ADAMTS4 Antibody [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

ADAMTS4 Antibody [Alexa Fluor® 488] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human ADAMTS4
  Phe213-Cys685
  Accession # O75173
• Specificity: Detects human ADAMTS4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) ADAMTS5 is observed and less than 1% cross-reactivity with rhADAMTS13, rhADAMTS-L1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADAMTS4 Antibody [Alexa Fluor® 488]

• a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 4
• ADAM metallopeptidase with thrombospondin type 1 motif, 4
• ADAM-TS 4
• ADAMTS-2
• ADAMTS4
• ADAM-TS4
• ADAMTS-4
• ADMP-1
• ADMP-1EC 3.4.24.82
• Aggrecanase 1
• aggrecanase-1
• EC 3.4.24
• KIAA0688A disintegrin and metalloproteinase with thrombospondin motifs 4

Background

ADAMTS4 (a disintegrin and metalloproteinase with thrombospondin motifs 4), also known as aggrecanase-1, is a member of the family of secreted zinc proteases with a multi-domain structure (1‑3). The protein precursors consist of a signal peptide and the following domains: pro, catalytic, disintegrin-like, TS type 1 motif, cysteine-rich, and spacer. It is the only ADAMTS identified that has one TS type I motif. It is an active protease effectively cleaving alpha -2-macroglobulin and aggrecan at multiple sites, and is inhibited by TIMP-3 with inhibition constants in subnanomolar range (4‑6). It receives great attention due to the elevation in its mRNA level after treatment with Interleukin-1 (7). However, in a mouse model of osteoarthritis, ADAMTS4 knock-out mice did not exhibit any significant protective effect (8). The purified rhADAMTS4 starts at the catalytic domain and ends before the spacer domain. If desired, the aggrecanase activity can be inhibited by 5 mM 1,10‑phenanthroline and recombinant human TIMP-3 (R&D Systems, Catalog # 973-TM).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.