Abhd5 Antibody

Western Blot: Abhd5 Antibody [NB100-57850] - Mesenchymal stem cells differentiated into adipocytes. Antibody at 1:1000 in 5% BSA. Clear, strong band at expected molecular weight. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: Abhd5 Antibody [NB100-57850] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton.Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and vesicle/ cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Immunohistochemistry-Paraffin: Abhd5 Antibody [NB100-57850] - Staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: Abhd5 Antibody [NB100-57850] - Staining of NIH3T3 lysate (35 ug protein in RIPA buffer), antibody at 0.2 ug/mL. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Abhd5 Antibody [NB100-57850] - Staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Abhd5 Antibody Summary

• Description: Novus Biologicals Goat Abhd5 Antibody (NB100-57850) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-Abhd5 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-FPERPDLADQDR corresponding to internal region according to NP_057090.2.
• Specificity: This antibody is not expected to cross-react with ABHD4.
• Predicted Species: Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ABHD5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunohistochemistry 3.75 ug/mL
  • Immunohistochemistry-Paraffin 3.75 ug/mL
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.2 - 0.6 ug/mL
• Application Notes: Western blot: Approx 40kDa band observed in lysates of cell line NIH3T3 (calculated MW of 39.1kDa according to NP_057090.2). Primary incubation was 1 hour. Additional faint bands of 26kDa and 18kDa were observed. These bands were successfully blocked by incubation with the immunizing peptide.
  IHC: Paraffin embedded Human Pancreas and Kidney.
• Control:

  A-431 Whole Cell Lysate
  Abhd5 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Abhd5 Antibody

• abhydrolase domain containing 5
• Abhydrolase domain-containing protein 5,1-acylglycerol-3-phosphate O-acyltransferase ABHD5
• CDS
• CGI58
• EC 2.3.1.51
• IECN2
• Lipid droplet-binding protein CGI-58
• MGC8731
• NCIE2CGI-58

Background

The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated with Chanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Abhd5 Antibody (NB100-57850)(3)

Publications using NB100-57850

• Anderson A, Lambert J, Montefusco D et al. Depletion of adipocyte sphingosine kinase 1 causes cell hypertrophy, impaired lipolysis, and NAFLD J Lipid Res. [PMID: 32690594] (WB, Mouse)
• Sitnick MT, Basantani MK, Cai L et al. Skeletal muscle triacylglycerol hydrolysis does not influence metabolic complications of obesity. Diabetes 2013-10-01 [PMID: 23835334] (ICC/IF, Mouse)
• Lass A, Zimmermann R, Haemmerle G et al. Adipose triglyceride lipase-mediated lipolysis of cellular fat stores is activated by CGI-58 and defective in Chanarin-Dorfman Syndrome. Cell Metab 2006-05-01 [PMID: 16679289]

Reviews for Abhd5 Antibody (NB100-57850) (3)

Average Rating: 5

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 12/06/2018

Summary

• Application: Western Blot
• Sample Tested: Mesenchymal stem cells differentiated into adipocytes
• Species: Mouse
• Lot: G2E250618

Comments

• Comments: PVDF membrane, 10% gel 180V 1hr; semi-dry transfer

reviewed by: Johana Lambert WB Mouse 11/16/2018

Summary

• Application: Western Blot
• Sample Tested: Mesenchymal stem cells differentiated into adipocytes
• Species: Mouse
• Lot: G2E250618

reviewed by: Verified Customer IF Mouse 01/15/2014

Summary

• Application: Immunofluorescence
• Species: Mouse

Bioinformatics

• Gene Symbol: ABHD5
• Entrez:
  • 51099(Human)
  • 67469(Mouse)
  • 316122(Rat)
• Uniprot:
  • Q8WTS1(Human)