A33/GPA33 Antibodies

HAF017). A specific band was detected for A33 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for A33 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human A33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3080) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for A33 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (F0107)." class="big_lightbox"> AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (F0107)." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF008). A specific band was detected for A33 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF008). A specific band was detected for A33 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cells. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human A33 Monoclonal Antibody (Catalog # MAB30804) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for A33 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

MAB1050, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> MAB1050, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

MAB30804). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox"> MAB30804). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0105B)." class="big_lightbox"> MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0105B)." alt="HT-29 human colon carcinoma cell line was stained with Rat Anti-Human A33 Monoclonal Antibody (Catalog # MAB3080, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0105B)." class="big_thumb" />

MAB1050, open histogram), followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (F0111)." class="big_lightbox"> MAB1050, open histogram), followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (F0111)." alt="HT-29 human colon carcinoma cell line was stained with Rabbit Anti-Human A33 Monoclonal Antibody (Catalog # MAB30803, filled histogram) or isotype control antibody (MAB1050, open histogram), followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (F0111)." class="big_thumb" />

HAF018). A specific band was detected for A33 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for A33 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

HAF008). A specific band was detected for A33 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF008). A specific band was detected for A33 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human A33 Monoclonal Antibody (Catalog # MAB11365) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for A33 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

F0111). View our protocol for Staining Membrane-associated Proteins. View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> F0111). View our protocol for Staining Membrane-associated Proteins. View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0102B). Staining was performed using our Staining Membrane-Associated Proteins protocol." class="big_lightbox"> MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0102B). Staining was performed using our Staining Membrane-Associated Proteins protocol." alt="HT-29 human colon carcinoma cell line was stained with Mouse Anti-Human A33 Monoclonal Antibody (Catalog # MAB30801, filled histogram) or Mouse IgG2B isotype control antibody (MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0102B). Staining was performed using our Staining Membrane-Associated Proteins protocol." class="big_thumb" />