Reactivity | MuSpecies Glossary |
Applications | WB, IHC |
Clonality | Polyclonal |
Host | Goat |
Conjugate | Unconjugated |
Concentration | LYOPH |
Immunogen | S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse ADAM10 Gly19-Glu673 Accession # O35598 |
Specificity | Detects mouse ADAM10 Ectodomain in Western blots. In Western blots (under non-reducing conditions), approximately 5% cross‑reactivity with recombinant human (rh) ADAM10 is observed. Does not cross-react with rhADAM9, rhADAM15, rhADAM8, and rhADAM17. |
Source | N/A |
Isotype | IgG |
Clonality | Polyclonal |
Host | Goat |
Gene | ADAM10 |
Purity Statement | Protein A or G purified |
Endotoxin Note | <0.10 EU per 1 μg of the antibody by the LAL method. |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Publications |
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Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Preservative | No Preservative |
Concentration | LYOPH |
Reconstitution Instructions | Reconstitute at 1 mg/mL in sterile PBS. |
ADAM10 (also known as Kuzbanian, mammalian disintegrin metalloprotease, myelin-associated metalloproteinase) is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). Like other membrane-anchored ADAMs, ADAM10 consists of the following domains, pro with a cysteine switch and furin cleavage sequence, catalytic with the zinc-binding site and Met-turn expected for reprolysins, disintegrin-like, cysteine-rich, EGF-like, transmembrane, and cytoplasmic. ADAM10 is highly conserved, with 97% amino acid identity between mouse, rat, bovine and human and 45% identity between mouse and Drosophila. The active enzyme processes notch, notch ligand delta, and amyloid protein precursor at the alpha site, playing an important role in neurogenesis (3, 4). It also processes the 26 kDa membrane-anchored pro-tumor necrosis factor-alpha (TNF-alpha ) to the 17 kDa mature TNF-alpha (5). It cleaves myelin basic protein and type IV collagen (6, 7). ADAM10 is widely expressed in tissues and resides both on the cell surface and in the cell (8, 9).
Secondary Antibodies |
Isotype Controls |
Diseases for ADAM10 Antibody (AB946)Discover more about diseases related to ADAM10 Antibody (AB946).
| Pathways for ADAM10 Antibody (AB946)View related products by pathway.
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PTMs for ADAM10 Antibody (AB946)Learn more about PTMs related to ADAM10 Antibody (AB946).
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