AATF [p Ser477] Antibody - Azide Free

Western Blot: AATF [p Ser477] Antibody [NBP2-29517] - Western blot of HeLa lysate showing specific immunolabeling of the ~66k Che-1 protein phosphorylated at Ser477. The phosphospecificity is shown in the second lane where immunoreactivity is blocked by preadsorption with the phospho-peptide (Peptide) used as antigen but not by the dephosphopeptide (not shown).

• Reactivity: Hu, Mu, Rt, Bv, Ca, Pm, Sh, Xp
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

AATF [p Ser477] Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit AATF [p Ser477] Antibody - Azide Free (NBP2-29517) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser477 conjugated to KLH. Accession # Q9NY61
• Modification: p Ser477
• Specificity: Specific for endogenous levels of the ~66 kDa AATF protein phosphorylated at Ser477. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
• Preparation Method: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Sheep (100%), Primate (100%), Xenopus (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AATF
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 66 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for AATF [p Ser477] Antibody - Azide Free

• apoptosis antagonizing transcription factor
• Apoptosis-antagonizing transcription factor
• CHE-1
• CHE1Rb-binding protein Che-1
• DEDprotein AATF

Background

Che-1, also known as AATF (apoptosis-antagonizing transcription factor), is a RNA polymerase II-binding protein involved in regulating the transcription factor E2F and promoting cell cycle progression (Burgdorf et al., 2004). It has been suggested that Che-1 may act as a neuroprotective factor against Abeta-induced apoptosis by suppressing the production of reactive oxidative species (Xie et al., 2004). The checkpoint kinase Chk2 has been shown to phosphorylate Che-1 at Ser477 contributing to the maintenance of the G2/M checkpoint induced by DNA damage (Bruno et al., 2006).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AATF
• Entrez:
  • 26574(Human)
• Uniprot:
  • Q9NY61(Human)