ADAMTS5 Antibody (PSH03-52)

Immunocytochemistry/ Immunofluorescence: ADAMTS5 Antibody (PSH03-52) [NBP3-32001] - Immunocytochemistry analysis of HepG2 cells labeling ADAMTS5 with Rabbit anti-ADAMTS5 antibody (NBP3-32001) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ADAMTS5 antibody (NBP3-32001) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

Flow Cytometry: ADAMTS5 Antibody (PSH03-52) [NBP3-32001] - Flow cytometric analysis of HepG2 cells labeling ADAMTS5. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32001, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Western Blot: ADAMTS5 Antibody (PSH03-52) [NBP3-32001] - Western blot analysis of ADAMTS5 on different lysates with Rabbit anti-ADAMTS5 antibody (NBP3-32001) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: LO2 cell lysate Lane 3: Huh7 cell lysate Lane 4: HeLa cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: Human liver tissue lysate Lane 8: Mouse placenta tissue lysate Lane 9: Mouse uterus tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 102 kDa Observed band size: 75 kDa Exposure time: 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32001) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF
• Clone: PSH03-52
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

ADAMTS5 Antibody (PSH03-52) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within human ADAMTS5 aa 251-500 / 930. (Uniprot: Q9UNA0)
• Localization: Secreted, extracellular space, extracellular matrix.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ADAMTS5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:250
  • Western Blot 1:2000
• Theoretical MW: 102 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ADAMTS5 Antibody (PSH03-52)

• A disintegrin and metalloproteinase with thrombospondin motifs 11
• a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 5 (aggrecanase-2)
• ADAM metallopeptidase with thrombospondin type 1 motif, 5
• ADAM-TS 11
• ADAM-TS 5
• ADAMTS11
• ADAMTS-11
• ADAMTS5
• ADAM-TS5
• ADAMTS-5
• ADMP2
• ADMP-2
• ADMP-2A disintegrin and metalloproteinase with thrombospondin motifs 5
• Aggrecanase 2
• aggrecanase-2
• disintegrin-like and metalloprotease with thrombospondin type 1 motif, 510ADAMTS11FLJ36738
• EC 3.4.24
• EC 3.4.24.-
• EC 3.4.24.14
• EC 3.4.24.82

Background

ADAMTS5, also known as aggrecanase-2, is a Metallopeptidase M12B type protease that is responsible for degradation of the articular cartilage protein aggrecan. The protein contains a signal sequence, a prodomain with both a potential cysteine switch and a furin cleavage site, a metalloproteinase domain with a conserved zinc-binding motif and a 'met turn,' a disintegrin-like domain, and a spacer region between a thrombospondin type 1 motif and thrombospondin submotif. At least four transcript variants have been reported. ADAMTS5 has been implicated in inflammatory joint diseases. ADAMTS5 expression has been documented in a range of normal human tissues. ESTs have been isolated from human tissue libraries, including normal breast, embryo and heart and cancerous placenta and uterus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ADAMTS5