Acetylcholinesterase/ACHE Antibody (4E11) - BSA Free

Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody (4E11) - BSA Free [NBP1-05146] - Human placenta stained with NBP1-05146

• Reactivity: Hu, Bv
• Applications: WB, ELISA, IHC
• Clone: 4E11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Acetylcholinesterase/ACHE Antibody (4E11) - BSA Free Summary

• Description: Novus Biologicals Mouse Acetylcholinesterase/ACHE Antibody (4E11) - BSA Free (NBP1-05146) is a monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: C-terminal 10 residues of brain acetylcholinesterase (human and bovine), absent from the erythrocyte enzyme.
• Epitope: C-terminal 10 residues (aa 574-583) of brain acetylcholinesterase (DS-AChE and SS-AChE) (1).
• Marker: Early Neuronal Development Marker
• Specificity: Specific for brain AChE and does not recognize AChE from erythrocytes. The can thus distinguish between mammalian brain AChE and erythrocyte AChE. Weak cross-reactivity with Torpedo marmorata AChE but none with AChE from electric eel or human BtChE.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACHE
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:75

Reactivity Notes

Human and Bovine.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for Acetylcholinesterase/ACHE Antibody (4E11) - BSA Free

• acetylcholinesterase (Yt blood group)
• Acetylcholinesterase
• ACHE
• apoptosis-related acetylcholinesterase
• ARACHE
• EC 3.1.1
• EC 3.1.1.7
• N-ACHE
• Yt blood group
• YT

Background

Acetylcholinesterase (AChE, EC.3.1.1.7.) is an enzyme located in the postsynaptic membrane and in the muscle endplates, where it hydrolyses the neurotransmitter acetylcholin. AChE from brain is a tetramer (G4-AChE) with a molecular mass of 320 kDa, AChE from erythrocytes is a dimer (G2-AChE) with a molecular mass of 170 kDa. Detection of higher levels of AChE in amniotic fluid can indicate fetal malformations such as neural tube defects.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACHE