ADAM19 Antibody

Images

 
Western Blot: ADAM19 Antibody [NBP1-69364] - This Anti-ADAM19 antibody was used in Western Blot of Fetal Muscle tissue lysate at a concentration of 1ug/ml.
Western Blot: ADAM19 Antibody [NBP1-69364] - Analysis of ADAM19 in HeLa whole cell lyaste (30ug) using anti-ADAM19 antibody. Image from verified customer review.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ADAM19 Antibody Summary

Immunogen
Synthetic peptides corresponding to ADAM19(ADAM metallopeptidase domain 19 (meltrin beta)) The peptide sequence was selected from the N terminal of ADAM19 (NP_075525). Peptide sequence VADYLEFQKNRRDQDATKHKLIEIANYVDKFYRSLNIRIALVGLEVWTHG.
Marker
Dendritic Cell Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ADAM19
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2-1 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
Application Notes
This is a rabbit polyclonal antibody against ADAM19 and was validated on Western blot. Immunocytochemistry/Immunofluorescence and Immunohistochemistry were reported in scientific literature.
Theoretical MW
82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-69364 in the following applications:

Publications
Read Publications using
NBP1-69364 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for ADAM19 Antibody

  • a disintegrin and metalloproteinase domain 19 (meltrin beta)
  • ADAM 19
  • ADAM metallopeptidase domain 19
  • ADAM19
  • disintegrin and metalloproteinase domain-containing protein 19
  • EC 3.4.24
  • EC 3.4.24.-
  • MADDAM
  • Meltrin beta
  • meltrin-beta
  • Metalloprotease and disintegrin dendritic antigen marker
  • MLTNBmeltrin beta

Background

ADAM19 is a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This member is a type I transmembrane protein and serves as a marker for dendritic cell differentiation. It has also been demonstrated to be an active metalloproteinase, which may be involved in normal physiological and pathological processes such as cells migration, cell adhesion, cell-cell and cell-matrix interactions, and signal transduction. Alternative splicing results in two transcript variants.This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This member is a type I transmembrane protein and serves as a marker for dendritic cell differentiation. It has also been demonstrated to be an active metalloproteinase, which may be involved in normal physiological and pathological processes such as cells migration, cell adhesion, cell-cell and cell-matrix interactions, and signal transduction. Alternative splicing results in two transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ADAM19 Antibody (NBP1-69364)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, IHC.


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ICC/IF
(2)
IHC
(1)
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Mouse
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Review for ADAM19 Antibody (NBP1-69364) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-69364:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot ADAM19 NBP1-69364
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reviewed by:
Anonymous
WB Human 10/06/2014
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Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate from HeLa cells
SpeciesHuman

Blocking

Blocking Details5% NFDM in PBS+0.1% Triton

Primary Anitbody

Dilution Ratio1000 times diluted in PBS, incubated for 2 hours at room temperature

Secondary Antibody

Secondary DescriptionAnti-rabbit IgG, HRP-linked Antibody (Cell Signaling)
Secondary Manufacturer Cat##7074
Secondary Concentration20,000

Details

Detection NotesCaptured using X-rays (30 sec exposure) with Phototope-HRP Western Blot Detection System

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ADAM19 Antibody (NBP1-69364) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for ADAM19 Antibody (NBP1-69364)

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Research Areas for ADAM19 Antibody (NBP1-69364)

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Blogs on ADAM19

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
10/06/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol ADAM19
Entrez
Uniprot