Activin RIA/ALK-2/Activin Receptor Type 1 Antibody (2E2C11)

Western Blot: Activin RIA/ALK-2/Activin Receptor Type 1 Antibody (2E2C11) [NBP2-61799] - Analysis using ACVR1 mAb against human ACVR1 (AA: 21-120) recombinant protein. (Expected MW is 37.1 kDa)

Immunocytochemistry/Immunofluorescence: Activin RIA/ALK-2/Activin Receptor Type 1 Antibody (2E2C11) [NBP2-61799] - Analysis of Hela cells using ACVR1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 Secondary antibody was used.

Immunohistochemistry-Paraffin: Activin RIA/ALK-2/Activin Receptor Type 1 Antibody (2E2C11) [NBP2-61799] - Analysis of endometrial cancer tissues using ACVR1 mouse mAb with DAB staining.

Western Blot: Activin RIA/ALK-2/Activin Receptor Type 1 Antibody (2E2C11) [NBP2-61799] - Analysis using ACVR1 mAb against HEK293 (1) and ACVR1 (AA: 21-120)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: Activin RIA/ALK-2/Activin Receptor Type 1 Antibody (2E2C11) [NBP2-61799] - Analysis of Hela cells using ACVR1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Immunohistochemistry-Paraffin: Activin RIA/ALK-2/Activin Receptor Type 1 Antibody (2E2C11) [NBP2-61799] - Analysis of ovarian cancer tissues using ACVR1 mouse mAb with DAB staining.

ELISA: Activin RIA/ALK-2/Activin Receptor Type 1 Antibody (2E2C11) [NBP2-61799] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

ELISA: Activin RIA/ALK-2/Activin Receptor Type 1 Antibody (2E2C11) [NBP2-61799] - Analysis of Hela cells using ACVR1 mouse mAb (green) and negative control (red).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
• Clone: 2E2C11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Activin RIA/ALK-2/Activin Receptor Type 1 Antibody (2E2C11) Summary

• Immunogen: Purified recombinant fragment of human ACVR1 (AA: 21-120) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACVR1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
• Theoretical MW: 57.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for Activin RIA/ALK-2/Activin Receptor Type 1 Antibody (2E2C11)

• activin A receptor, type I
• Activin RIA
• ActivinRIA
• ACTRI
• ACVR1
• ACVR1A
• ACVRLK2
• ALK2
• ALK-2
• FOP
• SKR1
• TSRI

Background

Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I ( I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. This gene encodes activin A type I receptor which signals a particular transcriptional response in concert with activin type II receptors. Mutations in this gene are associated with fibrodysplasia ossificans progressive.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACVR1