![Immunohistochemistry-Paraffin: AE2 Antibody [NBP1-90225] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize2/AE2-Antibody-Immunohistochemistry-Paraffin-NBP1-90225-img0009.jpg)
![Western Blot: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - Analysis of Angiotensinogen in human kidney lysate.](http://images.novusbio.com/fullsize2/Serpin-A8-Angiotensinogen-Antibody-Western-Blot-NBP1-30027-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Angiotensinogen at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/Serpin-A8-Angiotensinogen-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30027-img0014.jpg)
![Immunohistochemistry-Paraffin: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - Analysis of Angiotensinogen in mouse kidney using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize2/Serpin-A8-Angiotensinogen-Antibody-Immunohistochemistry-Paraffin-NBP1-30027-img0012.jpg)
![Western Blot: NHE3/SLC9A3 [p Ser552] Antibody (14D5) [NB110-81529] - Detection of NHE3 [p Ser552] in human kidney lysate.](http://images.novusbio.com/fullsize2/NHE3-SLC9A3-[p-Ser552]-Antibody-(14D5)-Western-Blot-NB110-81529-img0005.jpg)
![Simple Western: NHE3/SLC9A3 [p Ser552] Antibody (14D5) [NB110-81529] - Simple Western lane view shows a specific band for NHE3 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize2/NHE3-SLC9A3-[p-Ser552]-Antibody-(14D5)-Simple-Western-NB110-81529-img0006.jpg)
![Immunofluorescence: NHE3/SLC9A3 [p Ser552] Antibody (14D5) [NB110-81529] - Mouse intestine, image courtesy of verified customer review.](http://images.novusbio.com/fullsize2/NHE3-SLC9A3-[p-Ser552]-Antibody-(14D5)-Immunofluorescence-NB110-81529-img0008.jpg)
![Immunohistochemistry-Paraffin: Glutathione S Transferase kappa 1 Antibody [NBP1-89727] - Staining of human small intestine shows high expression.](http://images.novusbio.com/fullsize2/Glutathione-S-Transferase-kappa-1-Antibody-Immunohistochemistry-Paraffin-NBP1-89727-img0008.jpg)
![Immunohistochemistry-Paraffin: Glutathione S Transferase kappa 1 Antibody [NBP1-89727] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/Glutathione-S-Transferase-kappa-1-Antibody-Immunohistochemistry-Paraffin-NBP1-89727-img0002.jpg)
![Immunohistochemistry-Paraffin: Glutathione S Transferase kappa 1 Antibody [NBP1-89727] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize2/Glutathione-S-Transferase-kappa-1-Antibody-Immunohistochemistry-Paraffin-NBP1-89727-img0005.jpg)
![Immunohistochemistry-Paraffin: SLCO6A1 Antibody [NBP2-49118] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize2/SLCO6A1-Antibody-Immunohistochemistry-Paraffin-NBP2-49118-img0004.jpg)
![Immunohistochemistry-Paraffin: SLCO6A1 Antibody [NBP2-49118] - Staining of human endometrium shows low expression as expected.](http://images.novusbio.com/fullsize2/SLCO6A1-Antibody-Immunohistochemistry-Paraffin-NBP2-49118-img0003.jpg)
![Immunohistochemistry-Paraffin: SLCO6A1 Antibody [NBP2-49118] - Staining in human testis and endometrium tissues using anti-SLCO6A1 antibody. Corresponding SLCO6A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/SLCO6A1 Antibody-Immunohistochemistry-Paraffin-NBP2-49118-img0005.jpg)
![Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Insulin E2E3, human pancreas (FFPE), HIER pH 9 antigen retrieval. Image from verified customer review.](http://images.novusbio.com/fullsize2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Immunohistochemistry-Paraffin-NBP2-34260-img0004.jpg)
![Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)](http://images.novusbio.com/fullsize2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Immunohistochemistry-Paraffin-NBP2-34260-img0002.jpg)
![SDS-Page: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260]](http://images.novusbio.com/fullsize2/Insulin Antibody (E2-E3 (same as INS04))-SDS-Page-NBP2-34260-img0003.jpg)
![Western Blot: Catalase Antibody [NBP2-24916] - Analysis in liver lysate of 1) human 2) mouse and 3) rat at 0.1 ug/ml.](http://images.novusbio.com/fullsize2/Catalase-Antibody-Western-Blot-NBP2-24916-img0001.jpg)
![Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-24916] - Staining of human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize2/Catalase-Antibody-Immunohistochemistry-Paraffin-NBP2-24916-img0002.jpg)
![Immunohistochemistry-Paraffin: SLC12A3 Antibody [NBP1-86376] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize2/SLC12A3-Antibody-Immunohistochemistry-Paraffin-NBP1-86376-img0007.jpg)
![Immunohistochemistry-Paraffin: SLC12A3 Antibody [NBP1-86376] - Staining of human kidney shows high expression.](http://images.novusbio.com/fullsize2/SLC12A3-Antibody-Immunohistochemistry-Paraffin-NBP1-86376-img0006.jpg)
![Immunohistochemistry-Paraffin: SLC12A3 Antibody [NBP1-86376] - Staining in human kidney and lymph node tissues using anti-SLC12A3 antibody. Corresponding SLC12A3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/SLC12A3 Antibody-Immunohistochemistry-Paraffin-NBP1-86376-img0008.jpg)
