ACADL Antibody

Western Blot: ACADL Antibody [NBP2-15235] - Sample (30 ug of whole cell lysate) A: H1299 B: HCT116 10% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: ACADL Antibody [NBP2-15235] - Paraffin-embedded Hepatoma, using antibody at 1:500 dilution.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

ACADL Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human ACADL. The exact sequence is proprietary.
• Localization: Mitochondrion matrix
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACADL
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Porcine (85%), Bovine (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for ACADL Antibody

• ACAD4
• acyl-CoA dehydrogenase, long chain
• acyl-Coenzyme A dehydrogenase, long chain
• EC 1.3.99
• EC 1.3.99.13
• LCADFLJ94052
• long-chain specific acyl-CoA dehydrogenase, mitochondrial

Background

The protein encoded by this gene belongs to the acyl-CoA dehydrogenase family, which is a family of mitochondrial flavoenzymes involved in fatty acid and branched chain amino-acid metabolism. This protein is one of the four enzymes that catalyze the initial step of mitochondrial beta-oxidation of straight-chain fatty acid. Defects in this gene are the cause of long-chain acyl-CoA dehydrogenase (LCAD) deficiency, leading to nonketotic hypoglycemia. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ACADL
• Uniprot:
  • P28330(Human)