Adenosine A2aR Antibody

Images

 
Western Blot: Adenosine A2a R Antibody [NB300-597] - Analysis of 25 ug of human placenta (lane 1), HepG2 (lane 2), Hela (lane 3) and mouse liver (lane 4) cell lysates.
Simple Western: Adenosine A2a R Antibody [NB300-597] - Simple Western lane view shows a specific band for Adenosine A2a R in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the ...read more
Immunohistochemistry-Paraffin: Adenosine A2a R Antibody [NB300-597] - Analysis showing positive staining in the cytoplasm and membrane of Human placenta tissue (right) compared with a negative control in the absence of ...read more
Immunocytochemistry/ Immunofluorescence: Adenosine A2a R Antibody [NB300-597] - Analysis of Adenosine Receptor A2a (green) showing staining in the cytoplasm of U251 cells (right) compared to a negative control without ...read more
Immunocytochemistry: Adenosine A2a R Antibody [NB300-597] - Mixed cortical rat neurons culture (21 DIV) stained for A2A labelled with Alexa647. The staining visualised the astrocytes in culture. This image was submitted ...read more
Immunohistochemistry-Paraffin: Adenosine A2a R Antibody [NB300-597] - Staining in the cytoplasm and membrane of Mouse testis tissue (right) compared with a negative control in the absence of primary antibody (left).
Immunohistochemistry-Paraffin: Adenosine A2a R Antibody [NB300-597] - Analysis showing positive staining in the cytoplasm and membrane of Human testis tissue (right) compared with a negative control in the absence of ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, IP, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.69 mg/ml

Order Details

Adenosine A2aR Antibody Summary

Immunogen
Synthetic peptide corresponding to residues E(373) S H G D M G L P D V E L L S H E L K(391) of canine A2aAR.
Localization
Cell Membrane
Specificity
Detects adenosine receptor A2a. This does not detect other AR subtypes.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ADORA2A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:20
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:20-1:200
  • Immunoprecipitation 1:10-1:500
  • Immunocytochemistry
Application Notes
WB: Detects an approx. 45 kDa protein from canine striatum representing A2aAR.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB300-597 in the following applications:

Publications
Read Publication using
NB300-597 in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
0.69 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Adenosine A2aR Antibody

  • A2aR
  • adenosine A2 receptor
  • Adenosine A2a R
  • adenosine A2a receptor
  • Adenosine A2aR
  • adenosine receptor A2a
  • adenosine receptor subtype A2a
  • ADORA 2
  • ADORA2
  • ADORA2A
  • hA2aR
  • RDC8

Background

Adenosine receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily. Structural, biochemical and pharmacological analyses of the AR genes and protein has led to the discovery of four distinct AR subtypes (A1, A2a, A2b, A3). Activation of ARs mediates several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission. The A2aAR is a glycoprotein that can activate adenylyl cyclase via Gs interactions. Stimulation of A2aAR and the resultant accumulation of cAMP inhibits platelet aggregation and in certain vascular beds is associated with vasodilation and drop in blood pressure. A2aAR is reported to interact with D2 dopamine receptor sites in the brain where they co-express (striatum, olfactory tubercle and nucleus accumbens). Other tissues including heart, kidney, and lung also express A2aAR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Adenosine A2aR Antibody (NB300-597)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC, WB.


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IHC
(1)
WB
(1)
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Showing Publication 1 - 1 of 1.
Publication using NB300-597 Applications Species
Ren ZH, Lin CZ, Cao W et al. CD73 is associated with poor prognosis in HNSCC. Oncotarget. Sep 20 2016 [PMID: 27557512] (WB, IHC, Human) WB, IHC Human

Review for Adenosine A2aR Antibody (NB300-597) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB300-597:
Filter by Applications
ICC
(1)
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Filter by Species
Rat
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry Adenosine A2aR NB300-597
Enlarge
reviewed by:
Janosch Heller
ICC Rat 06/27/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedPrimary rat cortical neurons
SpeciesRat

Comments

CommentsFixation Solution and Conditions: 4% paraformaldehyde in PBS, 15 minutes RT

Blocking Solution & Duration: 3% BSA in PBS with 0.2% saponin, 30 minutes RT

Primary Antibody Diluent and Dilutions Tested: 1:120 in 1% BSA in PBS with 0.2% saponin, over night 4°C

Secondary Antibody Manufacturer, Host Species, Dilution, & Diluent: Thermo donkey anti-rabbit Alexa647, 1:500 in PBS with 0.2% saponin, 1 hour, RT

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Adenosine A2aR Antibody (NB300-597) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Janosch Heller
06/27/2017
Application: ICC
Species: Rat

Bioinformatics

Gene Symbol ADORA2A
Entrez