Western Blot: Abhd5 Antibody [NB100-57850] - Mesenchymal stem cells differentiated into adipocytes. Antibody at 1:1000 in 5% BSA. Clear, strong band at expected molecular weight. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Abhd5 Antibody [NB100-57850] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton.Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 ...read more
Immunohistochemistry-Paraffin: Abhd5 Antibody [NB100-57850] - Staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: Abhd5 Antibody [NB100-57850] - Staining of NIH3T3 lysate (35 ug protein in RIPA buffer), antibody at 0.2 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Abhd5 Antibody [NB100-57850] - Staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western blot: Approx 40kDa band observed in lysates of cell line NIH3T3 (calculated MW of 39.1kDa according to NP_057090.2). Primary incubation was 1 hour. Additional faint bands of 26kDa and 18kDa were observed. These bands were successfully blocked by incubation with the immunizing peptide. IHC: Paraffin embedded Human Pancreas and Kidney.
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for Abhd5 Antibody
abhydrolase domain containing 5
Abhydrolase domain-containing protein 5,1-acylglycerol-3-phosphate O-acyltransferase ABHD5
Lipid droplet-binding protein CGI-58
The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated with Chanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.