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ADAM10 Antibody [Unconjugated]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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ADAM10 Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse ADAM10
Gly19-Glu673
Accession # O35598
Specificity
Detects mouse ADAM10 Ectodomain in Western blots. In Western blots (under non-reducing conditions), approximately 5% cross‑reactivity with recombinant human (rh) ADAM10 is observed. Does not cross-react with rhADAM9, rhADAM15, rhADAM8, and rhADAM17.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ADAM10
Purity Statement
Protein A or G purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AB946 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 1 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADAM10 Antibody [Unconjugated]

  • a disintegrin and metalloprotease domain 10
  • a disintegrin and metalloproteinase domain 10
  • AD10
  • ADAM 10
  • ADAM metallopeptidase domain 10
  • ADAM10
  • CD156c antigen
  • CD156c
  • CDw156
  • disintegrin and metalloproteinase domain-containing protein 10
  • EC 3.4.24
  • HsT18717
  • kuz
  • Kuzbanian protein homolog
  • Kuzbanian
  • MADM
  • MADMEC 3.4.24.81
  • Mammalian disintegrin-metalloprotease

Background

ADAM10 (also known as Kuzbanian, mammalian disintegrin metalloprotease, myelin-associated metalloproteinase) is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). Like other membrane-anchored ADAMs, ADAM10 consists of the following domains, pro with a cysteine switch and furin cleavage sequence, catalytic with the zinc-binding site and Met-turn expected for reprolysins, disintegrin-like, cysteine-rich, EGF-like, transmembrane, and cytoplasmic. ADAM10 is highly conserved, with 97% amino acid identity between mouse, rat, bovine and human and 45% identity between mouse and Drosophila. The active enzyme processes notch, notch ligand delta, and amyloid protein precursor at the alpha site, playing an important role in neurogenesis (3, 4). It also processes the 26 kDa membrane-anchored pro-tumor necrosis factor-alpha (TNF-alpha ) to the 17 kDa mature TNF-alpha (5). It cleaves myelin basic protein and type IV collagen (6, 7). ADAM10 is widely expressed in tissues and resides both on the cell surface and in the cell (8, 9).

  1. Rooke, J. et al. (1996) Science 273:1227.
  2. Pan, D. and Rubin, G.M. (1997) Cell 90:271.
  3. Qi, H. et al. (1999) Science 283:91.
  4. Lammich, S. et al. (1999) Proc. Natl. Acad. Sci. USA 96:3922.
  5. Rosendahl, M.S. et al. (1997) J. Biol. Chem. 272:24588.
  6. Chantry, A. et al. (1989) J. Biol. Chem. 264:21603.
  7. Millichip, M.I. et al. (1998) Biochem. Biophys. Res. Comm. 245:594.
  8. Chantry, A. and Glynn, P. (1990) Biochem. J. 268:245.
  9. Fahrenholz, F.S. et al. (2000) Ann. N.Y. Acad. Sci. 920:215.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ADAM10 Antibody
[Unconjugated]
AB946
ADAM10 Antibody [Unconjugated]
Species: Mu
Applications: WB, IHC

Publications for ADAM10 Antibody (AB946)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC-P.


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(2)
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Bioinformatics

Gene Symbol ADAM10
Uniprot