5-HT7 Antibody

Immunohistochemistry-Paraffin: 5-HT7 Antibody [NBP1-46598] - Sections of rat brain. Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 Aum.

Immunohistochemistry-Paraffin: 5-HT7 Antibody [NBP1-46598] - Sections of rat brain. Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.

• Reactivity: Hu, Mu, Rt, Gp
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

5-HT7 Antibody Summary

• Immunogen: A synthetic peptide from human 5-HT7 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat, mouse and guinea pig.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HTR7
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 1:1000
  • Western Blot 1:1000
• Application Notes: The optimal dilution should be determined by the end user. Although not tested this antibody may be useful in Immunohistochemistry-Frozen sections. Use in ICC/IF reported in scientific literature (PMID 28690143).
• Control Peptide:

  5-HT7 Protein (NBP1-46598PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from whole antisera
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Alternate Names for 5-HT7 Antibody

• 5HT7
• 5-HT7
• 5-HT-7
• 5-HT75-hydroxytryptamine receptor 7
• 5-HT-X
• 5-hydroxytryptamine (serotonin) receptor 7 (adenylate cyclase-coupled)
• HTR7
• serotonin 5-HT-7 receptor
• Serotonin receptor 7

Background

Receptors for serotonin (5-hydroxytryptamine, 5-HT) are classified into seven major classes (5-HTR1-7), based on structural, functional and pharmacological criteria (Hoyer et al, 1994). The 5-HT7 receptor (5-HT7R) is a seven-transmembrane-domain G-protein-coupled receptor that has important roles in regulating diverse biological process in the central and peripheral nervous systems (reviewed in Hedlund and Sutcliffe, 2004). Receptors for serotonin (5-hydroxytryptamine, 5-HT) are classified into seven major classes (5-HT1-7), based on structural, functional and pharmacological criteria (Hoyer et al, 1994). Sequence alignment shows a high degree of interspecies 5-HT7R homology (>90%), and a low homology with other 5-HTRs (

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 5-HT7 Antibody (NBP1-46598)(5)

Publications using NBP1-46598

• Demireva EY, Xie H, Flood ED et al. Creation of the 5-hydroxytryptamine receptor 7 knockout (5-HT7KO) Rat as a Tool for Cardiovascular Research Physiol. Genomics 2019-05-24 [PMID: 31125290] (Rat)
• Santello M, Bisco A, Nevian NE et al. The brain-penetrant 5-HT7 receptor agonist LP-211 reduces the sensory and affective components of neuropathic pain Neurobiol. Dis. 2017-07-06 [PMID: 28690143] (ICC/IF, Mouse)
• Altabella L, Sbriccoli M, Zoratto F. Differential responses to acute administration of a new 5-HT7-R agonist asa function of adolescent pre-treatment: phMRI and immuno-histochemicalstudy name: Frontiers in behavioral neuroscience et al. 2014-01-01 [PMID: 25565998] (IHC-P, Rat)
• Altabella L, Sbriccoli M, Zoratto F et al. Differential responses to acute administration of a new 5-HT7-R agonist as a function of adolescent pre-treatment: phMRI and immuno-histochemical study. Front Behav Neurosci. 2015-01-08 [PMID: 25565998] (IHC-P, Rat)
• Zhiyong Yang, Xijin Liu, Yaping Yin, Shenggang Sun, Xuejun Deng, Involvement of 5-HT7 receptors in the pathogenesis of temporal lobe epilepsy, European Journal of Pharmacology, 101016/jejphar201204011. Involvement of 5-HT7 receptors in the pathogenesis of temporal lobe epilepsy, . European Journal of Pharmacology, 10.1016/j.ejphar.2012.04.011. 2012-01-01 [PMID: 22543085] (IF/IHC, Rat)

Bioinformatics

• Gene Symbol: HTR7
• Entrez:
  • 100135549(Guinea Pig)
  • 3363(Human)
  • 15566(Mouse)
  • 65032(Rat)
• Uniprot:
  • P34969()