Abraxas Antibody - BSA Free

Western Blot: Abraxas Antibody [NBP1-76827] - Abraxas Antibody Human breast tissue lysate in (A) the absence and (B) presence of blocking peptide with CCDC98 antibody at 1 ug/ml.

Immunohistochemistry-Paraffin: Abraxas Antibody [NBP1-76827] - Abraxas Antibody Human breast tissue with FAM175A antibody at 5 ug/ml.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Abraxas Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Abraxas Antibody - BSA Free (NBP1-76827) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human CCDC98. The immunogen is located within amino acids 80 - 130 of CCDC98. Amino Acid Squence: WYKFRRHSDQIMTFR
• Predicted Species: Rat (100%), Bovine (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ABRAXAS1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1 ug/ml
• Control Peptide:

  Abraxas Peptide (NBP1-76827PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (87%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Abraxas Antibody - BSA Free

• BRCA1-A complex subunit Abraxas
• ABRA1
• CCDC98
• family with sequence similarity 175, member A

Background

CCDC98, also known as Abraxas 1, was identified through protein binding studies using the breast and ovarian predisposition protein BRCA1 as the binding target. CCDC98 recruits RAP80, a ubiquitin-binding protein, to BRCA1, allowing the formation of BRCA1 foci in response to DNA damage caused by ionizing radiation. Both CCDC98 and RAP80 are required for DNA damage resistance, G2-M checkpoint control, and DNA repair. Cells depleted of either CCDC98 or RAP80 exhibited increased sensitivity to ionizing radiation, although not as much as in BRCA1-depleted cells, suggesting that CCDC98 and RAP80 control only part of the DNA damage response role of BRCA1. At least two isoforms of CCDC98 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ABRAXAS1
• Uniprot:
  • NP_620775(Human)