ADAMTS13 Antibody [Unconjugated]

6156-AD) was serially diluted and captured by Mouse Anti-Human ADAMTS13 Monoclonal Antibody (Catalog # MAB42451) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human ADAMTS13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4245) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" > 6156-AD) was serially diluted and captured by Mouse Anti-Human ADAMTS13 Monoclonal Antibody (Catalog # MAB42451) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human ADAMTS13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4245) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" title="Recombinant Human ADAMTS13 Full Length (Catalog # 6156-AD) was serially diluted and captured by Mouse Anti-Human ADAMTS13 Monoclonal Antibody (Catalog # MAB42451) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human ADAMTS13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4245) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" />

Recombinant Human ADAMTS13 Full Length (Catalog # <a class=

6156-AD) was serially diluted and captured by Mouse Anti-Human ADAMTS13 Monoclonal Antibody (Catalog # MAB42451) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human ADAMTS13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4245) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" > 6156-AD) was serially diluted and captured by Mouse Anti-Human ADAMTS13 Monoclonal Antibody (Catalog # MAB42451) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human ADAMTS13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4245) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" title="Recombinant Human ADAMTS13 Full Length (Catalog # 6156-AD) was serially diluted and captured by Mouse Anti-Human ADAMTS13 Monoclonal Antibody (Catalog # MAB42451) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human ADAMTS13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4245) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" />

Recombinant Human ADAMTS13 Full Length (Catalog # <a class=

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

ADAMTS13 Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human ADAMTS13
  Gln34-Trp688
  Accession # Q76LX8
• Specificity: Detects human ADAMTS13 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) ADAMTS14 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ADAMTS13
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADAMTS13 Antibody [Unconjugated]

• A disintegrin and metalloproteinase with thrombospondin motifs 13
• a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 13
• ADAM metallopeptidase with thrombospondin type 1 motif, 13
• ADAMTS13
• ADAM-TS13
• ADAMTS-13
• DKFZp434C2322
• EC 3.4.24.14
• EC 3.4.24.82
• EC 3.4.24.87
• FLJ42993
• MGC118899
• MGC118900
• TTP
• TTPADAM-TS 13
• vWF-cleaving protease
• vWF-CP
• vWF-CPC9orf8
• VWFCPvon Willebrand factor-cleaving protease

Background

ADAMTS13 (A disintegrin and metalloprotease with TSP motifs 13) is a 140-230 kDa, secreted glycoprotein member of the ADAMTS family of proteases. ADAMTS13 is produced by the liver and circulates in plasma where it cleaves and reduces the activity of von Willebrand factor. ProADAMTS13 is 1398 amino acids (aa) in length. It contains a 45 aa prodomain (aa 30-74), one peptidase domain (aa 80-286) and one disintegrin (aa 287-383) domain, multiple TSP regions and two CUB domains. The prodomain does not affect activity, while the TSP and CUB domains appear to be necessary for activity. One truncated form exists that shows truncation after Gln448. Over aa 34-688, human ADAMST13 shares 76% aa identity with mouse ADAMST13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ADAMTS13
• Uniprot:
  • Q76LX8()