Activin RIIB Antibody

Immunohistochemistry-Paraffin: Activin RIIB Antibody [NBP2-15268] - Activin Receptor Type IIB antibody [N1C1] detects Activin Receptor Type IIB protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse placenta. Activin Receptor Type IIB stained by Activin Receptor Type IIB antibody [N1C1] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: Activin RIIB Antibody [NBP2-15268] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Activin Receptor Type IIB antibody [N1C1] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Activin Receptor Type IIB antibody [N1C1] (NBP2-15268) diluted at 1:25000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Activin Receptor Type IIB antibody [N1C1] (NBP2-15268) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Activin RIIB Antibody Summary

• Description: Novus Biologicals Rabbit Activin RIIB Antibody (NBP2-15268) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Activin RIIB. The exact sequence is proprietary.
• Predicted Species: Rat (99%), Porcine (100%), Bovine (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACVR2B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:50000
• Theoretical MW: 58 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Activin RIIB Antibody

• activin A receptor, type IIB
• Activin receptor type IIB
• activin receptor type-2B
• Activin RIIB
• ActivinRIIB
• ACTRIIB
• ActR-IIB
• ACVR2B
• EC 2.7.11
• EC 2.7.11.30
• MGC116908

Background

Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptors are considered to be constitutively active kinases. This gene encodes activin A type IIB receptor, which displays a 3- to 4-fold higher affinity for the ligand than activin A type II receptor. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACVR2B
• Uniprot:
  • Q13705()