Adenosine A2aR Antibody

Western Blot: Adenosine A2aR Antibody [NB300-597] - Analysis of 25 ug of human placenta (lane 1), HepG2 (lane 2), Hela (lane 3) and mouse liver (lane 4) cell lysates.

Simple Western: Adenosine A2aR Antibody [NB300-597] - Simple Western lane view shows a specific band for Adenosine A2a R in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunohistochemistry-Paraffin: Adenosine A2aR Antibody [NB300-597] - Analysis showing positive staining in the cytoplasm and membrane of Human placenta tissue (right) compared with a negative control in the absence of primary antibody (left).

Immunocytochemistry/Immunofluorescence: Adenosine A2aR Antibody [NB300-597] - Analysis of Adenosine Receptor A2a (green) showing staining in the cytoplasm of U251 cells (right) compared to a negative control without primary antibody (left). F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI

Immunocytochemistry/Immunofluorescence: Adenosine A2aR Antibody [NB300-597] - Mixed cortical rat neurons culture (21 DIV) stained for A2A labelled with Alexa647. The staining visualised the astrocytes in culture. This image was submitted via customer Review.

Immunohistochemistry-Paraffin: Adenosine A2aR Antibody [NB300-597] - Staining in the cytoplasm and membrane of Mouse testis tissue (right) compared with a negative control in the absence of primary antibody (left).

Immunohistochemistry-Paraffin: Adenosine A2aR Antibody [NB300-597] - Analysis showing positive staining in the cytoplasm and membrane of Human testis tissue (right) compared with a negative control in the absence of primary antibody (left).

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, Simple Western, Flow, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.69 mg/ml

Adenosine A2aR Antibody Summary

• Description: Novus Biologicals Rabbit Adenosine A2aR Antibody (NB300-597) is a polyclonal antibody validated for use in IHC, WB, Flow, ICC/IF, Simple Western and IP. Anti-Adenosine A2aR Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide corresponding to residues E(373) S H G D M G L P D V E L L S H E L K(391) of canine A2aAR.
• Localization: Cell Membrane
• Specificity: Detects adenosine receptor A2a. This does not detect other AR subtypes.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ADORA2A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:20-1:200
  • Immunoprecipitation 1:10-1:500
  • Simple Western 1:20
  • Western Blot 1:1000
• Application Notes: Use in Flow reported in scientific literature (PMID:34536555). WB: Detects an approx. 45 kDa protein from canine striatum representing A2aAR.
  
  See Simple Western Antibody Database for Simple Western validation: tested in HeLa lysate (0.5 mg/ml); antibody dilution of 1:20; separated by size; detects a band at 51 kDa
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.69 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Adenosine A2aR Antibody

• A2aR
• adenosine A2 receptor
• Adenosine A2a R
• adenosine A2a receptor
• Adenosine A2aR
• adenosine receptor A2a
• adenosine receptor subtype A2a
• ADORA 2
• ADORA2
• ADORA2A
• hA2aR
• RDC8

Background

Adenosine receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily. Structural, biochemical and pharmacological analyses of the AR genes and protein has led to the discovery of four distinct AR subtypes (A1, A2a, A2b, A3). Activation of ARs mediates several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission. The A2aAR is a glycoprotein that can activate adenylyl cyclase via Gs interactions. Stimulation of A2aAR and the resultant accumulation of cAMP inhibits platelet aggregation and in certain vascular beds is associated with vasodilation and drop in blood pressure. A2aAR is reported to interact with D2 dopamine receptor sites in the brain where they co-express (striatum, olfactory tubercle and nucleus accumbens). Other tissues including heart, kidney, and lung also express A2aAR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Adenosine A2aR Antibody (NB300-597)(3)

Publications using NB300-597

• Du H, Li CH, Gao RB et al. Inhibition of the interaction between microglial adenosine 2A receptor and NLRP3 inflammasome attenuates neuroinflammation posttraumatic brain injury CNS neuroscience & therapeutics 2023-08-11 [PMID: 37564004]
• Pang L, Ng KT, Liu J et al. Plasmacytoid dendritic cells recruited by HIF-1 alpha /eADO/ADORA1 signaling induce immunosuppression in hepatocellular carcinoma Cancer letters 2021-09-16 [PMID: 34536555] (FLOW, Human)
• Ren ZH, Lin CZ, Cao W et al. CD73 is associated with poor prognosis in HNSCC. Oncotarget. 2016-09-20 [PMID: 27557512] (IF/IHC, WB, Human)

Review for Adenosine A2aR Antibody (NB300-597) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Janosch Heller ICC Rat 06/27/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Primary rat cortical neurons
• Species: Rat

Comments

• Comments: Fixation Solution and Conditions: 4% paraformaldehyde in PBS, 15 minutes RT
  
  Blocking Solution & Duration: 3% BSA in PBS with 0.2% saponin, 30 minutes RT
  
  Primary Antibody Diluent and Dilutions Tested: 1:120 in 1% BSA in PBS with 0.2% saponin, over night 4°C
  
  Secondary Antibody Manufacturer, Host Species, Dilution, & Diluent: Thermo donkey anti-rabbit Alexa647, 1:500 in PBS with 0.2% saponin, 1 hour, RT

Bioinformatics

• Gene Symbol: ADORA2A
• Entrez:
  • 135(Human)
  • 25369(Rat)