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GCNT3 Antibody

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Immunohistochemistry-Paraffin: GCNT3 Antibody [NBP1-88313] - Staining in human rectum and liver tissues using anti-GCNT3 antibody. Corresponding GCNT3 RNA-seq data are presented for the same tissues.
Orthogonal Strategies: Immunohistochemistry-Paraffin: GCNT3 Antibody [NBP1-88313] - Staining in human rectum and liver tissues using anti-GCNT3 antibody. Corresponding GCNT3 RNA-seq data are presented for the ...read more
Western Blot: GCNT3 Antibody [NBP1-88313] - Analysis in human cell line A-549.
Immunocytochemistry/ Immunofluorescence: GCNT3 Antibody [NBP1-88313] - Immunofluorescent staining of human cell line A549 shows localization to the Golgi apparatus.
Immunohistochemistry-Paraffin: GCNT3 Antibody [NBP1-88313] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: GCNT3 Antibody [NBP1-88313] - Staining of human rectum shows high expression.
Simple Western: GCNT3 Antibody [NBP1-88313] - Simple Western lane view shows a specific band for GCNT3 in 0.5 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Simple Western: GCNT3 Antibody [NBP1-88313] - Simple Western lane view shows a specific band for GCNT3 in 0.5 mg/ml of U-251MG lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Simple Western: GCNT3 Antibody [NBP1-88313] - Electropherogram image(s) of corresponding Simple Western lane view. GCNT3 antibody was used at 1:20 dilution on RT-4 lysates(s).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

  

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GCNT3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: RLKCDSDHLGLESRESQSQYCRNILYNFLKLPAKRSINCSGVTRGDQEAVLQAILNNLEVKKKREPFTDTHYLSLTRDCEHFKAERKFIQFPLSKEEVEFPIAYSMVIHEKIENFER
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GCNT3
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Simple Western 1:20
  • Western Blot 0.04-0.4 ug/ml
Application Notes
IHC reported in scientific literature (PMID: 24889196). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
GCNT3 Protein (NBP1-88313PEP)
Publications
Read Publication using
NBP1-88313 in the following applications:

  • IHC
    1 publication

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24889196).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for GCNT3 Antibody

  • beta-1,3-galactosyl-O-glycosyl-glycoproteinbeta-1,6-N-acetylglucosaminyltransferase 3
  • beta1,6GlcNAc-transferase
  • beta1,6-N-acetylglucosaminyltransferase-M
  • C2/4GnTGNTM
  • C24GNT
  • C2GnT2
  • C2GNTM
  • C2GnT-M
  • C2GnT-mucin type
  • Core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase
  • EC 2.4.1.102
  • EC 2.4.1.150
  • glucosaminyl (N-acetyl) transferase 3, mucin type
  • hC2GnT-M
  • mucus-type core 2 beta-1,6-N-acetylglucosaminyltransferase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Glucosaminyl (N-acetyl) Transferase 1/GCNT1 was detected in immersion fixed HT‑29 human colon adenocarcinoma cell line using Sheep Anti-Human Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7248) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Western blot shows lysates of SW480 human colorectal adenocarcinoma cell line, TT human medullary thyroid cancer cell line, and Capan‑1 human pancreatic adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Glucosaminyl (N‑acetyl) Transferase 1/GCNT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7248) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Glucosaminyl (N-acetyl) Transferase 1/GCNT1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Species: Hu
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TNF-alpha
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210-TA
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
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Western Blot: FOXA3 Antibody [NBP2-87461] - WB Suggested Anti-FOXA3 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate
Western Blot: FOXA3 Antibody [NBP2-87461] - Host: Rabbit. Target Name: FOXA3. Sample Tissue: Mouse Skeletal Muscle. Antibody Dilution: 1ug/ml
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Immunohistochemistry-Paraffin: C2GNT3 Antibody [NBP1-88270] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Immunocytochemistry/Immunofluorescence: C2GNT3 Antibody [NBP1-88270] - Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & the Golgi apparatus.
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Flow Cytometry: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of Hela cells with hnRNP C1+C2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Staining hnRNP C1+C2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Staining hnRNP C1+C2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Flow Cytometry: MUC5AC Antibody (45M1) [NBP2-15196] - Analysis using the Azide and BSA Free version of NBP2-15196. Staining of fixed (70% ethanol or 80% methanol) and permeabilized A549 cells
Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196] - Analysis using the Azide and BSA Free version of NBP2-15196. Detection Human stomach.
Flow (Intracellular): MUC5AC Antibody (45M1) [NBP2-15196] - An intracellular stain was performed on A549 cells with Mucin 5AC (45M1) antibody NBP2-15196 (blue) and a matched isotype control NBP2-27287 (orange). To block the secretion of Mucin 5AC, cells were treated with 3uM Monensin for 3 hours. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).
(5 Reviews)
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Species: Bv(-), Ch, Fe, Hu, Ma, Pm, Mu, Po, Rb, Rt
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MUC2 Antibody
(996/1)
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Flow Cytometry: MUC2 Antibody (996/1) [NB120-11197] - Analysis of Allophycocyanin conjugate of NB120-11197. An intracellular stain was performed on NTERA-2 cells with MUC2 (996/1) antibody NB120-11197APC (blue) and a matched isotype control NBP2-27287APC (orange). Cells were fixed with 4% PFA and then permea
Flow (Intracellular): MUC2 Antibody (996/1) [NB120-11197] - An intracellular stain was performed on HeLa cells with MUC2 Antibody (996/1) NB120-11197AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow Cytometry: MUC2 Antibody (996/1) [NB120-11197] - An intracellular stain was performed on HeLa cells with MUC2 Antibody [996/1] NB120-11197AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
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Complement
Component C2b Antibod ...
MAB1936
Complement Component C2b Antibody (269716)
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Immunohistochemistry-Paraffin: GCNT2 Antibody [NBP1-88781] - Staining of human small intestine shows granular cytoplasmic positivity in glandular cells.
Western Blot: GCNT2 Antibody [NBP1-88781] - Analysis in human cell lines Caco-2 and MCF-7 using anti-GCNT2 antibody. Corresponding GCNT2 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
Immunohistochemistry-Paraffin: GCNT2 Antibody [NBP1-88781] - Staining of human Duodenum shows strong positivity in Golgi apparatus in glandular cells.
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Flow (Intracellular): DGAT2 Antibody [NBP3-05769] - An intracellular stain was performed on HepG2 cells with DGAT2 Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).
Flow (Intracellular): DGAT2 Antibody [NBP3-05769] - An intracellular stain was performed on NIH3T3 cells with DGAT2 Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).
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AF5128
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PLC-gamma 1
Antibody (3C2D11)
NBP2-52532
ELISA: PLC-gamma 1 Antibody (3C2D11) [NBP2-52532] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).
Flow Cytometry: PLC-gamma 1 Antibody (3C2D11) [NBP2-52532] - Analysis of Jurkat cells using PLCG1 mouse mAb (green) and negative control (red).
Immunocytochemistry/Immunofluorescence: PLC-gamma 1 Antibody (3C2D11) [NBP2-52532] - Analysis of HeLa cells using PLCG1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
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CD43/Sialophorin
Antibody (W3/13)
NB100-64992
Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (W3/13) [NB100-64992] - CD43 was detected in immersion fixed paraffin-embedded sections of rat thymus using Mouse Anti-Rat CD43 Monoclonal Antibody (Catalog # NB100-64992) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was incubated with VisUBlock™ Mouse on Mouse Blocking Reagent (Catalog # VB001) and then subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes.
Western Blot: CD43/Sialophorin Antibody (W3/13) [NB100-64992] - Western blot shows lysates of rat lymph node, rat spleen tissue, and rat thymus tissue. PVDF membrane was probed with 0.5 ug/mL of Mouse Anti-Rat CD43 Monoclonal Antibody (Catalog # NB100-64992) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD43 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions.
Flow Cytometry: CD43/Sialophorin Antibody (W3/13) [NB100-64992] - Staining of rat splenocytes using CD43 FITC.
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Western Blot: SPDEF Antibody [NBP3-04686] - Analysis of extracts of rat ovary, using SPDEF antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit .
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GCNT3 Antibody
NBP1-88313
GCNT3 Antibody
Species: Hu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P

Publications for GCNT3 Antibody (NBP1-88313)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC.


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Bioinformatics

Gene Symbol GCNT3