Adrenomedullin R/ADMR/GPR182 Antibody (S02-3E6)

Western Blot: Adrenomedullin R/ADMR/GPR182 Antibody (S02-3E6) [NBP3-19650] - Western blot detection of Adrenomedullin R/ADMR/GPR182 in K562, C6, 3T3, Hela cell lysates using NBP3-19650 (1:1000 diluted). Predicted band size: 45kDa. Observed band size: 45kDa.

• Reactivity: Hu, Mu
• Applications: WB
• Clone: S02-3E6
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

Adrenomedullin R/ADMR/GPR182 Antibody (S02-3E6) Summary

• Description: Novus Biologicals Rabbit Adrenomedullin R/ADMR/GPR182 Antibody (S02-3E6) (NBP3-19650) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein of human Adrenomedullin R/ADMR/GPR182 (Uniprot # O15218)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GPR182
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/1000
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for Adrenomedullin R/ADMR/GPR182 Antibody (S02-3E6)

• 7TMR
• ADMR
• Adrenomedullin R
• adrenomedullin receptor
• AdrenomedullinR
• AMR
• AM-R
• G protein-coupled receptor 182,7TMR
• G10D
• GAMRH
• Gpcr22
• GPR182
• G-protein coupled receptor 182
• hrhAMR
• MB10
• MGC34399
• NOW

Background

G Protein-Coupled Receptor L1/G10D has been suggested to be an Adrenomedullin Receptor. Originally cloned from rats and later from humans, L1/G10D does not to bind adrenomedullin or display functional cAMP response to adrenomedullin (reviewed in Poyner et al. 2002). The initial experimental results, which demonstrated high-affinity binding and increased levels of cAMP in COS cells that were transfected with L1/G10D and treated with adrenomedullin, could not be reproduced by other laboratories (Kapas et al. 1995). It is now known that the functional adrenomedullin receptor is a heterodimeric complex composed of calcitonin receptor-like receptor (CRLR) and receptor activity modifying proteins (RAMPs) (McLatchie et al. 1998). L1/G10D therefore should be considered an Orphan A receptor. L1/G10D expression has been reported in adrenal gland, bone marrow, brain, heart, kidney, liver, lung, lymph node, pancreas, skeletal muscle, small intestine, spleen, stomach, testis, and thyroid. ESTs have been isolated from kidney, liver/spleen, fetal lung/testis/B-cell, and melanocyte/uterus/fetal heart libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPR182