Abhd5 Antibody

Images

 
Western Blot: Abhd5 Antibody [NB100-57850] - Mesenchymal stem cells differentiated into adipocytes. Antibody at 1:1000 in 5% BSA. Clear, strong band at expected molecular weight. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Abhd5 Antibody [NB100-57850] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton.Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 ...read more
Immunohistochemistry-Paraffin: Abhd5 Antibody [NB100-57850] - Staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: Abhd5 Antibody [NB100-57850] - Staining of NIH3T3 lysate (35 ug protein in RIPA buffer), antibody at 0.2 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Abhd5 Antibody [NB100-57850] - Staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Abhd5 Antibody Summary

Immunogen
Peptide with sequence C-FPERPDLADQDR corresponding to internal region according to NP_057090.2.
Specificity
This antibody is not expected to cross-react with ABHD4.
Predicted Species
Rat (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ABHD5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 - 0.6 ug/mL
  • Immunocytochemistry/Immunofluorescence 10 ug/mL
  • Immunohistochemistry 3.75 ug/mL
  • Immunohistochemistry-Paraffin 3.75 ug/mL
  • Peptide ELISA Detection limit 1:16000
Application Notes
Western blot: Approx 40kDa band observed in lysates of cell line NIH3T3 (calculated MW of 39.1kDa according to NP_057090.2). Primary incubation was 1 hour. Additional faint bands of 26kDa and 18kDa were observed. These bands were successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Pancreas and Kidney.
Control
A-431 Whole Cell Lysate
Abhd5 Overexpression Lysate
Reviewed Applications
Read 3 Reviews rated 5
using
NB100-57850 in the following applications:

Publications
Read Publications using
NB100-57850 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Abhd5 Antibody

  • abhydrolase domain containing 5
  • Abhydrolase domain-containing protein 5,1-acylglycerol-3-phosphate O-acyltransferase ABHD5
  • CDS
  • CGI58
  • EC 2.3.1.51
  • IECN2
  • Lipid droplet-binding protein CGI-58
  • MGC8731
  • NCIE2CGI-58

Background

The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated with Chanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF5365
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC
NB110-37253
Species: Hu, Mu, Rt
Applications: WB
NB110-40760
Species: Hu, Mu, Rt, Po, Fi
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-34294
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P
NB110-40877
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, Dual ISH-IHC
NBP1-88502
Species: Hu
Applications: IHC, IHC-P
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB110-60509
Species: Hu, Mu, Po, Bv
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-1269
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, PEP-ELISA
44110002
Species: Hu
Applications: WB, ELISA, ICC/IF
NBP1-82236
Species: Hu
Applications: WB, IHC, IHC-P, KD
NBP1-71690
Species: Hu
Applications: ICC/IF
NBP1-85691
Species: Hu
Applications: WB, Simple Western, IHC, IHC-P
NBP1-54611
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-49233
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-83950
Species: Hu
Applications: WB, IHC, IHC-P

Publications for Abhd5 Antibody (NB100-57850)(3)

Reviews for Abhd5 Antibody (NB100-57850) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Mouse.

Reviews using NB100-57850:
Filter by Applications
WB
(2)
IF
(1)
All Applications
Filter by Species
Mouse
(3)
All Species
Images Ratings Applications Species Date Details
Western Blot Abhd5 NB100-57850
Enlarge
reviewed by:
Anonymous
WB Mouse 12/06/2018
View

Summary

ApplicationWestern Blot
Sample TestedMesenchymal stem cells differentiated into adipocytes
SpeciesMouse
LotG2E250618

Comments

CommentsPVDF membrane, 10% gel 180V 1hr; semi-dry transfer
 
reviewed by:
Johana Lambert
WB Mouse 11/16/2018
View

Summary

ApplicationWestern Blot
Sample TestedMesenchymal stem cells differentiated into adipocytes
SpeciesMouse
LotG2E250618
 
reviewed by:
Anonymous
IF Mouse 01/15/2014
View

Summary

ApplicationImmunofluorescence
SpeciesMouse
Special ApplicationsAntibody works well wtih both immunoblotting and immunofluorescence imaging.
Pub Med ID23835334
FileView PDF

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Abhd5 Antibody (NB100-57850) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional Abhd5 Products

Bioinformatics Tool for Abhd5 Antibody (NB100-57850)

Discover related pathways, diseases and genes to Abhd5 Antibody (NB100-57850). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Abhd5 Antibody (NB100-57850)

Discover more about diseases related to Abhd5 Antibody (NB100-57850).
 

Pathways for Abhd5 Antibody (NB100-57850)

View related products by pathway.

PTMs for Abhd5 Antibody (NB100-57850)

Learn more about PTMs related to Abhd5 Antibody (NB100-57850).
 

Research Areas for Abhd5 Antibody (NB100-57850)

Find related products by research area.

Blogs on Abhd5

There are no specific blogs for Abhd5, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

Anonymous
12/06/2018
Application: WB
Species: Mouse

 
Johana Lambert
11/16/2018
Application: WB
Species: Mouse

 
Anonymous
01/15/2014
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol ABHD5
Entrez
Uniprot