ACSBG1 Antibody (OTI1D8) - Azide and BSA Free

Western Blot: ACSBG1 Antibody (OTI1D8) - Azide and BSA Free [NBP2-71731] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACSBG1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACSBG1.

Immunocytochemistry/Immunofluorescence: ACSBG1 Antibody (OTI1D8) - Azide and BSA Free [NBP2-71731] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ACSBG1.

Immunohistochemistry: ACSBG1 Antibody (OTI1D8) - Azide and BSA Free [NBP2-71731] - Staining of paraffin-embedded liver tissue using anti-ACSBG1 mouse monoclonal antibody.

Immunohistochemistry: ACSBG1 Antibody (OTI1D8) - Azide and BSA Free [NBP2-71731] - Staining of paraffin-embedded Carcinoma of prostate tissue using anti-ACSBG1 mouse monoclonal antibody.

Immunohistochemistry: ACSBG1 Antibody (OTI1D8) - Azide and BSA Free [NBP2-71731] - Staining of paraffin-embedded colon tissue using anti-ACSBG1 mouse monoclonal antibody.

Immunohistochemistry: ACSBG1 Antibody (OTI1D8) - Azide and BSA Free [NBP2-71731] - Staining of paraffin-embedded Kidney tissue using anti-ACSBG1 mouse monoclonal antibody.

Immunohistochemistry: ACSBG1 Antibody (OTI1D8) - Azide and BSA Free [NBP2-71731] - Staining of paraffin-embedded prostate tissue using anti-ACSBG1 mouse monoclonal antibody.

Product Discontinued

ACSBG1 Antibody (OTI1D8) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human ACSBG1(NP_055977) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACSBG1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Western Blot 1:2000
• Theoretical MW: 81.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for ACSBG1 Antibody (OTI1D8) - Azide and BSA Free

• acyl-CoA synthetase bubblegum family member 1EC 6.2.1.3
• BG1
• BGMBG
• bubblegum
• FLJ30320
• hBG1
• hsBG
• hsBGM
• KIAA0631GR-LACS
• lipidosin
• long-chain-fatty-acid--CoA ligase ACSBG1
• LPD
• MGC14352
• very long-chain acyl-CoA synthetase

Background

ACSBG1 (Acyl CoA synthetase bubblegum family member 1) possesses long-chain acyl-CoA synthetase activity. It mediates activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. It is thought to play a central role in brain very long-chain fatty acids metabolism and myelinogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACSBG1