actin-related protein 2/3 complex subunit 1B Antibody

Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NBP3-23370] - Western blot of rat A7r5 (lane 1), human Jurkat (lane 2), rat PC12 (lane 3), human PC3 (lane 4), mouse C2C12 (lane 5), and human A431 cells (lane 6). The blot was probed with rabbit polyclonal anti-Arpc1b (C-terminal region).

• Reactivity: Hu, Mu, Rt, Ch
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

actin-related protein 2/3 complex subunit 1B Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: actin-related protein 2/3 complex subunit 1B synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human actin-related protein 2/3 complex subunit 1B. This peptide sequence is highly conserved in rat, mouse, and chicken Arpc1b, and has some homology to the conserved region in isoform Arpc1a. [UniProt# O15143]
• Specificity: The antibody detects a 41 kDa protein corresponding to the molecular mass of actin-related protein 2/3 complex subunit 1B on SDS-PAGE immunoblots of human A431 and Jurkat cells, rat A7r5 and PC12 cells, and mouse C2C12 cells.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ARPC1B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Immunoprecipitation 1:100
  • Western Blot 1:1000
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for actin-related protein 2/3 complex subunit 1B Antibody

• actin related protein 2/3 complex, subunit 1B (41 kD)
• actin related protein 2/3 complex, subunit 1B, 41kDa
• actin-related protein 2/3 complex subunit 1B
• ARC41p41-ARCp40-ARC
• Arp2/3 complex 41 kDa subunit
• ARP2/3 protein complex subunit p41

Background

actin-related protein 2/3 complex subunit 1B encodes one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein encoded by gene ARPC1A. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ARPC1B