ADAMTS5 Antibody

Western Blot: ADAMTS5 Antibody [NBP2-15286] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAMTS5 antibody [N3C2], Internal diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: ADAMTS5 Antibody [NBP2-15286] - Human hepatoma. ADAMTS5 antibody [N3C2], Internal dilution: 1:250. Scale bar = 100 um. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: ADAMTS5 Antibody [NBP2-15286] - Sample (30 ug of whole cell lysate) A: HeLa B: HepG2 7. 5% SDS PAGE, antibody diluted at 1:3000.

Immunohistochemistry-Paraffin: ADAMTS5 Antibody [NBP2-15286] - Human hepatoma, using ADAMTS5 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Rt, Mu, Bv
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

ADAMTS5 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human ADAMTS5. The exact sequence is proprietary.
• Predicted Species: Mouse (98%), Bovine (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ADAMTS5
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000
• Theoretical MW: 102 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (84%). .

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for ADAMTS5 Antibody

• A disintegrin and metalloproteinase with thrombospondin motifs 11
• a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 5 (aggrecanase-2)
• ADAM metallopeptidase with thrombospondin type 1 motif, 5
• ADAM-TS 11
• ADAM-TS 5
• ADAMTS11
• ADAMTS-11
• ADAMTS5
• ADAM-TS5
• ADAMTS-5
• ADMP2
• ADMP-2
• ADMP-2A disintegrin and metalloproteinase with thrombospondin motifs 5
• Aggrecanase 2
• aggrecanase-2
• disintegrin-like and metalloprotease with thrombospondin type 1 motif, 510ADAMTS11FLJ36738
• EC 3.4.24
• EC 3.4.24.-
• EC 3.4.24.14
• EC 3.4.24.82

Background

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene contains two C-terminal TS motifs and functions as aggrecanase to cleave aggrecan, a major proteoglycan of cartilage. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for ADAMTS5 Antibody (NBP2-15286)(6)

Publications using NBP2-15286

• Yagi M, Endo K, Komori K, Sekiya I Comparison of the effects of oxidative and inflammatory stresses on rat chondrocyte senescence Scientific reports 2023-05-11 [PMID: 37169906] (WB, Rat)
  
  Details:
  Dilution: 1:1000
• Li P, Lv S, Jiang W et al. Exosomes derived from umbilical cord mesenchymal stem cells protect cartilage and regulate the polarization of macrophages in osteoarthritis Annals of Translational Medicine 2022-09-01 [PMID: 36267713] (Western Blot, Human)
• Bai H, Yuan R, Zhang Z et al. Intra-articular Injection of Baicalein Inhibits Cartilage Catabolism and NLRP3 Inflammasome Signaling in a Posttraumatic OA Model Oxidative medicine and cellular longevity 2021-09-02 [PMID: 34512868] (IF/IHC, Rat)
• Huang Y, Huang L, Li L et al. MicroRNA-25-3p therapy for intervertebral disc degeneration by targeting the IL-1?/ZIP8/MTF1 signaling pathway with a novel thermo-responsive vector Annals of Translational Medicine 2020-12-14 [PMID: 33313245] (Western Blot, Human)
• Si HB, Yang TM, Li L et al. miR-140 Attenuates the Progression of Early-Stage Osteoarthritis by Retarding Chondrocyte Senescence Mol Ther Nucleic Acids 2019-11-09 [PMID: 31790972] (WB, IHC-P, Human, Rat)
• Ding Lei, Zampogna Biagio, Vasta Sebastiano et al. Why Do Osteochondral Allografts Survive. CompaRative Analysis of Cartilage Biochemical Properties Unveils a Molecular Basis for Durability. The American Journal of Sports Medicine 2015-01-01 [PMID: 26311444] (WB, Human)

Bioinformatics

• Gene Symbol: ADAMTS5
• Uniprot:
  • Q9UNA0()