BACH1 Antibody

Images

 
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and A172 human glioblastoma cell line. PVDF membrane was probed with 0.2 µg/mL of Human/Mouse BACH1 Antigen Affinity-purified Polyclonal ...read more
BACH1 was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Human/Mouse BACH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5776) at 10 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

BACH1 Antibody Summary

Immunogen
E. coli-derived recombinant human BACH1
Ser133-Glu513
Accession # O14867
Specificity
Detects human BACH1 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
BACH1
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.2 ug/mL
Publications
Read Publications using
AF5776 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BACH1 Antibody

  • BACH1
  • basic region leucine zipper transcriptional regulator BACH1
  • BTB and CNC homolog 1
  • BTB and CNC homology 1, basic leucine zipper transcription factor 1
  • BTB
  • HA2303
  • transcription regulator protein BACH1
  • transcription regulator protein10BACH-1

Background

BACH1 (BTB and CNC homolog 1; also basic leucine zipper transcription factor 1) is a ubiquitously expressed member of the Bach family of transcription factors. Although its predicted MW is 82 kDa, it runs anomalously at 110 kDa in SDS-PAGE. BACH1 forms non‑covalent homodimers, and heterodimers with Maf oncoproteins and p53-related proteins. It apparently serves as an architectural component for gene regulatory protein complexes. Human BACH1 is 736 amino acids (aa) in length. It contains a protein-interaction BTB domain (aa 24‑127), a DNA-binding motif (aa 562‑577), and a Leu-zipper domain (aa 585‑607). This molecule should not be confused with Bach1/Fancj/Brip1 helicase. There are two BACH1 splice variants with a 24 and 31 aa substitution for aa 593‑736, respectively. Over aa 133‑513, human BACH1 shares 75% aa identity with mouse BACH1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BACH1 Antibody (AF5776)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ChIP, Immunoprecipitation, Western Blot.


Filter By Application
ChIP
(1)
Immunoprecipitation
(2)
Western Blot
(2)
All Applications
Filter By Species
Human
(5)
All Species
Showing Publications 1 - 5 of 5.
Publications using AF5776 Applications Species
J Padilla, BS Lee, K Zhai, B Rentz, T Bobo, NM Dowling, J Lee A Heme-Binding Transcription Factor BACH1 Regulates Lactate Catabolism Suggesting a Combined Therapy for Triple-Negative Breast Cancer Cells, 2022;11(7):. 2022 [PMID: 35406740] (Immunoprecipitation, Human) Immunoprecipitation Human
Y Zhao, J Gao, X Xie, P Nan, F Liu, Y Sun, X Zhao BACH1 promotes the progression of esophageal squamous cell carcinoma by inducing the epithelial-mesenchymal transition and angiogenesis Cancer Medicine, 2021;0(0):. 2021 [PMID: 33932125] (ChIP, Human) ChIP Human
J Xu, H Guo, Z Xing, W Zhang, J He, J Cheng, R Cai Mild Oxidative Stress Reduces NRF2 SUMOylation to Promote Kras/Lkb1/Keap1 Mutant Lung Adenocarcinoma Cell Migration and Invasion Oxidative Medicine and Cellular Longevity, 2020;2020(0):6240125. 2020 [PMID: 33299528] (Western Blot, Human) Western Blot Human
X Wei, J Guo, Q Li, Q Jia, Q Jing, Y Li, B Zhou, J Chen, S Gao, X Zhang, M Jia, C Niu, W Yang, X Zhi, X Wang, D Yu, L Bai, L Wang, J Na, Y Zou, J Zhang, S Zhang, D Meng Bach1 regulates self-renewal and impedes mesendodermal differentiation of human embryonic stem cells Sci Adv, 2019;5(3):eaau7887. 2019 [PMID: 30891497] (Western Blot, Human) Western Blot Human
Attucks , Otis C, Jasmer , Kimberly, Hannink , Mark, Kassis , Jareer, Zhong , Zhenping, Gupta , Suparna, Victory , Sam F, Guzel , Mustafa, Polisetti , Dharma R, Andrews , Robert, Mjalli , Adnan M, Kostura , Matthew Induction of heme oxygenase I (HMOX1) by HPP-4382: a novel modulator of Bach1 activity. PLoS ONE, 2014;9(7):e101044. 2014 [PMID: 25019514] (Immunoprecipitation, Human) Immunoprecipitation Human

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Bioinformatics

Gene Symbol BACH1
Uniprot