Adenosine Deaminase/ADA Antibody

Images

 
Western Blot: Adenosine Deaminase/ADA Antibody [NBP1-77775] - Lane 1: Adenosine Deaminase (Calf Spleen). Lane 2: None. Load: 50 ng per lane. Used at a dilution of 1:1,000 overnight at 4C.
Western Blot: Adenosine Deaminase/ADA Antibody [NBP1-77775] - Used to detect adenosine deaminase in mouse pancreas lysate (Left, Lane 1, 30 ul) under reducing conditions. The antibody was also used to detect purified ...read more

Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications WB, ELISA, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Catalog# & Conjugate Size Price

Adenosine Deaminase/ADA Antibody Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Adenosine Deaminase [Calf Spleen]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ADA
Purity
Ion exchange chromatography
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Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:5000
  • ELISA 1:5000 - 1:20000
  • Immunoprecipitation 1:100
Application Notes
This product has been assayed against 1.0 ug of Adenosine Deaminase [Calf Spleen] by immunoblot using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] and DAB as a substrate at room temperature. A working dilution of 1:8,000 - 1:40,000 is suggested for this product. This product has been assayed against 1.0 ug of Adenosine Deaminase [Calf Spleen] by immunoblot using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] and DAB as a substrate at room temperature. A working dilution of 1:8,000 - 1:40,000 is suggested for this product. This product has been assayed against 1.0 ug of Adenosine Deaminase [Calf Spleen] by immunoblot using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] and DAB as a substrate at room temperature. A working dilution of 1:8,000 - 1:40,000 is suggested for this product.
Control
Adenosine Deaminase/ADA Lysate (NBP2-65443)

Reactivity Notes

Reactive in Bovine Calf spleen

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Ion exchange chromatography

Alternate Names for Adenosine Deaminase/ADA Antibody

  • ADA
  • ADA1
  • adenine deaminase
  • Adenosine aminohydrolase
  • Adenosine Deaminase
  • EC 3.5.4.4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ADA