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ABO, Blood Group A Antigen Antibody (HE-14)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IHC-P, Aggl, ICC/IF
Clone
HE-14
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-47868-0.1mg
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ABO, Blood Group A Antigen Antibody (HE-14) Summary

Immunogen
Mixture of erythrocytes of blood group A1 and glycoprotein fraction isolated from the saliva of secretors with blood group A
Localization
Cell Surface
Specificity
This antibody recognizes human blood group A (monofucosyl and difucosyl A antigens with chain types 1, 2, 3, 4, 5, 6) and Forssmann antigen. It is also reactive with the immuno-dominant A trisaccharide. Blood group antigen expression in human colon cancer was studied by means of two monoclonal antibodies of broad anti-A (HE-14) and anti-type 3 and type 4 chain-based A and H (HE-10) specificity. These antigens were proved to re-appear in tumors of the distal colon, the HE-10 antibody reacting more frequently (9 out of 12 samples) than HE-14 (5 out of 12 samples) and frequently with supra-nuclear staining of the cytoplasm probably in those places of the Golgi apparatus where carbohydrate antigens are synthesized. This staining pattern is characteristic of HE-10 in normal colonic mucosa as well. With HE-14, staining was often absent in less differentiated tumors, while HE-10 did react in such tumors. In some cases, these two antibodies gave different staining patterns in parallel sections from the same tissue sample, primarily at the cellular level. Three out of 12 cases showed blood group antigen expression in the mucosa of the distal colon adjacent to the tumor only when HE-10 Ab was used.
Isotype
IgM Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ABO
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • Agglutination
  • Immunofluorescence 0.5 - 1.0 ug/ml
Application Notes
Hu-chromosome location: 9q34.2
Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for ABO, Blood Group A Antigen Antibody (HE-14)

  • A transferase
  • A3GALNT
  • A3GALT1
  • ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase;transferase B, alpha 1-3-galactosyltransferase)
  • ABO glycosyltransferase
  • B transferase
  • B(A) alpha-1,3-galactosyltransferase
  • EC 2.4.1.37
  • EC 2.4.1.40
  • Fucosylglycoprotein 3-alpha-galactosyltransferase
  • Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase
  • Glycoprotein-fucosylgalactoside alpha-galactosyltransferase
  • Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase
  • GTB
  • Histo-blood group A transferase
  • histo-blood group A2 transferase
  • histo-blood group ABO system transferase
  • Histo-blood group B transferase
  • NAGAT

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ABO, Blood Group A
Antigen Antibody (HE- ...
NBP2-44629
ABO, Blood Group A Antigen Antibody (HE-14)
Species: Hu
Applications: IHC, IHC-P, Aggl, ICC/IF

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Bioinformatics

Gene Symbol ABO
Uniprot
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