ACOT8 Antibody - Azide and BSA Free

Western Blot: ACOT8 Antibody [NBP2-92103] - Analysis of extracts of various cell lines, using ACOT8 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ACOT8 Antibody - Azide and BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ACOT8 (NP_005460.2). MSSPQAPEDGQGCGDRGDPPGDLRSVLVTTVLNLEPLDEDLFRGRHYWVPAKRLFGGQIVGQALVAAAKSVSEDVHVHSLHCYFVRAGDPKLPVLYQVERTRTGSSFSVRSVKAVQHGKPIFICQASFQQAQPSPMQHQFSMPTVPPPEELLDCETLIDQYLRDPNLQKRYPLALNRIAAQEVPIEIKPVNPSPLSQLQRMEPKQMFWVRARGYIGEGDM
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACOT8
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for ACOT8 Antibody - Azide and BSA Free

• ACTEIII
• acyl-CoA thioesterase 8HNAACTE
• Choloyl-coenzyme A thioesterase
• EC 3.1.2.27
• hACTEIII
• HIV-Nef-associated acyl-CoA thioesterase
• hTEcholoyl-CoA hydrolase
• long-chain fatty-acyl-CoA hydrolase
• palmitoyl-CoA hydrolase
• peroxisomal acyl-CoA thioesterase 1
• peroxisomal acyl-CoA thioesterase
• Peroxisomal acyl-coenzyme A thioester hydrolase 1
• Peroxisomal long-chain acyl-CoA thioesterase 1
• PTE-1
• PTE1HIV-Nef associated acyl-CoA thioesterase
• PTE2
• PTE-2hACTE-IIIacyl-coenzyme A thioesterase 8
• Thioesterase II
• thioesterase III

Background

ACOT8 is encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: ACOT8