A4GNT Antibody [Unconjugated]

Western blot shows lysates of SW480 human colorectal adenocarcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, KATO-III human gastric carcinoma cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human a-1,4-N-Acetylglucosaminyltransferase 4/A4GNT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8555) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for a-1,4-N-Acetylglucosaminyltransferase 4/A4GNT at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

A4GNT Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human alpha -1,4-N-Acetylglucosaminyltransferase 4/A4GNT
  Leu25-Lys340
  Accession # Q9UNA3
• Specificity: Detects human alpha -1,4-N-Acetylglucosaminyltransferase in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: A4GNT
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000 dilution

Packaging, Storage & Formulations

• Storage:
  • 12 months from date of receipt, -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 °C under sterile conditions after opening.
• Buffer: Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide.
• Preservative: Sodium Azide

Notes

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for A4GNT Antibody [Unconjugated]

• A4GNT
• alpha-1,4-N-acetylglucosaminyltransferase
• alpha4GnT

Background

Alpha-1,4-N-Acetylglucosaminyltransferase 4, also known as A4GNT is a 45 kDa glycoprotein, member of the  glycosyltransferase 32 family of proteins. A4GNT catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. A4GNT is a single-pass type II membrane protein associated with the Golgi apparatus and contains the conserved DXD motif involved in catalytic activity. It is expressed in stomach and pancreas, as well as in gastric cancer cells. A4GNT is not expressed in peripheral blood cells, making it a putative biomarker for pancreatic cancer. A4GNT and Mucin 6 expression is upregulated in the gastric mucosa of H. pylori infected patients. Lumenal domain of human A4GNT shares 72% aa sequence identity with mouse and rat A4GNT.

 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: A4GNT
• Uniprot:
  • Q9UNA3()