5-Lipoxygenase [p Ser523] Antibody - Azide Free

Western Blot: 5-Lipoxygenase [p Ser523] Antibody [NB300-277] - Western blot of rat cortex lysate showing specific immunolabeling of the ~80k doublet of 5-LO phosphorylated at Ser523 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the 5-LO Ser523 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

5-Lipoxygenase [p Ser523] Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit 5-Lipoxygenase [p Ser523] Antibody - Azide Free (NB300-277) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser523 conjugated to KLH. Accession # P09917
• Modification: p Ser523
• Localization: Cytoplasmic
• Specificity: Specific for endogenous levels of the ~80 kD 5-Lipoxygenase phosphorylated at Ser523 in Western blots. Immunolabeling is completely eliminated by blot treatment with lambda phosphatase (lambda-phosphatase, 1200 units for 30 min).
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ALOX5
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for 5-Lipoxygenase [p Ser523] Antibody - Azide Free

• 5Lipoxygenase
• 5-Lipoxygenase
• 5-LO
• 5-LOX
• 5LPG
• ALOX5
• arachidonate 5-lipoxygenase
• arachidonic 5-lipoxygenase alpha-10 isoform
• arachidonic 5-lipoxygenase delta-10-13 isoform
• arachidonic 5-lipoxygenase delta-13 isoform
• arachidonic 5-lipoxygenase delta-p10 isoform
• arachidonic acid 5-lipoxygenase
• EC 1.13.11
• EC 1.13.11.34
• leukotriene A4 synthase
• LOG5
• LOX5A
• MGC163204

Background

The enzyme 5-lipoxygenase (5-LO) play a key role in regulating the production of leukotrienes (LTs) (Funk, 2001). Overproduction of LTs contribute to several diseases, most notably chronic inflammatory diseases, including asthma (Drazen et al., 1994) fibrosis (Wilborn et al., 1996) and atherosclerosis (Dwyer et al., 2004). Recent work has demonstrated that the activity of 5-LO is regulated by PKA phosphorylation of serine523 in 5-LO (Luo et al., 2004). Under normal conditions, this phosphorylation may be important in limiting inflammation. Abnormal signaling through cAMP and PKA could contribute to a variety of diseases, including those characterized by chronic inflammation. The phospho-specific antibody to ser523 on 5-LO is thus likely to provide a valuable tool for studies of the role of 5-LO regulation in diseases such as asthma, fibrosis, and atherosclerosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALOX5
• Entrez:
  • 240(Human)
  • 25290(Rat)
• Uniprot:
  • P09917(Human)