ADAMTS4 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

ADAMTS4 Antibody [Biotin] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human ADAMTS4
  Phe213-Cys685
  Accession # O75173
• Specificity: Detects human ADAMTS4 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ADAMTS4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADAMTS4 Antibody [Biotin]

• a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 4
• ADAM metallopeptidase with thrombospondin type 1 motif, 4
• ADAM-TS 4
• ADAMTS-2
• ADAMTS4
• ADAM-TS4
• ADAMTS-4
• ADMP-1
• ADMP-1EC 3.4.24.82
• Aggrecanase 1
• aggrecanase-1
• EC 3.4.24
• KIAA0688A disintegrin and metalloproteinase with thrombospondin motifs 4

Background

ADAMTS4 (a disintegrin and metalloproteinase with thrombospondin motifs 4), also known as aggrecanase-1, is a member of the family of secreted zinc proteases with a multi-domain structure (1‑3). The protein precursors consist of a signal peptide and the following domains: pro, catalytic, disintegrin-like, TS type 1 motif, cysteine-rich, and spacer. It is the only ADAMTS identified that has one TS type I motif. It is an active protease effectively cleaving alpha -2-macroglobulin and aggrecan at multiple sites, and is inhibited by TIMP-3 with inhibition constants in subnanomolar range (4‑6). It receives great attention due to the elevation in its mRNA level after treatment with Interleukin-1 (7). However, in a mouse model of osteoarthritis, ADAMTS4 knock-out mice did not exhibit any significant protective effect (8). The purified rhADAMTS4 starts at the catalytic domain and ends before the spacer domain. If desired, the aggrecanase activity can be inhibited by 5 mM 1,10‑phenanthroline and rhTIMP-3 (R&D Systems, Catalog # 973-TM).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ADAMTS4 Antibody (BAF4307)(1)

Publication using BAF4307

• Shane D. Wainwright, Jan Bondeson, Bruce Caterson, Clare E. Hughes ADAMTS‐4_v1 Is a Splice Variant of ADAMTS‐4 That Is Expressed as a Protein in Human Synovium and Cleaves Aggrecan at the Interglobular Domain Arthritis & Rheumatism 2013-11-01 [PMID: 23897278]

Bioinformatics

• Gene Symbol: ADAMTS4
• Uniprot:
  • O75173()