![Immunohistochemistry-Paraffin: A33/GPA33 Antibody (071) [NBP2-89873] - Staining of human GPA33 in human colon carcinoma with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize2/A33-GPA33-Antibody-(071)-Immunohistochemistry-Paraffin-NBP2-89873-img0003.jpg "Immunohistochemistry-Paraffin: A33/GPA33 Antibody (071) [NBP2-89873] - Staining of human GPA33 in human colon carcinoma with rabbit monoclonal antibody (1:200).")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, IHC, IHC-P |
| Clone | 071 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | This antibody was obtained from a rabbit immunized with purified, recombinant Human A33/GPA33 (Q99795-1; Met1-Val235). |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | GPA33 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um filtered solution in PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-89873AF350 | |
| Alexa Fluor 405 | NBP2-89873AF405 | |
| Alexa Fluor 488 | NBP2-89873AF488 | |
| Alexa Fluor 532 | NBP2-89873AF532 | |
| Alexa Fluor 594 | NBP2-89873AF594 | |
| Alexa Fluor 647 | NBP2-89873AF647 | |
| Alexa Fluor 700 | NBP2-89873AF700 | |
| Alexa Fluor 750 | NBP2-89873AF750 | |
| Biotin | NBP2-89873B | |
| DyLight 350 | NBP2-89873UV | |
| DyLight 405 | NBP2-89873V | |
| DyLight 488 | NBP2-89873G | |
| DyLight 550 | NBP2-89873R | |
| DyLight 594 | NBP2-89873DL594 | |
| DyLight 650 | NBP2-89873C | |
| DyLight 680 | NBP2-89873FR | |
| DyLight 755 | NBP2-89873IR | |
| FITC | NBP2-89873F | |
| HRP | NBP2-89873H | |
| Janelia Fluor 549 | NBP2-89873JF549 | |
| Janelia Fluor 646 | NBP2-89873JF646 |
Diseases for A33/GPA33 Antibody (NBP2-89873)Discover more about diseases related to A33/GPA33 Antibody (NBP2-89873).
| Pathways for A33/GPA33 Antibody (NBP2-89873)View related products by pathway.
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PTMs for A33/GPA33 Antibody (NBP2-89873)Learn more about PTMs related to A33/GPA33 Antibody (NBP2-89873).
| Research Areas for A33/GPA33 Antibody (NBP2-89873)Find related products by research area.
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| Gene Symbol | GPA33 |
![Immunohistochemistry-Paraffin: A33/GPA33 Antibody (071) [NBP2-89873] - Staining of human GPA33 in human small intestine with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize2/A33-GPA33-Antibody-(071)-Immunohistochemistry-Paraffin-NBP2-89873-img0001.jpg "Immunohistochemistry-Paraffin: A33/GPA33 Antibody (071) [NBP2-89873] - Staining of human GPA33 in human small intestine with rabbit monoclonal antibody (1:200).")
![Immunohistochemistry-Paraffin: A33/GPA33 Antibody (071) [NBP2-89873] - Staining of human GPA33 in human colon with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize2/A33-GPA33-Antibody-(071)-Immunohistochemistry-Paraffin-NBP2-89873-img0002.jpg "Immunohistochemistry-Paraffin: A33/GPA33 Antibody (071) [NBP2-89873] - Staining of human GPA33 in human colon with rabbit monoclonal antibody (1:200).")
![Western Blot: EIF3M Antibody [NBP1-83552] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/images2/EIF3M-Antibody-Western-Blot-NBP1-83552-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: EIF3M Antibody [NBP1-83552] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/images2/EIF3M Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83552-img0008.jpg)
![Immunohistochemistry-Paraffin: EIF3M Antibody [NBP1-83552] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/images2/EIF3M-Antibody-Immunohistochemistry-Paraffin-NBP1-83552-img0006.jpg)
![Western Blot: STT3A Antibody (4D4) [H00003703-M02] - Analysis of ITM1 expression in transfected 293T cell line by ITM1 monoclonal antibody (M02), clone 4D4.Lane 1: ITM1 transfected lysate(80.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/images2/STT3A-Antibody-(4D4)-Western-Blot-H00003703-M02-img0006.jpg)
![Immunohistochemistry-Paraffin: STT3A Antibody (4D4) [H00003703-M02] - Analysis of monoclonal antibody to ITM1 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml.](http://images.novusbio.com/images2/STT3A-Antibody-(4D4)-Immunohistochemistry-Paraffin-H00003703-M02-img0004.jpg)
![ELISA: STT3A Antibody (4D4) [H00003703-M02] - Detection limit for recombinant GST tagged ITM1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/images2/STT3A-Antibody-(4D4)-ELISA-H00003703-M02-img0007.jpg)
![BMP-4 [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "BMP-4 [Unconjugated]")
![Western Blot: HLA DRB4 Antibody (4C8) [H00003126-M01] - HLA-DRB4 monoclonal antibody (M01), clone 4C8. Analysis of HLA-DRB4 expression in human kidney.](http://images.novusbio.com/images2/HLA-DRB4-Antibody-(4C8)-Western-Blot-H00003126-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: HLA DRB4 Antibody (4C8) [H00003126-M01] - Analysis of monoclonal antibody to HLA-DRB4 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 1 ug/ml.](http://images.novusbio.com/images2/HLA-DRB4-Antibody-(4C8)-Immunohistochemistry-Paraffin-H00003126-M01-img0004.jpg)
![Sandwich ELISA: HLA DRB4 Antibody (4C8) [H00003126-M01] - Detection limit for recombinant GST tagged HLA-DRB4 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/images2/HLA-DRB4-Antibody-(4C8)-Sandwich-ELISA-H00003126-M01-img0005.jpg)
![Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Analysis using the FITC conjugate of NB100-64775. Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control FITC (NBP1-43955) (blue histogram) or Anti-Human HLA-ABC FITC (purple histogram). Cells in the lymphocyte gate were used f](http://images.novusbio.com/images2/HLA-ABC-Antibody-(W6-32)-Flow-Cytometry-NB100-64775-img0013.jpg)
![Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC.](http://images.novusbio.com/images2/HLA-ABC-Antibody-(W6-32)-Flow-Cytometry-NB100-64775-img0002.jpg)
![Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:Alexa Fluor 488.](http://images.novusbio.com/images2/HLA-ABC-Antibody-(W6-32)-Flow-Cytometry-NB100-64775-img0004.jpg)
![Western Blot: FAM49B Antibody [NBP1-88582] - Analysis in human cell line A-431.](http://images.novusbio.com/images2/FAM49B-Antibody-Western-Blot-NBP1-88582-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: FAM49B Antibody [NBP1-88582] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.](http://images.novusbio.com/images2/FAM49B Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88582-img0008.jpg)
![Immunohistochemistry-Paraffin: FAM49B Antibody [NBP1-88582] - Staining of human lymph node shows strong cytoplasmic positivity in germinal center cells and non-germinal center cells.](http://images.novusbio.com/images2/FAM49B-Antibody-Immunohistochemistry-Paraffin-NBP1-88582-img0007.jpg)
![Western Blot: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of alpha sarcoglycan on rat heart tissue lysates using anti-alpha sarcoglycan antibody at 1:500 dilution.](http://images.novusbio.com/images2/alpha-Sarcoglycan Antibody (JA51-81)-Western Blot-NBP2-67150-img0003.jpg)
![Immunohistochemistry-Frozen: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Human skeletal muscle tissue. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/images2/alpha-Sarcoglycan-Antibody-(JA51-81)-Immunohistochemistry-Frozen-NBP2-67150-img0005.jpg)
![Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of paraffin-embedded mouse heart tissue using anti-alpha sarcoglycan antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/alpha-Sarcoglycan-Antibody-(JA51-81)-Immunohistochemistry-Paraffin-NBP2-67150-img0001.jpg)
![Western Blot: GPHA2 Antibody [NBP3-04883] - Analysis of extracts of rat brain, using GPHA2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.](http://images.novusbio.com/images2/GPHA2 Antibody-Western Blot-NBP3-04883-img0001.jpg)
![Western Blot: ATP6V0A2 Antibody [NBP1-59069] - HeLa cells, concentration 3.3 ug/ml.](http://images.novusbio.com/images2/ATP6V0A2-Antibody-Western-Blot-NBP1-59069-img0002.jpg)
![Immunohistochemistry: ATP6V0A2 Antibody [NBP1-59069] - Human kidney lysate tissue at an antibody concentration of 5.0 ug/ml using anti-ATP6V0A2 antibody](http://images.novusbio.com/images2/ATP6V0A2-Antibody-Immunohistochemistry-NBP1-59069-img0008.jpg)
![Western Blot: ATP6V0A2 Antibody [NBP1-59069] - Hela tissue lysate at a concentration of 1ug/ml.](http://images.novusbio.com/images2/ATP6V0A2-Antibody-Western-Blot-NBP1-59069-img0001.jpg)
![Western Blot: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis using HLA-B mAb against HEK293 (1) and HLA-B (AA: 241-362)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/images2/HLA-B-Antibody-(2G7B10)-Western-Blot-NBP2-61871-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis of Hela cells using HLA-B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/images2/HLA-B-Antibody-(2G7B10)-Immunocytochemistry-Immunofluorescence-NBP2-61871-img0004.jpg)
![Immunohistochemistry-Paraffin: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis of bladder cancer tissues using HLA-B mouse mAb with DAB staining.](http://images.novusbio.com/images2/HLA-B-Antibody-(2G7B10)-Immunohistochemistry-NBP2-61871-img0006.jpg)
![Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Analysis of a FFPE human breast carcinoma tissue section using 1:100 dilution of EpCAM/TROP1 antibody (clone AUA1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This EpCAM antibody generated an expected immunostaining of EpCAM (CD326) protein in the membranes of the cancer cells and the inter-cellular spaces. The tumor stroma and the stromal cells did not show EpCAM immunopositivity.](http://images.novusbio.com/images2/EpCAM-TROP1-Antibody-(AUA1)-Immunohistochemistry-Paraffin-NB600-1182-img0009.jpg)
![Western Blot: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Detection of EpCAM/CD326 in two Human mammary tumor cell lines, MDA-MB-231 (mesenchyme-like) and MCF-7 (epithelium-like). Dilution: 1:500 in PBS with 5% BSA. Secondary Ab: anti-Mouse IgG 1:5,000. This image was submitted via customer Review.](http://images.novusbio.com/images2/EpCAM-TROP1-Antibody-(AUA1)-Western-Blot-NB600-1182-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-EpCAM/TROP1 (AUA1) conjugated to Alexa Fluor 488 [NB600-1182AF488] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/EpCAM-TROP1-Antibody-(AUA1)-Immunocytochemistry-Immunofluorescence-NB600-1182-img0016.jpg)
![Western Blot: VSIG1 Antibody [NBP1-81074] - Analysis in control (vector only transfected HEK293T lysate) and vSIG1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/VSIG1-Antibody-Western-Blot-NBP1-81074-img0006.jpg)
![Immunohistochemistry-Paraffin: VSIG1 Antibody [NBP1-81074] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/images2/VSIG1-Antibody-Immunohistochemistry-Paraffin-NBP1-81074-img0007.jpg)
![Western Blot: VSIG1 Antibody [NBP1-81074] - Analysis in denatured VSIG1 overexpression lysate (Cat. # H00340547-T01, left), vector only transfected lysate (center) and native VSIG1 overexpression lysate (Cat. # NBL1-17761, right). Image from verified customer review.](http://images.novusbio.com/images2/VSIG1-Antibody-Western-Blot-NBP1-81074-img0005.jpg)
![Western Blot: APC Antibody [NB100-91662] - Lane1:Hela cell lysate. Lane2:HEK293T cell lysate. Lane3:Rat testis tissue lysate.](http://images.novusbio.com/images2/APC-Antibody-Western-Blot-NB100-91662-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: APC Antibody [NB100-91662] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-APC Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/APC-Antibody-Immunocytochemistry-Immunofluorescence-NB100-91662-img0006.jpg)
![Immunohistochemistry-Paraffin: APC Antibody [NB100-91662] - IHC-P analysis of a formalin fixed paraffin embedded tissue section of human colon cancer xenograft using 1: 50 dilution of APC antibody.](http://images.novusbio.com/images2/APC-Antibody-Immunohistochemistry-Paraffin-NB100-91662-img0005.jpg)
![Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-22106] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-PPAR gamma 1:800 in non-fat milk. Lane 2-7 increasing protein concentration, undifferentiated adipocytes. Lane 8-13 increasing protein concentration, differentiated adipocytes. This image was submitted via customer review.](http://images.novusbio.com/images2/PPAR-gamma-NR1C3-Antibody-Western-Blot-NBP2-22106-img0003.jpg)
![Immunohistochemistry-Paraffin: PPAR gamma Antibody [NBP2-22106] - IHC analysis of PPAR gamma in mouse liver.](http://images.novusbio.com/images2/PPAR-gamma-Antibody-Immunohistochemistry-Paraffin-NBP2-22106-img0001.jpg)
![Western Blot: PPAR gamma Antibody [NBP2-22106] - Western blot analysis of PPAR gamma in 1. human adipose and 2. human adrenal lysate.](http://images.novusbio.com/images2/PPAR-gamma-Antibody-Western-Blot-NBP2-22106-img0002.jpg)
![Western Blot: TSPAN8/TM4SF3 Antibody (1E5) [H00007103-M02] - Analysis of TSPAN8 expression in transfected 293T cell line by TSPAN8 monoclonal antibody (M02), clone 1E5.Lane 1: TSPAN8 transfected lysate (Predicted MW: 26.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/images2/TSPAN8-TM4SF3 Antibody (1E5)-Western Blot-H00007103-M02-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: TSPAN8/TM4SF3 Antibody (1E5) [H00007103-M02] - Analysis of monoclonal antibody to TSPAN8 on 293 cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/images2/TSPAN8-TM4SF3-Antibody-(1E5)-Immunocytochemistry-Immunofluorescence-H00007103-M02-img0004.jpg)
![Western Blot: TSPAN8/TM4SF3 Antibody (1E5) [H00007103-M02] - detection against Immunogen (36.67 KDa).](http://images.novusbio.com/images2/TSPAN8-TM4SF3-Antibody-(1E5)-Western-Blot-H00007103-M02-img0001.jpg)
![Western Blot: Axin-2 Antibody [NBP1-76526] - Mouse lung lysate at 1 ug/mL.](http://images.novusbio.com/images2/Axin-2-Antibody-Western-Blot-NBP1-76526-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Axin-2 Antibody [NBP1-76526] - HepG2 cells with AXIN2Antibody at 20 ug/ml.](http://images.novusbio.com/images2/Axin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76526-img0013.jpg)
![Immunohistochemistry: Axin-2 Antibody [NBP1-76526] - Mouse lung tissue antibody at 5 ug/mL.](http://images.novusbio.com/images2/Axin-2-Antibody-Immunohistochemistry-NBP1-76526-img0012.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")