GCNT3 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: GCNT3 Antibody [NBP1-88313] - Immunofluorescent staining of human cell line A549 shows localization to the Golgi apparatus.

Immunohistochemistry-Paraffin: GCNT3 Antibody [NBP1-88313] - Staining in human rectum and liver tissues using anti-GCNT3 antibody. Corresponding GCNT3 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: GCNT3 Antibody [NBP1-88313] - Staining of human rectum shows high expression.

Immunohistochemistry-Paraffin: GCNT3 Antibody [NBP1-88313] - Staining of human liver shows low expression as expected.

Analysis in human cell line A-549.

Simple Western: GCNT3 Antibody [NBP1-88313] - Simple Western lane view shows a specific band for GCNT3 in 0.5 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: GCNT3 Antibody [NBP1-88313] - Simple Western lane view shows a specific band for GCNT3 in 0.5 mg/ml of U-251MG lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: GCNT3 Antibody [NBP1-88313] - Electropherogram image(s) of corresponding Simple Western lane view. GCNT3 antibody was used at 1:20 dilution on RT-4 lysates(s).

• Reactivity: Hu
• Applications: WB, Simple Western, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GCNT3 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: RLKCDSDHLGLESRESQSQYCRNILYNFLKLPAKRSINCSGVTRGDQEAVLQAILNNLEVKKKREPFTDTHYLSLTRDCEHFKAERKFIQFPLSKEEVEFPIAYSMVIHEKIENFER
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GCNT3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Simple Western 1:20
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: IHC reported in scientific literature (PMID: 24889196). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Use PFA/Triton X-100.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 and U-251MG, separated by Size, antibody dilution of 1:20, apparent MW was 61-64 kDa
• Control Peptide:

  GCNT3 Protein (NBP1-88313PEP)

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24889196).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for GCNT3 Antibody - BSA Free

• beta-1,3-galactosyl-O-glycosyl-glycoproteinbeta-1,6-N-acetylglucosaminyltransferase 3
• beta1,6GlcNAc-transferase
• beta1,6-N-acetylglucosaminyltransferase-M
• C2/4GnTGNTM
• C24GNT
• C2GnT2
• C2GNTM
• C2GnT-M
• C2GnT-mucin type
• Core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase
• EC 2.4.1.102
• EC 2.4.1.150
• glucosaminyl (N-acetyl) transferase 3, mucin type
• hC2GnT-M
• mucus-type core 2 beta-1,6-N-acetylglucosaminyltransferase

Background

GCNT3 catalyzes O-glycan branch synthesis of the core 2 and core 4 type in mucins and controls expression of core 2 branched oligosaccharides and I antigens on the cell surface. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GCNT3 Antibody (NBP1-88313)(1)

Publication using NBP1-88313

• van der Post S, Hansson GC. Membrane Protein Profiling of Human Colon Reveals Distinct Regional Differences. Mol Cell Proteomics 2014-09-01 [PMID: 24889196] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: GCNT3