ADAM10 Antibody [Unconjugated]

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

ADAM10 Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse ADAM10
  Gly19-Glu673
  Accession # O35598
• Specificity: Detects mouse ADAM10 Ectodomain in Western blots. In Western blots (under non-reducing conditions), approximately 5% cross‑reactivity with recombinant human (rh) ADAM10 is observed. Does not cross-react with rhADAM9, rhADAM15, rhADAM8, and rhADAM17.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ADAM10
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 1 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADAM10 Antibody [Unconjugated]

• a disintegrin and metalloprotease domain 10
• a disintegrin and metalloproteinase domain 10
• AD10
• ADAM 10
• ADAM metallopeptidase domain 10
• ADAM10
• CD156c antigen
• CD156c
• CDw156
• disintegrin and metalloproteinase domain-containing protein 10
• EC 3.4.24
• HsT18717
• kuz
• Kuzbanian protein homolog
• Kuzbanian
• MADM
• MADMEC 3.4.24.81
• Mammalian disintegrin-metalloprotease

Background

ADAM10 (also known as Kuzbanian, mammalian disintegrin metalloprotease, myelin-associated metalloproteinase) is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). Like other membrane-anchored ADAMs, ADAM10 consists of the following domains, pro with a cysteine switch and furin cleavage sequence, catalytic with the zinc-binding site and Met-turn expected for reprolysins, disintegrin-like, cysteine-rich, EGF-like, transmembrane, and cytoplasmic. ADAM10 is highly conserved, with 97% amino acid identity between mouse, rat, bovine and human and 45% identity between mouse and Drosophila. The active enzyme processes notch, notch ligand delta, and amyloid protein precursor at the alpha site, playing an important role in neurogenesis (3, 4). It also processes the 26 kDa membrane-anchored pro-tumor necrosis factor-alpha (TNF-alpha ) to the 17 kDa mature TNF-alpha (5). It cleaves myelin basic protein and type IV collagen (6, 7). ADAM10 is widely expressed in tissues and resides both on the cell surface and in the cell (8, 9).

1. Rooke, J. et al. (1996) Science 273:1227.
2. Pan, D. and Rubin, G.M. (1997) Cell 90:271.
3. Qi, H. et al. (1999) Science 283:91.
4. Lammich, S. et al. (1999) Proc. Natl. Acad. Sci. USA 96:3922.
5. Rosendahl, M.S. et al. (1997) J. Biol. Chem. 272:24588.
6. Chantry, A. et al. (1989) J. Biol. Chem. 264:21603.
7. Millichip, M.I. et al. (1998) Biochem. Biophys. Res. Comm. 245:594.
8. Chantry, A. and Glynn, P. (1990) Biochem. J. 268:245.
9. Fahrenholz, F.S. et al. (2000) Ann. N.Y. Acad. Sci. 920:215.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ADAM10 Antibody (AB946)(3)

Publications using AB946

• A Ikeda, S Shahid, BR Blumberg, M Suzuki, JD Bartlett ADAM10 is Expressed by Ameloblasts, Cleaves the RELT TNF Receptor Extracellular Domain and Facilitates Enamel Development Sci Rep, 2019-10-01;9(1):14086. 2019-10-01 [PMID: 31575895] (IHC-P, Mouse)
• A Disintegrin and Metalloproteinase10 (ADAM10) Regulates NOTCH Signaling during Early Retinal Development PLoS ONE, 2016-05-25;11(5):e0156184. 2016-05-25 [PMID: 27224017] (IHC-P, Mouse)
• Leann H. Brennaman, Marcia L. Moss, Patricia F. Maness EphrinA/EphA-induced ectodomain shedding of neural cell adhesion molecule regulates growth cone repulsion through ADAM10 metalloprotease Journal of Neurochemistry 1/1/2014 [PMID: 24117969]

Bioinformatics

• Gene Symbol: ADAM10
• Uniprot:
  • O35598()