AADACL1 Antibody - BSA Free

Staining of human cell line A-431 shows positivity in cytoplasm & the Golgi apparatus.

Analysis in human cerebral cortex and skin tissues using NBP1-90294 antibody. Corresponding NCEH1 RNA-seq data are presented for the same tissues.

Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

Staining of human skin shows no positivity in squamous epithelial cells as expected.

Staining of human lung shows moderate cytoplasmic positivity in macrophages.

Staining of human tonsil shows strong cytoplasmic positivity in germinal center cells.

Staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.

Analysis in human cell line CAPAN-2.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

AADACL1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit AADACL1 Antibody - BSA Free (NBP1-90294) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-AADACL1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: ALIYPVLQALDFNTPSYQQNVNTPILPRYVMVKYWVDYFKGNYDFVQAMIVNNHTSLDVEEAAAVRARLNWTSLLPASFTKNYKPVVQTTGNARIVQELPQLLDARSAPLIADQAVLQLLPKTYILTCEHDVL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NCEH1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  AADACL1 Protein (NBP1-90294PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (83%). Reactivity reported in scientific literature (PMID: 22354961)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for AADACL1 Antibody - BSA Free

• AADACL1
• EC 3.1.1.-
• EC 3.1.1.71
• EC 3.1.1.79
• KIAA1363
• KIAA1363EC 3.1.1
• NCEH
• NCEH1
• NCEHArylacetamide deacetylase-like 1EC 3.1.1.-
• neutral cholesterol ester hydrolase 1

Background

Hydrolyzes 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. May be responsible for cholesterol ester hydrolysis in macrophages, thereby contributing to the development of atherosclerosis. Also involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds. May contribute to cancer pathogenesis by promoting tumor cell migration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AADACL1 Antibody (NBP1-90294)(1)

Publication using NBP1-90294

• Mei S, Gu H, Ward A et al. p38 Mitogen-activated Protein Kinase (MAPK) Promotes Cholesterol Ester Accumulation in Macrophages through Inhibition of Macroautophagy. J Biol Chem 2012-04-06 [PMID: 22354961]

Bioinformatics

• Gene Symbol: NCEH1