ABL2 Antibody (6D5) - Azide and BSA Free

Western Blot: ABL2 Antibody (6D5) [H00000027-M03] - Analysis of ABL2 expression in transfected 293T cell line by ABL2 monoclonal antibody (M03), clone 6D5. Lane 1: ABL2 transfected lysatE (126.7 KDa). Lane 2: Non-transfected lysate.

Western Blot: ABL2 Antibody (6D5) [H00000027-M03] - Analysis of ABL2 over-expressed 293 cell line. Lane 2 cotransfected with ABL2 Validated Chimera RNAi Lane 1 non-transfected control. Blot probed with H00000027-M03. GAPDH ( 36.1 kDa ) used as loading control.

Western Blot: ABL2 Antibody (6D5) [H00000027-M03] - Analysis of ABL2 expression in Hela S3 NE (Cat # L013V3).

ELISA: ABL2 Antibody (6D5) [H00000027-M03] - Detection limit for recombinant GST tagged ABL2 is approximately 0.03ng/ml as a capture antibody.

Proximity Ligation Assay: ABL2 Antibody (6D5) [H00000027-M03] - Analysis of protein-protein interactions between NCK1 and ABL2. HeLa cells were stained with anti-NCK1 rabbit purified polyclonal 1:1200 and anti-ABL2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction com

• Reactivity: Hu
• Applications: WB, ELISA, Func, IP, KD
• Clone: 6D5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ABL2 Antibody (6D5) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ABL2 (AAH65912, 743 a.a. ~ 842 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KKTLGLRAGKPTASDDTSKPFPRSNSTSSMSSGLPEQDRMAMTLPRNCQRSKLQLERTVSTSSQPEENVDRANDMLPKKSEESAAPSRERPKAKLLPRGA
• Specificity: ABL2 (6D5)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ABL2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Knockdown Validated
  • Proximity Ligation Assay
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ABL2 Antibody (6D5) - Azide and BSA Free

• Abelson murine leukemia viral (v-abl) oncogene homolog 2
• Abelson murine leukemia viral oncogene homolog 2
• Abelson-related gene protein
• Abelson-relatedgene)
• ABL2
• ABLL
• arg tyrosine kinase
• ARG
• EC 2.7.10
• EC 2.7.10.2
• FLJ22224
• FLJ31718
• FLJ41441
• tyrosine-protein kinase ABL2
• Tyrosine-protein kinase ARG
• v-abl Abelson murine leukemia viral oncogene homolog 2

Background

The gene product is a cytoplasmic tyrosine kinase which is closely related to but distinct from ABL1. The similarity of the proteins includes the tyrosine kinase domains and extends amino-terminal to include the SH2 and SH3 domains. This gene is expressed in both normal and tumor cells. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ABL2 Antibody (H00000027-M03)(1)

Publication using H00000027-M03

• JP Hoj, B Mayro, AM Pendergast A TAZ-AXL-ABL2 Feed-Forward Signaling Axis Promotes Lung Adenocarcinoma Brain Metastasis Cell Rep, 2019-12-10;29(11):3421-3434.e8. 2019-12-10 [PMID: 31825826]

Bioinformatics

• Gene Symbol: ABL2
• Entrez:
  • 27(Human)
• OMIM:
  • 164690(Human)
• Uniprot:
  • AAH65912(Human)