Aromatase Antibody (H4)

Product Discontinued

Aromatase Antibody (H4) Summary

• Immunogen: Synthetic peptide corresponding to amino acids 376 - 390 of human Aromatase Swiss-Prot ID: P11511
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CYP19A1
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Western Blot 1:250
• Application Notes: Immunohistochemistry and Western Blot. In WB detects a band of approx. 55 kD in human placental extracts. P450 arom is highly expressed in placental tissue. For tissues where there may be low expression of P450 arom, the use of microsomal extracts may improve the staining for Western blots

Reactivity Notes

Marmoset monkey, Baboon, Great fruit eating bat, Collared peccary and Goat. Does not react in Giraffee. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tissue Culture Supernatant
• Preservative: 0.09% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for Aromatase Antibody (H4)

• ARO1
• ARO1EC 1.14.14.1
• Aromatase
• AROsubfamily XIX (aromatization of androgens)
• CYAR
• CYARMGC104309
• CYP19
• CYP19A1
• CYPXIX
• cytochrome P450, family 19, subfamily A, polypeptide 1
• Cytochrome P-450AROM
• EC 1.14.14.14
• Estrogen synthase
• microsomal monooxygenase

Background

Aromatase is a member of the cytochrome P450 superfamily, whose function is to produce estrogens by aromatizing androgens. The primary function of Aromatase is to convert androstenedione to estrone and testosterone to estradiol. This protein is also a key enzyme in steroidogenesis, specifically for the biosynthesis of estrogens. Because estrogens also promote certain cancers and other diseases, aromatase inhibitors are frequently used to treat such diseases. It also plays an important role in sexual differentiation, fertility, and carcinogenesis.

Aromatase is localized in the endoplasmic reticulum of the cell and its activity is regulated by tissue specific promoters that are in turn controlled by hormones, cytokines, and other factors. It can be found in many tissues reproductive tissues including gonads, brain, adipose tissue, placenta, blood vessels, skin, bone, endometrium as well as in tissue of endometriosis, uterine fibroids, breast cancer, and endometrial cancer.

Many environmental chemicals may influence aromatase activity and thereby disrupt endocrine function. The activity is increased by environmental factors such as age, obesity, insulin, gonadotropins, and alcohol but decreased by prolactin, anti-m llerian hormone, and smoking.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CYP19A1