ABAT Antibody - BSA Free

Western Blot: ABAT Antibody [NBP1-33066] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.

Western Blot: ABAT Antibody [NBP1-33066] - Hela cells

Western Blot: ABAT Antibody [NBP1-33066] - Rat liver lysate/extract 7. 5 % SDS-PAGE gel, antibody dilution 1:1000.

Immunohistochemistry-Paraffin: ABAT Antibody [NBP1-33066] - ABAT antibody detects ABAT protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. ABAT antibody diluted at 1:500.

Western Blot: ABAT Antibody [NBP1-33066] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ABAT antibody (NBP1-33066) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ABAT Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ABAT Antibody - BSA Free (NBP1-33066) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human ABAT. The exact sequence is proprietary.
• Localization: Mitochondrion matrix
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ABAT
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for ABAT Antibody - BSA Free

• (S)-3-amino-2-methylpropionate transaminase
• 4-aminobutyrate aminotransferase
• 4-aminobutyrate aminotransferase, mitochondrial
• 4-aminobutyrate transaminase
• ABAT
• EC 2.6.1.19
• EC 2.6.1.22
• FLJ17813
• FLJ30272
• G
• GABA aminotransferase
• GABA transaminase
• GABA transferase
• GABA-AT
• GABAT
• GABA-T
• GABATNPD009
• Gamma-amino-N-butyrate transaminase
• hCG1984265
• L-AIBAT

Background

4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ABAT