ADAM12 Antibody (1G3) - Azide and BSA Free

Western Blot: ADAM12 Antibody (1G3) [H00008038-M01] - Analysis of ADAM12 expression in transfected 293T cell line by ADAM12 monoclonal antibody (M01), clone 1G3.Lane 1: ADAM12 transfected lysate (Predicted MW: 80.4 KDa).Lane 2: Non-transfected lysate.

ELISA: ADAM12 Antibody (1G3) [H00008038-M01] - Detection limit for recombinant GST tagged ADAM12 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 1G3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ADAM12 Antibody (1G3) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ADAM12 (NP_003465, 208 a.a. ~ 304 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ETLKATKYVELVIVADNREFQRQGKDLEKVKQRLIEIANHVDKFYRPLNIRIVLVGVEVWNDMDKCSVSQDPFTSLHEFLDWRKMKLLPRKSHDNAQ
• Localization: Type I membrane protein (isoform 12L). A secreted form (isoform 12S) is produced by alternative splicing.
• Specificity: ADAM12 - ADAM metallopeptidase domain 12 (meltrin alpha)
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ADAM12
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ADAM12 Antibody (1G3) - Azide and BSA Free

• a disintegrin and metalloproteinase domain 12 (meltrin alpha)
• ADAM 12
• ADAM metallopeptidase domain 12
• ADAM12
• disintegrin and metalloproteinase domain-containing protein 12
• EC 3.4.24
• EC 3.4.24.58
• MCMP
• MCMPMltna
• Meltrin alpha
• meltrin-alpha
• MLTNA
• MLTNEC 3.4.24.-

Background

This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. The shorter form is found to stimulate myogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ADAM12 Antibody (H00008038-M01)(1)

Publication using H00008038-M01

• Zhou Z, Ran YL, Hu H et al. TM4SF3 promotes esophageal carcinoma metastasis via upregulating ADAM12m expression. Clin Exp Metastasis;25(5):537-48. 2008-01-01 [PMID: 18365756]

Bioinformatics

• Gene Symbol: ADAM12
• Entrez:
  • 8038(Human)
• OMIM:
  • 602714(Human)
• Uniprot:
  • NP_003465(Human)