Mouse ACF ELISA Kit (Colorimetric)

ELISA: Mouse ACF ELISA Kit (Colorimetric) [NBP2-66388] - Samples were spiked with high concentrations of Mouse ACF and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse ACF ELISA Kit (Colorimetric) [NBP2-66388] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 31.25 - 2000 pg/mL
• Sensitivity: 18.75 pg/mL

Mouse ACF ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse ACF in samples. No significant cross-reactivity or interference between Mouse ACF and analogues was observed.
• Standard Curve Range: 31.25 - 2000 pg/mL
• Sensitivity: 18.75 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.05%
• Intra-Assay: CV% < 5%
• Spike Recovery: 86-106%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: A1CF

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse ACF ELISA Kit (Colorimetric)

• ACF65
• ACFASPACF64
• apo-B RNA editing protein
• apobec-1 complementation factor (ACF) (ASP)
• APOBEC1 complementation factor
• APOBEC-1 stimulating protein
• APOBEC1CF
• APOBEC1-stimulating protein
• MGC163391

Background

Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events. Alternative splicing occurs at this locus and three full-length transcript variants, encoding three distinct isoforms, have been described. Additional splicing has been observed but the full-length nature of these variants has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: A1CF