ACTR1B Antibody (JE65-44)

Immunohistochemistry: ACTR1B Antibody (JE65-44) [NBP3-31999] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-ACTR1B antibody (NBP3-31999) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-31999) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: ACTR1B Antibody (JE65-44) [NBP3-31999] - Flow cytometric analysis of HepG2 cells labeling ACTR1B. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-31999, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Western Blot: ACTR1B Antibody (JE65-44) [NBP3-31999] - Western blot analysis of ACTR1B on HepG2 cell lysates with Rabbit anti-ACTR1B antibody (NBP3-31999) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-31999) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu
• Applications: WB, Flow, IHC
• Clone: JE65-44
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

ACTR1B Antibody (JE65-44) Summary

• Description: Novus Biologicals Rabbit ACTR1B Antibody (JE65-44) (NBP3-31999) is a recombinant monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within human ACTR1B aa 277-376/376. (Uniprot: P42025)
• Localization: Cytoskeleton, centrosome.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ACTR1B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:500-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:400
  • Western Blot 1:1000
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ACTR1B Antibody (JE65-44)

• Actin-related protein 1B
• ARP1 (actin-related protein 1, yeast) homolog B (centractin beta)
• ARP1 actin-related protein 1 homolog B, centractin beta (yeast)
• ARP1BPC3
• centractin beta
• CTRN2beta-centractin

Background

In addition to conventional actins, there are several actin-related proteins. The ACTR1B protein and the ACTR1A protein are of equal length, and they share 90% amino acid identity and 96% amino acid similarity. ACTR1B is expressed in the cytosol as part of the dynactin complex. The dynactin complex has been shown to localize to multiple structures within the cell, including membrane organelles, the centrosome, spindle poles, and spindle pole microtubules during mitosis and prometaphase kinetochores. It regulates dyneinmediated vesicle movement on microtubules as well as spindle assembly and cell division. ACTR1B appears to be ivolved in dynein-driven vesicle movement.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACTR1B