| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB44161-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82791-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33940-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4416-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33939-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89702-50ul | |||
![Immunocytochemistry/Immunofluorescence: ADAM12 Antibody [NB300-889] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic/plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).](http://images.novusbio.com/fullsize/ADAM12-Antibody-Immunocytochemistry-Immunofluorescence-NB300-889-img0005.jpg "Immunocytochemistry/Immunofluorescence: ADAM12 Antibody [NB300-889] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic/plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).")
![Immunohistochemistry-Paraffin: ADAM12 Antibody [NB300-889] - Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/ADAM12-Antibody-Immunohistochemistry-Paraffin-NB300-889-img0003.jpg)
![Flow Cytometry: ADAM12 Antibody [NB300-889] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/ADAM12-Antibody-Flow-Cytometry-NB300-889-img0004.jpg)
![Immunohistochemistry-Paraffin: ADAM12 Antibody [NB300-889] - Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/ADAM12-Antibody-Immunohistochemistry-Paraffin-NB300-889-img0002.jpg)
![ADAM12 expression is upregulated in claudin-low breast cancer cells and in subpopulations enriched for CSCs. a. Cell surface expression of ADAM12 protein in breast cancer cell lines was evaluated by flow cytometry. Red, anti-ADAM12 antibody staining; gray, isotype control antibody staining. b Total cellular expression of ADAM12 was analyzed by Western blotting after partial purification of ADAM12 on concanavalin A (conA) agarose, as described [42]. GAPDH in the input fractions shows comparable conA agarose loading for all cells. Arrow, the nascent full length ADAM12; arrowhead, the processed form lacking the N-terminal pro-domain. c CSC signature score versus ADAM12 mRNA expression in 51 breast cancer cell lines. The CSC signature scores were calculated based on ref. [22] and microarray expression data retrieved from GEO:GSE69017, as described in Methods. Cell lines analyzed in panels a and b are shown in red. d ADAM12 protein levels in total lysates of SUM159PT cells grown as attached monolayers or as mammospheres, analyzed by Western blotting. Arrow, the nascent full length ADAM12; arrowhead, the processed form lacking the N-terminal pro-domain; *, a non-specific band. Positive control represents ADAM12 after partial purification on conA agarose. e Cell surface expression of ADAM12 in SUM159PT cells treated for 6Â days with DMSO (control) or with 10 nM paclitaxel (PTX), and then allowed to recover for 6Â days without PTX, was examined by flow cytometry. The population of cells with the highest expression of ADAM12 is shown in red. FSC, forward scatter Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28148288), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/mab44161_human-adam12-mab-clone-632525-41202412512615.jpg "ADAM12 expression is upregulated in claudin-low breast cancer cells and in subpopulations enriched for CSCs. a. Cell surface expression of ADAM12 protein in breast cancer cell lines was evaluated by flow cytometry. Red, anti-ADAM12 antibody staining; gray, isotype control antibody staining. b Total cellular expression of ADAM12 was analyzed by Western blotting after partial purification of ADAM12 on concanavalin A (conA) agarose, as described [42]. GAPDH in the input fractions shows comparable conA agarose loading for all cells. Arrow, the nascent full length ADAM12; arrowhead, the processed form lacking the N-terminal pro-domain. c CSC signature score versus ADAM12 mRNA expression in 51 breast cancer cell lines. The CSC signature scores were calculated based on ref. [22] and microarray expression data retrieved from GEO:GSE69017, as described in Methods. Cell lines analyzed in panels a and b are shown in red. d ADAM12 protein levels in total lysates of SUM159PT cells grown as attached monolayers or as mammospheres, analyzed by Western blotting. Arrow, the nascent full length ADAM12; arrowhead, the processed form lacking the N-terminal pro-domain; *, a non-specific band. Positive control represents ADAM12 after partial purification on conA agarose. e Cell surface expression of ADAM12 in SUM159PT cells treated for 6Â days with DMSO (control) or with 10 nM paclitaxel (PTX), and then allowed to recover for 6Â days without PTX, was examined by flow cytometry. The population of cells with the highest expression of ADAM12 is shown in red. FSC, forward scatter Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28148288), licensed under a CC-BY license. Not internally tested by R&D Systems.")
![ADAM12 expression is upregulated in claudin-low breast cancer cells and in subpopulations enriched for CSCs. a. Cell surface expression of ADAM12 protein in breast cancer cell lines was evaluated by flow cytometry. Red, anti-ADAM12 antibody staining; gray, isotype control antibody staining. b Total cellular expression of ADAM12 was analyzed by Western blotting after partial purification of ADAM12 on concanavalin A (conA) agarose, as described [42]. GAPDH in the input fractions shows comparable conA agarose loading for all cells. Arrow, the nascent full length ADAM12; arrowhead, the processed form lacking the N-terminal pro-domain. c CSC signature score versus ADAM12 mRNA expression in 51 breast cancer cell lines. The CSC signature scores were calculated based on ref. [22] and microarray expression data retrieved from GEO:GSE69017, as described in Methods. Cell lines analyzed in panels a and b are shown in red. d ADAM12 protein levels in total lysates of SUM159PT cells grown as attached monolayers or as mammospheres, analyzed by Western blotting. Arrow, the nascent full length ADAM12; arrowhead, the processed form lacking the N-terminal pro-domain; *, a non-specific band. Positive control represents ADAM12 after partial purification on conA agarose. e Cell surface expression of ADAM12 in SUM159PT cells treated for 6Â days with DMSO (control) or with 10 nM paclitaxel (PTX), and then allowed to recover for 6Â days without PTX, was examined by flow cytometry. The population of cells with the highest expression of ADAM12 is shown in red. FSC, forward scatter Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28148288), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/mab44161_human-adam12-mab-clone-632525-41202412511347.jpg)
![N/A ADAM12 [HRP]](http://images.novusbio.com/fullsize2/DATA_ADAM12_DAD120_ELISA_564.jpg "N/A ADAM12 [HRP]")
![N/A ADAM12 [HRP]](http://images.novusbio.com/fullsize2/ADAM12_DAD120_ELISA_23.jpg)
![N/A ADAM12 [Biotin]](http://images.novusbio.com/fullsize2/DATA_ADAM12_DY441605_ELISA_2457.jpg "N/A ADAM12 [Biotin]")
![Immunohistochemistry-Paraffin: ADAM12 Antibody [NBP1-82791] - Staining in human placenta and pancreas tissues . Corresponding ADAM12 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ADAM12-Antibody-Immunohistochemistry-Paraffin-NBP1-82791-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ADAM12 Antibody [NBP1-82791] - Staining in human placenta and pancreas tissues . Corresponding ADAM12 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ADAM12 Antibody [NBP1-82791] - Staining of human cell line A-431 shows localization to plasma membrane.](http://images.novusbio.com/fullsize/ADAM12-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82791-img0004.jpg)
![Immunohistochemistry-Paraffin: ADAM12 Antibody [NBP1-82791] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/ADAM12-Antibody-Immunohistochemistry-Paraffin-NBP1-82791-img0008.jpg)
![Immunohistochemistry-Paraffin: ADAM12 Antibody [NBP1-82791] - Staining of human placenta shows moderate positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/ADAM12-Antibody-Immunohistochemistry-Paraffin-NBP1-82791-img0009.jpg)
![Immunohistochemistry-Paraffin: ADAM12 Antibody [NBP2-33940] - Staining in human placenta and liver tissues . Corresponding ADAM12 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ADAM12-Antibody-Immunohistochemistry-Paraffin-NBP2-33940-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ADAM12 Antibody [NBP2-33940] - Staining in human placenta and liver tissues . Corresponding ADAM12 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: ADAM12 Antibody [NBP2-33940] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/ADAM12-Antibody-Western-Blot-NBP2-33940-img0005.jpg)
![Immunohistochemistry-Paraffin: ADAM12 Antibody [NBP2-33940] - Staining of human placenta shows moderate positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/ADAM12-Antibody-Immunohistochemistry-Paraffin-NBP2-33940-img0003.jpg)
![Immunohistochemistry-Paraffin: ADAM12 Antibody [NBP2-33940] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/ADAM12-Antibody-Immunohistochemistry-Paraffin-NBP2-33940-img0004.jpg)
![Immunohistochemistry-Paraffin: ADAM12 Antibody [NBP2-33939] - Staining of human smooth muscles shows moderate positivity.](http://images.novusbio.com/fullsize/ADAM12-Antibody-Immunohistochemistry-Paraffin-NBP2-33939-img0010.jpg "Immunohistochemistry-Paraffin: ADAM12 Antibody [NBP2-33939] - Staining of human smooth muscles shows moderate positivity.")
![Immunohistochemistry-Paraffin: ADAM12 Antibody [NBP2-33939] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/ADAM12-Antibody-Immunohistochemistry-Paraffin-NBP2-33939-img0006.jpg)
![Immunohistochemistry-Paraffin: ADAM12 Antibody [NBP2-33939] - Staining of human placenta shows moderate to strong positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/ADAM12-Antibody-Immunohistochemistry-Paraffin-NBP2-33939-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ADAM12 Antibody [NBP2-33939] - Staining in human placenta and pancreas tissues . Corresponding ADAM12 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ADAM12-Antibody-Immunohistochemistry-Paraffin-NBP2-33939-img0009.jpg)
![Western Blot: ADAM12 Antibody (1G3) [H00008038-M01] - Analysis of ADAM12 expression in transfected 293T cell line by ADAM12 monoclonal antibody (M01), clone 1G3.Lane 1: ADAM12 transfected lysate (Predicted MW: 80.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ADAM12-Antibody-1G3-Western-Blot-H00008038-M01-img0003.jpg "Western Blot: ADAM12 Antibody (1G3) [H00008038-M01] - Analysis of ADAM12 expression in transfected 293T cell line by ADAM12 monoclonal antibody (M01), clone 1G3.Lane 1: ADAM12 transfected lysate (Predicted MW: 80.4 KDa).Lane 2: Non-transfected lysate.")
![ELISA: ADAM12 Antibody (1G3) [H00008038-M01] - Detection limit for recombinant GST tagged ADAM12 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ADAM12-Antibody-1G3-Sandwich-ELISA-H00008038-M01-img0002.jpg)
![Western Blot: ADAM12 Antibody [NBP1-59142] - Titration: 0.2-1 ug/ml, Positive Control: Human Placenta.](http://images.novusbio.com/fullsize/ADAM12-Antibody-Western-Blot-NBP1-59142-img0003.jpg "Western Blot: ADAM12 Antibody [NBP1-59142] - Titration: 0.2-1 ug/ml, Positive Control: Human Placenta.")
![Western Blot: ADAM12 Antibody (737) [NBP2-89702] - Lane A: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:100 kDa Observed band size:100 kDa](http://images.novusbio.com/fullsize/ADAM12-Antibody-737-Western-Blot-NBP2-89702-img0001.jpg "Western Blot: ADAM12 Antibody (737) [NBP2-89702] - Lane A: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:100 kDa Observed band size:100 kDa")