ACAT2 Antibody (6Z5L1)

Western Blot: ACAT2 Antibody (6Z5L1) [NBP3-15476] - Western blot analysis of extracts of various cell lines, using ACAT2 antibody (NBP3-15476) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Western Blot: ACAT2 Antibody (6Z5L1) [NBP3-15476] - Western blot analysis of extracts of various cell lines, using ACAT2 antibody (NBP3-15476) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 6Z5L1
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

ACAT2 Antibody (6Z5L1) Summary

• Description: Novus Biologicals Rabbit ACAT2 Antibody (6Z5L1) (NBP3-15476) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 150-250 of human ACAT2 (Q9BWD1). GLTDAFHNCHMGITAENVAKKWQVSREDQDKVAVLSQNRTENAQKAGHFDKEIVPVLVSTRKGLIEVKTDEFPRHGSNIEAMSKLKPYFLTDGTGTVTPAN
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ACAT2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for ACAT2 Antibody (6Z5L1)

• acetoacetyl Coenzyme A thiolase
• acetyl-CoA acetyltransferase 2
• acetyl-CoA acetyltransferase, cytosolic
• Acetyl-CoA transferase-like protein
• acetyl-Coenzyme A acetyltransferase 2 (acetoacetyl Coenzyme A thiolase)
• acetyl-Coenzyme A acetyltransferase 2
• ACTL
• Cytosolic acetoacetyl-CoA thiolase
• EC 2.3.1
• EC 2.3.1.9

Background

The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACAT2