ADAMTS5 Antibody

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Product Details

Summary
Reactivity Hu, Po, BvSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ADAMTS5 Antibody Summary

Immunogen
Synthetic peptide corresponding to residues C K(636) N G Y Q S D A K G V K T F(649) of human ADAMTS5.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ADAMTS5
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
Application Notes
WB: Detects an major band at approx. 68 kDa protein representing ADAMTS5 from porcine cartilage extract. On human recombinant ADAMTS5 WB: Detects a major band at approx. 85 kDa. Depending on the extent of C-terminal processing, several smaller bands may also be seen using this antibody.
Positive Control
ADAMTS5 Lysate (NBL1-07317)

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS containing 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ADAMTS5 Antibody

  • A disintegrin and metalloproteinase with thrombospondin motifs 11
  • a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 5 (aggrecanase-2)
  • ADAM metallopeptidase with thrombospondin type 1 motif, 5
  • ADAM-TS 11
  • ADAM-TS 5
  • ADAMTS11
  • ADAMTS-11
  • ADAMTS5
  • ADAM-TS5
  • ADAMTS-5
  • ADMP2
  • ADMP-2
  • ADMP-2A disintegrin and metalloproteinase with thrombospondin motifs 5
  • Aggrecanase 2
  • aggrecanase-2
  • disintegrin-like and metalloprotease with thrombospondin type 1 motif, 510ADAMTS11FLJ36738
  • EC 3.4.24
  • EC 3.4.24.-
  • EC 3.4.24.14
  • EC 3.4.24.82

Background

ADAMTS5, also known as Implantin or Aggrecanase 2, is a member of the larger family of ADAM (A Disintegrin And Metalloproteinase) metalloproteinases containing thrombospondin (TS) repeats. ADAMTS5 (A Disintegrin And Metalloproteinase with Thrombospondin-5 motif) was first described as ADAMTS5, a protein elevated in mice during the peri-implantion period. At the same time, another group identified Aggrecanase 11, a protein elevated in arthritic synovium. The name was later changed to ADAMTS5. ADAMTS5 is expressed in human and mouse. It has been found in heart, lung, cervix, uterus, ovary, brain, cartilage, and numerous other tissues, as well as chondroblastoma cell lines. Initial observations indicated a role for ADAMTS5 in aggrecan cleavage and cartilage destruction, especially in arthritis, and potentially a role in embryo implantation. ADAMTS5 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to efficiently cleave Aggrecan. In addition to the metalloprotease domain, ADAMTS5 has a propeptide domain, a prohormone convertase (PC, furin) cleavage site, a cysteine-rich domain, a spacer domain, and two thrombospondin-1 like domains. ADAMTS5 is inhibited by the endogenous MMP inhibitors (TIMP1, 2, 3, and 4) but most efficiently by TIMP3. Unlike many of the ADAMs proteases, ADAMTS5 does not have a transmembrane domain, and is a secreted protein. Full length ADAMTS5 is a 930 amino acid protein with a predicted molecular mass is 101.7 kDa, but glycosylation and the abundance of cysteine residues gives ADAMTS5 a greater apparent molecular weight on reduced SDS PAGE gels. When ADAMTS5 is secreted, it is cleaved at the furin cleavage site (predicted molecular mass 73.2 kDa) and then further cleaved to generate a range of smaller forms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ADAMTS5
Entrez
Uniprot